32,127 results
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Plasmid#20731DepositorInsertCol1a-3'probe (Col1a1 Mouse)
ExpressionBacterialAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDSG594
Plasmid#214221Purposecompetition strain 10 integrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)
Plasmid#182395PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusionsDepositorInsertsVNp-LZ-VenusN154
VNp-LZ-VenusC155
TagsVesicle Nucleating peptide (VNp) & Leucine Zi…ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX-His(1-868, P801G)
Plasmid#186808PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).DepositorInsertALIX-His (1-868, P801G) (PDCD6IP Human)
TagsHis6ExpressionBacterialMutationProline 801 to GlycineAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD∆cat
Plasmid#81054Purposebacterial expression of catalytic dead catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
Tags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057 with histid…PromoterT7Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_minus_cer-region
Plasmid#175297PurposeProduction of HaloTag fusion proteinDepositorTypeEmpty backboneUseCell free expressionTagsHaloTag-6xHisExpressionBacterialAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Y203)
Plasmid#63559PurposeExpression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Y203)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged threonine at position 203 with respect to…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNDGG050
Plasmid#231319PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-4
Plasmid#228986PurposeFor bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-4
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaRIgG-2
Plasmid#228996PurposeFor bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaRIgG-2
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaGIgG-12
Plasmid#228999PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaGIgG-12
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-3
Plasmid#228985PurposeFor bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-3
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB134
Plasmid#228485Purpose"Empty" N-terminal 6xHis-TEV-Halotag-HRV vector for easy DNA Assembly for pulldowns (Bacterial Expression)DepositorTypeEmpty backboneTags6xHis-TEV-Halo-HRVExpressionBacterialPromoterT7 PromoterAvailable SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTBY1-T4uvsY
Plasmid#228214PurposeExpression in BL21(DE3) of T4 UvsY-like recombination mediatorDepositorInsertuvsY (uvsY Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-AffiHER2-SpyTag
Plasmid#216280PurposeExpresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expressionDepositorInsertSnoopTag-AffiHER2-SpyTag
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, SpyTag, and Thrombin cleavage sit…ExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDB.His.MBP.3C CARD8 CARD WT
Plasmid#164022PurposeBacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARDDepositorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2E
Plasmid#75291PurposeExpresses proteins in the cytoplasm as fusions to maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable SinceJuly 20, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTn7xTS-dTomato
Plasmid#117391PurposeTn7 tagging vector pTn7xTS with dTomato expression scaffoldDepositorInsertdTomato
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-4
Plasmid#13001DepositorInsertTSP-4 (THBS4 Human)
ExpressionBacterialAvailable SinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2d
Plasmid#165103Purposebacterial expression of a GST fusion of Human Nedd4.2dDepositorInsertNedd4.2d (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCsy4
Plasmid#44252DepositorInsertcsy4 gene
UseSynthetic BiologyExpressionBacterialPromoterpLtetO-1Available SinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Himar-131
Plasmid#185923PurposeHimar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein geneDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EC10161
Plasmid#154060PurposeLevel 0.5 Golden Gate backbone, containing loxP sites.DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)
Plasmid#169077PurposeInsect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepIIDepositorInsertsTagsGFP tag, GGlinker, TEV cleavage site. StrepII and…ExpressionBacterial and InsectPromoterPolyhedrin promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJS008
Plasmid#188331PurposePCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::wrmScarlet::mIAA7
ExpressionBacterialAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
TagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG169
Plasmid#124367PurposeXylose inducible system with the CRE site for expression in B. subtilisDepositorAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRExpressionBacterialAvailable SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPath-BGC08-SGE
Plasmid#200006PurposeBGC08 pathway. SGEDepositorInsertPathways
ExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFPV25
Plasmid#20667DepositorTypeEmpty backboneTagsGFP mut3ExpressionBacterialAvailable SinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT1002
Plasmid#8621DepositorInsertbarnase, barstar
Tagsas pMT416ExpressionBacterialMutationCI157 lambda repressor gene has two HindIII sites…Available SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S
Plasmid#238003PurposeExpression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cellsDepositorTagsS and T7ExpressionBacterialAvailable SinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO1125
Plasmid#235742PurposeProtein expression of GST-tagged PX domain (Cys at 2) in bacteriaDepositorAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGal-Trans-Myc-eGFP-KDEL
Plasmid#177729PurposeER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc taggedDepositorInsertcollagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
TagsKDEL, Myc, and eGFPExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
R701-X72-527: His6-tev-Hs.CALM1(76-148)
Plasmid#159695PurposeE. coli protein expression of His6-tev-Hs.CALM1(76-148)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513-iNicSnFR3b-(V7)
Plasmid#124881PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3b-(V7)
TagsHA Tag, His Tag, and NAExpressionBacterialMutationNAPromoterT7Available SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD33.1-DpnII
Plasmid#127413PurposeExpresses DpnII restriction endonuclease in bacteriaDepositorInsertDpnII
ExpressionBacterialPromoterpBADAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 8, MG-26-21)
Plasmid#110745PurposeBacterial expression of human USP30 (construct 8)DepositorInsertUSP30 (USP30 Human)
TagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350D, I353E + insertion deletion: 64-357;…PromoterT7Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRU1099
Plasmid#14464DepositorInsertunstable gfp-mut3.1 AAV
ExpressionBacterialAvailable SinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only