32,684 results
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Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
A0A075HNX4
Plasmid#177695Purposeexpresses a fatty alcohol oxidase in E. coliDepositorInsertLong chain alcohol oxidase
Tags10xHisExpressionBacterialPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Sox2
Plasmid#136612PurposeDox-inducible lentiviral vector expressing mouse Sox2DepositorAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046871
Plasmid#145724PurposeBacterial Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
csr-1_son-1_mTagBFP2
Plasmid#191746PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
TagsNCU04502 promoter and mTagBFP2ExpressionBacterialAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-His-APEX2
Plasmid#201471PurposeRecombinant soluble APEX2 for SLAPSHOT labelingDepositorInsertAPEX2
Tags6xHisExpressionBacterialAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSPB845
Plasmid#128296Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPLlacO1 - lp-cfp-parB, BBa_J23110 - lacI
ExpressionBacterialAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_C5
Plasmid#171925PurposeBacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_C5
TagsHis6ExpressionBacterialAvailable SinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
NpT7-5-Erk2
Plasmid#39230DepositorAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A321G
Plasmid#98670PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A321GDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET23a-HsUBB
Plasmid#69562PurposeBacterial expression; in vitro transcription/translation (T7)DepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHlyIIR-H1
Plasmid#74681PurposeHlyIIR-H1 gateDepositorInsertPTac-RBSH1-HlyIIR-PHlyIIR-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1gamma
Plasmid#51770PurposeExpresses PP1 gamma residues 7-323DepositorInsertpp1 gamma
TagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 gamma residues 7-323PromoterT7-lacAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS chick islet1 [TJ#44]
Plasmid#16273DepositorInsertislet1 (ISL1 Chicken)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-TEV-PWWP_DNMT3A
Plasmid#186970PurposeBacterial expression of human DNMT3A PWWP domain (residues 278–427) with His-MBP tag and TEV protease cleavage site.DepositorInsertDNMT3A PWWP domain (DNMT3A Human, Synthetic)
TagsHis-MBPExpressionBacterialMutationPWWP domain (residues 278–427)PromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
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DNMT3b KO Neo
Plasmid#16651DepositorInsertDNMT3b knock-out construct (DNMT3B Human)
ExpressionBacterialAvailable SinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_09
Plasmid#194670PurposeProtein production of the split HaloTag based calcium recorder Caprola_09 in bacteriaDepositorInsertCaprola_09
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Z-VCP-10
Plasmid#166559PurposescFv of a human scaffold targeting Transitional endoplasmic reticulum ATPase. Antigen coverage aa 1-806 of 806, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SS13 lac-inducible GFP reporter circuit
Plasmid#30336DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET a MatryoshCaMP6s
Plasmid#100019PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNDGG004
Plasmid#231317PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Tc-par-ELT4; GG assembly from pOGG011, pOGG012, pOGG014, pOGG042, and pOGG004, TcRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG051
Plasmid#231320PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG053
Plasmid#231322PurposeBEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG011, pOGG012, and pOGG014, NmR/KmRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMBA328
Plasmid#214748PurposeMedium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
ExpressionBacterialAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-ISG15 C78SN13Y
Plasmid#165106Purposebacterial expressio of a GST fusion of Human ISG15 C78SN13YDepositorInsertISG15 (ISG15 Human)
TagsGST from plasmid.ExpressionBacterialMutationC78SN13Y; The sequence of ISG15-C78S/N13Y contai…Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-1066 (full length)
Plasmid#46171DepositorAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJMP1223
Plasmid#119261Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1221
Plasmid#119260Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
TagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPA3
Plasmid#24777DepositorTypeEmpty backboneUseIntegrating vectorExpressionBacterialAvailable SinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCON1-ProD.mCherry
Plasmid#112522PurposeProD driven mCherry expression with synTTDepositorInsertProD mCherry TT
ExpressionBacterialPromoterProDAvailable SinceJuly 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-Pdp1
Plasmid#198320PurposeFor affinity purification of Pdp1 payloadDepositorInsertTwinStrep-SUMO-Pdp1
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBH546_Tet-Cas14b1Locus
Plasmid#112503PurposeExpresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoterDepositorInsertCas14b1
UseCRISPRExpressionBacterialPromoterTetAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
IBMc090
Plasmid#161937PurposeCarries engineered transposon for IBMDepositorInsertpTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
ExpressionBacterialAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
JpExpress404 PTEN
Plasmid#49420PurposeBacterial Expression VectorDepositorAvailable SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-6xHis-BglII
Plasmid#196654PurposeDerived from pGLO (BioRad) by the insertion of a 6xHis Tag and a BglII siteDepositorTypeEmpty backboneTags6xHis tagExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #2
Plasmid#104205PurposeBacterial expression of WW domain #2 from NEDD4LDepositorInsertNEDD4L WW domain #2 (NEDD4L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE30-rSyntenin1
Plasmid#89428Purposebacterial expression of rat Syntenin1DepositorInsertSDCBP
TagsHisExpressionBacterialPromoterT5Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpal2At
Plasmid#78286PurposeExpresses A. thaliana PAL2 in E. coliDepositorAvailable SinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ4M_hnRNPA2_FL_D290V
Plasmid#139110PurposeExpress FL hnRNPA2 D290V with a C-terminal MBP tagDepositorAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK131
Plasmid#71548PurposeProduces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)DepositorInsertAlanine racemase
ExpressionBacterialPromoterT7Available SinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEND-193_Cacnes_HS
Plasmid#225622PurposeC. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)DepositorInsertHspR
UseSynthetic BiologyTagspFAST-6XHisExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-229_Cacnes_EforCP
Plasmid#225615PurposeReplicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCPDepositorInsertEforCP
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
BPTI-[5,55]Gly14Ile38
Plasmid#195059PurposeThermal stability of BPTIDepositorInsertbovine pancreatic trypsin inhibitor
TagsHis tag and trpΔLE leader sequencesExpressionBacterialMutationChanged Alanine residues at position of 14 and 38…PromoterT7 promotorAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSR:MSMEG_0835:Dendra
Plasmid#174923PurposeC-term fluorescent fusionDepositorInsertMSMEG_0835
UseIntegrating mycobacterial expression vector attl5…TagsGly-Ala Linker/Dendra2/1x Flag TagExpressionBacterialPromoterpmycTetOAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Human, Synthetic)
TagsHis-GSTExpressionBacterialPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
mNG-sfTq2
Plasmid#124218PurposeCytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2DepositorInsertmNG-sfTq2
ExpressionBacterialAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only