32,671 results
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Plasmid#39095PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only
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pET15b-sfGO-Matryoshka-LE-LP
Plasmid#100018PurposeBacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkersDepositorInsertcpsfGFP-LSSmOrange
Tags6x HIS tagExpressionBacterialMutationLeu and Glu N-term linker and Leu and Pro C-term …PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_MBP-nLucCP-67/68
Plasmid#113922PurposePlasmid encoding the bacterial expression of the MBP fused NanoLuc circular permuted between the position 67 and 68DepositorInsertMBP-NanoLucCP-67/68
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
ExpressionBacterial and YeastPromoterpombe TEFAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHis8-p100(1-899)
Plasmid#44735DepositorAvailable SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQmod2E-GG
Plasmid#191345PurposeClostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTopaz-pBAD
Plasmid#54841PurposeLocalization: Protein Expression Vector , Excitation: 514, Emission: 527DepositorTypeEmpty backboneTags6xHis and mTopazExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSUMO His6 SUMO PYL1 (36-211)
Plasmid#177843PurposeBacterial Expression of PYL1DepositorAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
human p53-(1-393)-N268D
Plasmid#24861DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_del321-343
Plasmid#98676PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with a deletion of AA 321-343DepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST39-Ubc13-MMS2
Plasmid#184807PurposeBacterial expression of yeast UEV-E2 complexDepositorInsertUbc13, MMS2
TagsHis6ExpressionBacterialPromoterT7Available SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-4D
Plasmid#178672PurposeBacterial expression of N-terminally 6His tagged A1-LCD with four Asp residues removed (LCD: low-complexity domain, aa 186-320)DepositorAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5DL491-492
Plasmid#198290PurposeProduces PVCs retargeted with non-binding mutant of Ad5 knobDepositorInsertPVCpnf_pvc13-Ad5DL491-492
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PatoL-OG241
Plasmid#72841PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.DepositorInsertPatoL
ExpressionBacterialPromoterPatoLAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat5B-SH2
Plasmid#46516DepositorInsertStat5B (STAT5B Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 593-692PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMS30: pTarget(PmcCAST)
Plasmid#168163PurposeTarget plasmid containing PmcPSP1 and attachment site for PmcCAST.DepositorInsertsPAM for PmcCAST + PmcPSP1
tRNA-Val
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLZL2386
Plasmid#48682PurposepET29a_PPP CODA PFV Integrase with C-terminal His tag (GPG removed from pLZL2373)DepositorInsertPFV Integrase
TagsHisExpressionBacterialAvailable SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBBR6k-J23101-GFPuv
Plasmid#106390PurposepBBR6k-GFPuv with Anderson promoter J23101 expressing lacIDepositorInsertGFPuv
ExpressionBacterialPromoterLlacO-1Available SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINTF
Plasmid#41114DepositorTypeEmpty backboneExpressionBacterial, Insect, and Mamm…Available SinceMarch 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
ExpressionBacterial and YeastPromoterREV1Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCPP5951
Plasmid#128719PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopR1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B
Plasmid#157809PurposeExpression of human histone H2BDepositorInsertH2B
ExpressionBacterialPromoterT7Available SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMG81-MBP-t-M.MpeI-N374K-His
Plasmid#197985PurposeExpresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.DepositorInsertMBP-t-M.MpeI-N374K-His
TagsHis and MBPExpressionBacterialMutationM.MpeI-N374KAvailable SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-TEM1(S70A,D179G)/csTEM1
Plasmid#81163PurposepSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1DepositorInsertTEM-1 beta-lactamase
TagsCodon swapped TEM-1 from H26 to W290 and His6xExpressionBacterialMutationDeleted the sec transport tag. Plasmid encodes H2…Promoterlac promoterAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEE060
Plasmid#176817PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert611
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB232
Plasmid#170019PurposeEncodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CRAMP
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXN_Thioredoxin
Plasmid#109778PurposeProtein expression and purification of TXN_ThioredoxinDepositorInsertTXN_Thioredoxin (TXN Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
Tags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMYO127TAG-SepT
Plasmid#34625DepositorInsertMyoglobin-127TAG
Tags6xHISExpressionBacterialMutationcodon-optimized + an amber stop codon was introdu…PromoterIppAvailable SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1 U1-C-FL
Plasmid#129077Purposegeneration of GST-tagged U1-C protein using bacteriaDepositorInsertU1-C (SNRPC Human)
ExpressionBacterialAvailable SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1 Sesn2
Plasmid#61873Purposebacterial expression of a GST-Sesn2 (mouse)DepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Clover-pBAD
Plasmid#54575PurposeLocalization: Protein Expression Vector , Excitation: 505, Emission: 515. This plasmid encodes Clover H231L based on reference from Bajar et. al. Sci Rep. 2016.DepositorTypeEmpty backboneTags6xHis and CloverExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)
Plasmid#159687PurposeE. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCOM4
Plasmid#49798PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneTagsS-tagExpressionBacterialPromoterT7Available SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
SNX29-PX (269-391)
Plasmid#119108PurposeBacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)DepositorInsertSNX29-PX (269-391)
TagsGSTExpressionBacterialPromotertacAvailable SinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-BmNG
Plasmid#232207PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-BSD-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-CT-PmNG
Plasmid#232201PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mNeongreen-3xTy-T2A-PAC-3xTyDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM8315
Plasmid#213988PurposePrpsM driven expression of mCherry2-LDepositorInsertmCherry2-L
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1_CylLS_CylM
Plasmid#208760PurposeExpresses modified cytolysin S (mCylLS) in E. coliDepositorInsertsCylLS
CylLM
TagsHisx6 TagExpressionBacterialPromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRa41_491
Plasmid#49702DepositorInsertECF41_491
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltalinker
Plasmid#157870Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the linkerDepositorAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-ATRX ADD
Plasmid#59698PurposeContains histone modification interacting domain (ATRX ADD)DepositorAvailable SinceOct. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
XOR gate-V2.0
Plasmid#44453DepositorInsertXOR-gate
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Az
Plasmid#197101PurposeTo express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanineDepositorInsertTyrRS variant, amber suppressor tRNA, kanamycin resistance gene
ExpressionBacterialMutationThr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…Available SinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp14
Plasmid#154956PurposeExpresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMal-Halo
Plasmid#209349PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagIIDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express
Plasmid#103995PurposePlasmid encoding DsRed-ExpressDepositorInsertDsRed-Express
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2A, K5E, N6D, T21S,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -