Skip to main content
Addgene
Showing: 9901 - 9950 of 29646 results

  1. mOrange in pBSDONR P4r-P2

    Plasmid
    #121529
    Purpose
    For making C-terminal mOrange fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    mOrange
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    121529-plasmid-map-sequence-id-234042

    Enlarge

  6. pETM33_Nsp7

    Plasmid
    #156476
    Purpose
    Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156476-plasmid-map-sequence-id-307742

    Enlarge

  12. pE1c

    Plasmid
    #17461
    Purpose
    modified pENTR vector for C-terminal STREP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    STREP
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17461-plasmid-map-sequence-id-8629

    Enlarge

  15. pAV124

    Plasmid
    #63190
    Purpose
    pRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63190-plasmid-map-sequence-id-104887

    Enlarge

  18. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Promoter
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  24. pGBW-m4046758

    Plasmid
    #145670
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145670-plasmid-map-sequence-id-297837

    Enlarge

  26. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

    Enlarge

  27. pXY1

    Plasmid
    #90487
    Purpose
    Plasmid with thl(CA_C2873) promoter for gene expression in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052
    Depositor
    Sheng Yang
    Article
    Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    90487-plasmid-map-sequence-id-178575

    Enlarge

  28. pAAH5

    Plasmid
    #41829
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    T7,TEF
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41829-plasmid-map-sequence-id-69202

    Enlarge

  33. pUC19-link-PAmCherry-FLAG:cat

    Plasmid
    #211832
    Purpose
    Plasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.
    Depositor
    Peng Chen
    Insert
    PAmCherry-FLAG-cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    NA
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211832-plasmid-map-sequence-id-426747

    Enlarge

  36. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid
    #49520
    Purpose
    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally
    Depositor
    Dirk Schnappinger
    Article
    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.
    Insert
    T38M-P750-luxAB-DAS+4
    Use
    Tags
    DAS+4 (AANDENYSENYADAS)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49520-plasmid-map-sequence-id-73899

    Enlarge

  42. GAMT

    Plasmid
    #25494
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GAMT (GAMT Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. pTNS2

    Plasmid
    #87802
    Purpose
    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Use
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87802-plasmid-map-sequence-id-177263

    Enlarge

  48. pOP3SU

    Plasmid
    #112599
    Purpose
    Used for expression of protein in E. coli as N-terminal Sumo tag fusions
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    Sumo
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112599-plasmid-map-sequence-id-220209

    Enlarge

  49. pET21a - APX

    Plasmid
    #89618
    Purpose
    Expresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG induction
    Depositor
    Judy Lieberman
    Article
    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1
    Insert
    Ascorbate dependent Tryparedoxin peroxidase
    Use
    Tags
    6HIS and T7 tag (gene 10 leader)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    89618-plasmid-map-sequence-id-176685

    Enlarge

Showing: 9901 - 9950 of 29646 results