32,672 results
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Plasmid#169040PurposemCherry preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag in pET28a.DepositorInsertH6-spTorA-mCherry
Tags6xHis and spTorA (signal peptide of E. coli TorA)ExpressionBacterialPromoterT7Available SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialPromoterPrhaAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1-1180
Plasmid#13265DepositorAvailable SinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET29b-Ava3858-3855
Plasmid#45746DepositorInsertAva3858-3855
ExpressionBacterialPromoterT7Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF3∆165N (WT)
Plasmid#175418PurposeEncodes His-MBP-TEV-WT eRF3∆165NDepositorAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PTPN5
Plasmid#39169PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PLtetO-1-UTR1-TetR-deGFP-T500
Plasmid#45774DepositorInserttetR-deGFP
UseSynthetic BiologyTagsFusion protein of TetR and deGFP, with glycine-se…ExpressionBacterialPromoterPLtetO-1Available SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
SOCS4B-c002
Plasmid#53669Purposebacterial expression plasmidDepositorInsertSOCS4B
TagsHis6-TEVExpressionBacterialMutationBacterial expression for structure determination;…Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ1
Plasmid#59739Purposeexpresses PDZ1 domain of human ZO-1 in bacteriaDepositorAvailable SinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
NEDD4L
Plasmid#25169PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 9, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-His-hPKM2
Plasmid#42515DepositorAvailable SinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28Teb1
Plasmid#28173DepositorInsertTeb1
Tags6xhistidineExpressionBacterialAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
CBX1
Plasmid#25245PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
LHP2691 - pET30-Ub-K63A
Plasmid#32179DepositorTagsHISExpressionBacterialMutationK63APromoterT7Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LHP2690 - pET30-Ub-K48A
Plasmid#32178DepositorTagsHISExpressionBacterialMutationK48APromoterT7Available SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
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GARS_pNIC-Bio3
Plasmid#153049PurposeExpression construct for producing Avi tagged Aspartyl-tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
RAB26
Plasmid#25528PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b(+)_SNAP-p24
Plasmid#130718PurposePlasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid proteinDepositorInsertSNAP-Tag-p24
TagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HCLS1-1/1
Plasmid#91437PurposeProtein expression and purification of human SH3 domain construct HCLS1-1/1DepositorInsertHCLS1-1/1 (HCLS1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-TAZ
Plasmid#19024DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-β′-COPI-WD40
Plasmid#180753PurposeBacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).DepositorInsertβ′-COPI-WD40 (SEC27 Budding Yeast)
TagsN-terminal strep tag, His-tag, and SUMO tagExpressionBacterialMutationEncodes residues 1-301; deleted 302-889; mutated …PromoterT7Available SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHL 246
Plasmid#53014PurposeAmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1DepositorInsertGFP
UseSynthetic BiologyTagsfimS (off orientation)ExpressionBacterialAvailable SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBXC3GH
Plasmid#47070PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 WT
Plasmid#201797PurposeExpresses wild-type Tensin-2 protein in MAC-Tag-C backboneDepositorAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5400_pMBP_CDS dropout vector
Plasmid#221189PurposeExpression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interestDepositorTypeEmpty backboneTags10x His-tag + E. coli maltose binding protein (MB…ExpressionBacterialPromoterT7 promoterAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB2
Plasmid#162690PurposeExpresses DabAB2 inorganic carbon transport complexDepositorInsertDabAB2 inorganic carbon transport complex
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pep…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNRB150
Plasmid#36451DepositorAvailable SinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
4LG1
Plasmid#60092PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21D:JMC072-C04:C227980 (VCPKMT Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCD227
Plasmid#113318PurposeAraC-pBAD-dCas9, MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRExpressionBacterialMutationR93AAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR-SF61
Plasmid#163916PurposetRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codonDepositorInsertSF5Phe tRNA synthetase
ExpressionBacterialPromoteraraBADAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #3
Plasmid#104210PurposeBacterial expression of WW domain #3 from NEDD4DepositorInsertNEDD4 WW domain #3 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mEYFP
Plasmid#103985PurposePlasmid encoding mEYFPDepositorInsertmEYFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S65G, V68L, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4in
Plasmid#176232PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmA1O4out
Plasmid#176233PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30del
Plasmid#106902PurposepQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_target_WT
Plasmid#139465PurposeT7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensorDepositorInsertTarget_WT
ExpressionBacterialAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Reb_207 aka PAS630
Plasmid#71235PurposeReb_206 with RebA(S88P)DepositorInsertReb locus
TagsmNeonGreen to second copy RebB, soluble mCherry a…ExpressionBacterialMutationRebA(S88P)Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMD18-T-pyrF
Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…ExpressionBacterialAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-PTB
Plasmid#21929DepositorAvailable SinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET30b jGCaMP8f F366H
Plasmid#204139PurposeProduction of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coliDepositorInsertjGCaMP8f F366H
TagsN-terminal His tagExpressionBacterialMutationPhenylalanine 366 changed to histidinePromoterT7Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-141
Plasmid#118525PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only