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32,684 results

Showing: 11601 - 11650 of 32684 results

  1. pNB3033

    Plasmid
    #113397
    Purpose
    Ty3 Gag3-His tagged, codon optimized for expression in E. coli; . IPTG inducible
    Depositor
    Suzanne Sandmeyer
    Insert
    Ty3 Gag3 codon optimized for E. coli expression
    Tags
    Gag3-6XHis tag
    Expression
    Bacterial
    Available Since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    113397-plasmid-map-sequence-id-217374

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  8. attB Neo* attP

    Plasmid
    #138412
    Purpose
    attB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Att/phic31
    Expression
    Bacterial and Mammalian
    Available Since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138412-plasmid-map-sequence-id-316184

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  15. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

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  17. pGBW-m4046879

    Plasmid
    #149238
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149238-plasmid-map-sequence-id-357573

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  20. J-HARS-4

    Plasmid
    #166529
    Purpose
    scFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166529-plasmid-map-sequence-id-331465

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  21. L-SARS-7

    Plasmid
    #166527
    Purpose
    scFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166527-plasmid-map-sequence-id-329199

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  29. pTU2-a_Sv1KA_amilCP

    Plasmid
    #204054
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var1 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204054-plasmid-map-sequence-id-418176

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  30. pTU2-a_Sv2KA_amilCP

    Plasmid
    #204053
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var2 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204053-plasmid-map-sequence-id-418175

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  31. pTU2-a_SKA_amilCP

    Plasmid
    #204055
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101 (WT) ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204055-plasmid-map-sequence-id-413881

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  32. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid
    #58711
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Depositor
    Hermann Gaub
    Article
    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
    Insert
    Xyl-DocI
    Tags
    HIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cysteine in Xylanase Doma…
    Promoter
    T7
    Available Since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58711-plasmid-map-sequence-id-90776

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  40. pHN873

    Plasmid
    #178829
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A (AR Human)
    Tags
    MBP, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178829-plasmid-map-sequence-id-351932

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  45. pTXB1-TP4

    Plasmid
    #160087
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160087-plasmid-map-sequence-id-313735

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  47. pet15_rbsk

    Plasmid
    #220227
    Purpose
    Bacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similar
    Depositor
    Bernd Nidetzky
    Insert
    ribokinase (rbsK E.coli)
    Tags
    Histag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220227-plasmid-map-sequence-id-436827

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  48. pET3C-ARL16(CO)-His6

    Plasmid
    #216485
    Purpose
    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor-like 16 (ARL16 Human)
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216485-plasmid-map-sequence-id-426900

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  50. C-BLa 2.0

    Plasmid
    #92060
    Purpose
    The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.
    Depositor
    Dieter Langosch
    Article
    Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836
    Insert
    C-BLa 2.0 fusion protein
    Tags
    Flag (DYKDHDG)
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92060-plasmid-map-sequence-id-181586

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Showing: 11601 - 11650 of 32684 results