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31,059 results

Showing: 9351 - 9400 of 31059 results

  3. pTU2-A-RFP (colE1 origin)

    Plasmid
    #74093
    Purpose
    pTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    R0010 Const. Promoter controlling RFP
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74093-plasmid-map-sequence-id-138340

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  4. pGBK CUP1 mGrape3

    Plasmid
    #170265
    Purpose
    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3
    Depositor
    Bruce Hamilton
    Article
    Hernandez et al bioRxiv
    Insert
    momeric Grape 3
    Tags
    GAL4 DNA Binding Domain and Myc
    Expression
    Bacterial and Yeast
    Promoter
    CUP1
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    170265-plasmid-map-sequence-id-335101

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  8. pEGFP C3-YAP-deltaC

    Plasmid
    #21126
    Depositor
    Marius Sudol
    Article
    Tsutomu Oka & Marius et al
    Insert
    Yes-kinase associated protein (YAP1 Human)
    Tags
    EGFP-C3
    Expression
    Bacterial and Mammalian
    Mutation
    5 Carboxy-terminal amino acids -FLTWL are deleted
    Available Since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only

  12. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  17. pTSSX-STC

    Plasmid
    #115668
    Purpose
    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag
    Depositor
    David Kramer
    Article
    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459
    Insert
    small tetraheme cytochrome c
    Tags
    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    115668-plasmid-map-sequence-id-228494

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  18. pBBA57-TEV-His12

    Plasmid
    #137033
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length BBA57, contains signal pep…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137033-plasmid-map-sequence-id-275688

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  25. HDAC6 (3GV4)

    Plasmid
    #25297
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDAC6_42 (HDAC6 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acid residues 1109-1215
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pGEX-MK2

    Plasmid
    #30187
    Depositor
    Bruce Tempel
    Insert
    MK2 (Kcna2 Mouse)
    Expression
    Bacterial
    Available Since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  30. Q-ACACAA-10

    Plasmid
    #166546
    Purpose
    scFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166546-plasmid-map-sequence-id-329238

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  31. TAF1

    Plasmid
    #39118
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pOGG011

    Plasmid
    #113985
    Purpose
    Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    pBBR1
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113985-plasmid-map-sequence-id-223389

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  39. pET19-MTdTc302

    Plasmid
    #207458
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    MTdTc302 (Dntt Mouse)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser
    Available Since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207458-plasmid-map-sequence-id-407772

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  49. pET15b/GgVcl 884-1066-ECFP

    Plasmid
    #46182
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Tags
    ECFP and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (tail domain)
    Promoter
    T7
    Available Since
    July 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid
    #73223
    Purpose
    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PL22
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73223-plasmid-map-sequence-id-135055

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Showing: 9351 - 9400 of 31059 results