31,059 results
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Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
ExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available SinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-HA-BioID2-SAPAP3
Plasmid#176857PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in neuronsDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialPromoterhSyn1Available SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB092a
Plasmid#79217PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1+2); Ppro1Available SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNP561
Plasmid#222723PurposeTarget and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 RecombinaseDepositorTypeEmpty backboneTags6xHisExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIKpBAD
Plasmid#129273PurposeBroad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoterDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET32-sLatherin
Plasmid#61685Purposebacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusionDepositorInsertLatherin (LATH horse)
TagsHIS and Trx-Tag thioredoxin proteinExpressionBacterialMutationCodon optimized for E.coliPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFG036
Plasmid#137996PurposecI repressor expression plasmidDepositorInsertcI
UseSynthetic BiologyExpressionBacterialMutationThermosensitiveAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI-J23106-geneIII
Plasmid#80843PurposePhagemid (encodes M13 gene III and lambda cIopt).DepositorInsertsM13 gene III
Lambda cI
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFW5000
Plasmid#224214PurposeE. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-Ferritin-Dronpa
Plasmid#101194PurposeExpresses a Ferritin-Dronpa fusion construct in E. coliDepositorInsertFerritin-Dronpa
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialPromoterT7Available SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-CPDSacI
Plasmid#38255DepositorTypeEmpty backboneTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialPromoterT7Available SinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJJS011
Plasmid#188334PurposePCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mTagBFP2::Linker
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC011 LwCas13a locus with targeting spacer
Plasmid#91903PurposeLwCas13a locus into pACYC184 with targeting spacerDepositorInsertLwCas13a with targeting spacer
UseCRISPRExpressionBacterialAvailable SinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
Tagsstrep tagExpressionBacterialAvailable SinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 ERbeta
Plasmid#35563DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-37
Plasmid#214346PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A.2-c002
Plasmid#173089PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.2-c002
Plasmid#173088PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
TagsmCeruleanExpressionBacterialMutationQ162-N372, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
His10-CD3z-SNAPf-ybbR (XSB507)
Plasmid#78508PurposeConstruct for expressing CD3z-SNAPf-ybbR in E. ColiDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU398
Plasmid#178980Purpose13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)DepositorInsertgRAMP
TagsStrep-Tag II SUMOExpressionBacterialPromoterT7Available SinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBEC8
Plasmid#187599PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)
Plasmid#169077PurposeInsect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepIIDepositorInsertsTagsGFP tag, GGlinker, TEV cleavage site. StrepII and…ExpressionBacterial and InsectPromoterPolyhedrin promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p-1-CYB5R3 deltaN26
Plasmid#204557PurposeExpresses GST-tagged CYB5R3 deltaN26 in E.coliDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LACTB2
Plasmid#39115PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAL-EFSep
Plasmid#34627DepositorInsertEF-Sep (tufB E. coli)
Tags6xHIS and MBPExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…Available SinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJOE7771.1
Plasmid#135077PurposeMannitol, arabitol, and glucitol-inducible mtlR/PmtlE expression vectorDepositorInsertmtlR
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdTomato
Plasmid#163443PurposeBacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backboneDepositorInserttdTomato
Tags6xHisExpressionBacterialPromoterT7Available SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Aurein1.2_(+36GFP)-Cre
Plasmid#71748PurposeAurein1.2+36GFP-Cre fusion expression in E. ColiDepositorInsert(GGS)9_Aurein1.2+36GFP-Cre
Tags6XHisExpressionBacterialAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD33.1-DpnII
Plasmid#127413PurposeExpresses DpnII restriction endonuclease in bacteriaDepositorInsertDpnII
ExpressionBacterialPromoterpBADAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
Plasmid#85111Purposeexpression of recombinant human mature (delta19) ETFalpha wt without tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMVV207
Plasmid#215343PurposegRNA expression for S. stutzeri nifL deletion on an RSF1010 backboneDepositorInsertS. stutzeri nifL gRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionBacterialPromoterJ23119Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV205
Plasmid#215342PurposeRecombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backboneDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23105Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ALC1-c005
Plasmid#173092PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVRa41_491
Plasmid#49702DepositorInsertECF41_491
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProB.gfp
Plasmid#112516PurposeProB driven gfpmut3 expression with synTTDepositorInsertProB gfpmut3 TT
ExpressionBacterialPromoterProBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
ExpressionBacterialMutationN/APromoterCAT promoterAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAP003l2
Plasmid#209748PurposeRepA(N99D) SC101 T0-BS7 araC pBAD SD8 NpuC-C[AGGA]LFVY-NpuN-SsrA ProK 5 tRNA operon tetA-orfL blaDepositorInsertNpuC-C[AGGA]LFVY-NpuN-SsrA(rc)
ExpressionBacterialAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only