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31,059 results

Showing: 9901 - 9950 of 31059 results

  4. pRCPam

    Plasmid
    #195861
    Purpose
    Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.
    Depositor
    Gregory Phillips
    Article
    DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)
    Insert
    Amber Chromogenic Protein on pRCPam plasmid
    Expression
    Bacterial
    Mutation
    Gln62X
    Available Since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195861-plasmid-map-sequence-id-384172

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  8. RHOB

    Plasmid
    #38801
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOB (Rhob Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pdUA-lacIN

    Plasmid
    #141079
    Purpose
    Plasmid vector for testing DNA binding proteins by the I-Block assay.
    Depositor
    Antal Kiss
    Article
    Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28
    Type
    Empty backbone
    Use
    Vector for the i-block assay designed to study dn…
    Expression
    Bacterial
    Promoter
    Escherichia coli lacIN promoter
    Available Since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    141079-plasmid-map-sequence-id-310860

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  14. PTPRE

    Plasmid
    #38950
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRE (PTPRE Human)
    Tags
    His6
    Expression
    Bacterial
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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  16. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid
    #160881
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Expression
    Bacterial
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160881-plasmid-map-sequence-id-318841

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  22. pET mRuby2 LIC cloning vector (H6-mRuby2)

    Plasmid
    #86934
    Purpose
    pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    6xHis, AviTag (biotin), TEV cleavage site, and mR…
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86934-plasmid-map-sequence-id-174750

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  25. pJES103

    Plasmid
    #135410
    Purpose
    E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter
    Depositor
    Miriam Braunstein
    Article
    McCann et al Microbiology. 2007 Oct;153(Pt 10):3350-9
    Insert
    blaTEM-1
    Expression
    Bacterial
    Mutation
    E. coli 'blaTEM-1 with M. tuberculosis mpt63…
    Promoter
    hsp60
    Available Since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135410-plasmid-map-sequence-id-270631

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  33. USP21 (3MTN)

    Plasmid
    #28112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP21 (USP21 Human)
    Tags
    His tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    catalytic domain only (amino acid residues 209-56…
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107582
    Purpose
    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fba promoter Bacillus megaterium DSM319
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107582-plasmid-map-sequence-id-209150

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  37. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available Since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  39. p6268 pDEST-59-His-Trx-HPV16E6

    Plasmid
    #24126
    Depositor
    Peter Howley
    Insert
    HPV16 E6 (E6, E6 HPV)
    Tags
    His and Trx
    Expression
    Bacterial and Mammalian
    Mutation
    none
    Available Since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  41. pETM-11-HsPABPC1_J

    Plasmid
    #146642
    Purpose
    Bacterial Expression of HsPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    HsPABPC1 (PABPC1 Human)
    Expression
    Bacterial
    Mutation
    two silent mutations compared to the sequence giv…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146642-plasmid-map-sequence-id-396951

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  44. L57V

    Plasmid
    #34556
    Depositor
    Kevin Truong
    Insert
    L57 (CASP7 Human, A.Sativa (LOV2))
    Tags
    YFP
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic domain of caspase-7 was truncated at am…
    Promoter
    CMV, T7
    Available Since
    April 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  46. pExp-PpiB

    Plasmid
    #112572
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-PpiB and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112572-plasmid-map-sequence-id-217222

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  47. pMTB2-Avi-Cerulean-RanGAP

    Plasmid
    #79878
    Purpose
    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    Beta-actin2
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79878-plasmid-map-sequence-id-149739

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  50. pNSSCP

    Plasmid
    #183188
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Expression
    Bacterial
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183188-plasmid-map-sequence-id-366663

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Showing: 9901 - 9950 of 31059 results