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Showing: 9301 - 9350 of 29620 results

  1. YSCD44A-c002

    Plasmid
    #175493
    Purpose
    Protein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Single chain Fv CD44 antibody (H90)
    Use
    Tags
    3XFLAG-EK-His8 and pelB
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175493-plasmid-map-sequence-id-353166

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  3. pKB225

    Plasmid
    #170015
    Purpose
    Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    Peptide display protein with no C-terminal peptide
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    Nov. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170015-plasmid-map-sequence-id-335287

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  5. pRSET-XS-VWF

    Plasmid
    #64847
    Purpose
    Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus
    Depositor
    Sriram Neelamegham
    Article
    Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.
    Insert
    Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
    Use
    Tags
    7X His, Cerulean, and Venus
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64847-plasmid-map-sequence-id-109340

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  7. H2AFY2

    Plasmid
    #39052
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    H2AFY2 (MACROH2A2 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pILxR5

    Plasmid
    #149497
    Purpose
    Broad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.
    Depositor
    Christopher Reisch
    Article
    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629
    Insert
    LuxR-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLuxB
    Available since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    149497-plasmid-map-sequence-id-315035

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  20. pAV106

    Plasmid
    #63196
    Purpose
    pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    63196-plasmid-map-sequence-id-104896

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  22. p6268 pDEST-59-His-Trx-HPV16E6

    Plasmid
    #24126
    Depositor
    Peter Howley
    Insert
    HPV16 E6 (E6, E6 HPV)
    Use
    Tags
    His and Trx
    Expression
    Bacterial and Mammalian
    Mutation
    none
    Promoter
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  28. NMT1

    Plasmid
    #25274
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NMT1:T108-Q496 (NMT1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. pMM627

    Plasmid
    #112532
    Purpose
    pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR
    Depositor
    Timothy Lu
    Article
    Mimee et al Science. 2018 May 25;360(6391):915-918.
    Inserts
    HrtR
    ChuA
    luxCDABE
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23107, PL(HrtO), and ProD
    Available since
    Feb. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    112532-plasmid-map-sequence-id-217573

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  34. PTPN14

    Plasmid
    #38944
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN14 (PTPN14 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pR6K-GFP-RFP

    Plasmid
    #199652
    Purpose
    R6K backbone with Golden Gate (GG) cloning sites
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199652-plasmid-map-sequence-id-412250

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  42. pBbB8k-csg-NbMS2

    Plasmid
    #171701
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171701-plasmid-map-sequence-id-336474

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  45. UHRF1

    Plasmid
    #28163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UHRF1 (UHRF1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    tandem tudor domain
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  46. MERS-coV-NSP3-c001

    Plasmid
    #173085
    Purpose
    Expression of recombinant fusion protein in E. coli
    Depositor
    Lari Lehtiö
    Article
    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012
    Insert
    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    Use
    Tags
    mCerulean
    Expression
    Bacterial
    Mutation
    D1109-D1275, codon optimized
    Promoter
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    173085-plasmid-map-sequence-id-349919

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  47. pET mRuby2 LIC cloning vector (H6-mRuby2)

    Plasmid
    #86934
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mR…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86934-plasmid-map-sequence-id-174750

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  48. pNSSCPG

    Plasmid
    #183187
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183187-plasmid-map-sequence-id-366657

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  49. pTSlb-wt

    Plasmid
    #60728
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Promoter
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60728-plasmid-map-sequence-id-99370

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Showing: 9301 - 9350 of 29620 results