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Plasmid#52171PurposeEncodes module 8 with amino acids 12C-16Q for recognition of nt 1ADepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in module 8PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
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PET-SpCas9-HF1-plus-NLS-6xHis
Plasmid#207381PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1-plus
UseCRISPRTags6xHisExpressionBacterialMutationN497A, Q695A, Q926A, amino acids 1005-1013 replac…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-SpCas9-HF1-NLS-6xHis
Plasmid#207385PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-SpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926A, amino acids 1005-1013…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1-Venus SX
Plasmid#103990PurposePlasmid encoding Venus SXDepositorInsertVenus SX
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal Kat9-5
Plasmid#104031PurposePlasmid encoding 2ndVal Katushka9-5DepositorInsert2ndVal Katushka9-5
UseLow copyTagsExpressionBacterialMutationKatushka9-5 with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB2-2ndVal mCherry-L
Plasmid#104026PurposePlasmid encoding 2ndVal mCherry-LDepositorInsert2ndVal mCherry-L
UseLow copyTagsExpressionBacterialMutationmCherry-L with valine inserted in 2nd positionPromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-coSCFP1
Plasmid#104014PurposePlasmid encoding coSCFP1DepositorInsertcoSCFP1
UseLow copyTagsExpressionBacterialMutationSame protein sequence as SCFP1 (codon optimized n…PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-comVenJBC
Plasmid#104020PurposePlasmid encoding comVenJBCDepositorInsertcomVenJBC
UseLow copyTagsExpressionBacterialMutationSame protein sequence as mVenus JBC (codon optimi…PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-comVenME
Plasmid#104021PurposePlasmid encoding comVenMEDepositorInsertcomVenME
UseLow copyTagsExpressionBacterialMutationSame protein sequence as mVenus ME (codon optimiz…PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-coClover
Plasmid#104022PurposePlasmid encoding coCloverDepositorInsertcoClover
UseLow copyTagsExpressionBacterialMutationSame protein sequence as Clover (codon optimized …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-comClover3
Plasmid#104023PurposePlasmid encoding comClover3DepositorInsertcomClover3
UseLow copyTagsExpressionBacterialMutationSame protein sequence as mClover3 (codon optimize…PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus JBC
Plasmid#103987PurposePlasmid encoding mVenus JBCDepositorInsertmVenus JBC
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-SCFP1
Plasmid#103969PurposePlasmid encoding SCFP1DepositorInsertSCFP1
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJUMP21-1A(sfGFP)
Plasmid#126968PurposeLevel 1 vector. sfGFP reporter. Origin R6K (pir conditional).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSM17
Plasmid#101641PurposeEncodes the fluorescent protein eqFP486 for constitutive expression in E. coli.DepositorInserteqFP486
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-BAT-RED
Plasmid#20674DepositorInsertWnt reporter dsRED
UseTagsNLSExpressionBacterialMutationPromoterAvailable sinceMay 26, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS101
Plasmid#118280PurposeExpresses GFPmut2 with low cell-to-cell variation on a low-copy plasmid and tetracycline inductionDepositorInsertGFPmut2
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDUET-aaCPRct-A13AMO
Plasmid#20116DepositorInsertp450 reductase and Amorphadiene oxidase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
PGEX 4T-1-PPARgamma2
Plasmid#78773PurposeTo purify GST-tagged recombinant proteinDepositorInsertPPAR_2
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PPP1R13L-1/1
Plasmid#91509PurposeProtein expression and purification of human SH3 domain construct PPP1R13L-1/1DepositorInsertPPP1R13L-1/1 (PPP1R13L Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPotef-LN22*-3xGNLS-cbx
Plasmid#86780PurposeExpresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.DepositorInsertLN*
UseUstilago maydis expressionTagsGfpExpressionBacterialMutationPromoterPotefAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PLAT_Trypsin
Plasmid#110039PurposeProtein expression and purification of PLAT_TrypsinDepositorInsertPLAT_Trypsin (PLAT Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-TRC8 513-664
Plasmid#185352PurposeRING domain-containing GST fusion protein for ubiquitination assays.DepositorInsertTRC8 (RNF139 Human)
UseTagsGSTExpressionBacterialMutationaa 513-664 onlyPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-HRD1 aa 236-444
Plasmid#185349PurposeRING domain-containing GST fusion protein for ubiquitination assays.DepositorInsertHRD1/SYVN (SYVN1 Human)
UseTagsGSTExpressionBacterialMutationaa 236-444 onlyPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(800-868)-strep
Plasmid#180023PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.DepositorInsertGB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2J1 1-283
Plasmid#185348PurposeIn vitro transcription and translation in bacterial systemDepositorInsertUBE2J1 (UBE2J1 Human)
UseTagsExpressionBacterialMutationCytoplasmic domain only, aa 1-283PromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 AUP1 292-410
Plasmid#185331PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 292-410 onlyPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)-Strep
Plasmid#164444PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to GlycinePromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationD27NPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_WTTS
Plasmid#91229Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31YG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_G121VDHFR_WTTS
Plasmid#91233Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationG121VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
FGFR3_HUMAN_D0
Plasmid#79731PurposeThis plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR3 (FGFR3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1 mNFATc2
Plasmid#17858DepositorInsertNFATc2 (Nfatc2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
MK13_HUMAN_D0
Plasmid#79693PurposeThis plasmid encodes the kinase domain of MK13. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertMK13
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDM_noro_S2_lacZA
Plasmid#118816PurposeT7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.DepositorInsertNorovirus toehold switch S2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pH6-mNeon
Plasmid#178460PurposeThe mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.DepositorInsertmNeonGreen
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK357
Plasmid#71568PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-NDFIP2
Plasmid#179330PurposeE. coli optimized expression of GST-fusion of human NDFIP2DepositorInsertNDFIP2 (NDFIP2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS1
Plasmid#145342PurposeCell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFPDepositorInsertZIKV NS1
UseTags8xHis and eGFPExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6272 pDEST-60-GST-HPV16E6
Plasmid#24127DepositorUseTagsExpressionBacterial and MammalianMutationnonePromoterAvailable sinceApril 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-pgaC
Plasmid#154919PurposeaTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymesDepositorInsertgRNA targeting pgaC
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterpTetAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB3aZ_14*
Plasmid#98542Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
KaiB-FLAG
Plasmid#41885DepositorInsertKai B
UseTagsFLAGExpressionBacterialMutationPromotert7 promoterAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pF2
Plasmid#42520DepositorTypeEmpty backboneUseUnspecified; CloningTagsExpressionBacterialMutationPromoterT7, T3Available sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only