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Plasmid#174501Purposebacterial co-expression of human SEPT7 and of human SEPT9_i3DepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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pPPC017.109
Plasmid#171145PurposeExpression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmidDepositorInsertsJ109 scRNA
J1-BBa_J23117-mRFP
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J1-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGBW-m4046940
Plasmid#149261PurposeBacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCellFree_G06_ZIKV NS1
Plasmid#145352PurposeCell-free expression vector for Zika virus NS1 protein with C-term mCherryDepositorInsertZIKV NS1
UseTagsmCherry and 8xHisExpressionBacterialMutationPromoterT7Available sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-PATagRFP-KanMX6
Plasmid#87027PurposeTag S. pombe genes in C terminal with PATagRFPDepositorInsertPATagRFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB (TIREVOL)-PAmCherry1
Plasmid#189718Purposetitratable expression of PAmCherry1 in bacteriaDepositorInsertPAmCherry1
UseTagsHis-T7-EKExpressionBacterialMutationPromoterpBADAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PTK2_Focal-AT
Plasmid#178485PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertPTK2_Focal-AT (PTK2 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALxR1
Plasmid#172564PurposeInducible gene expression, pACYC origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (1-73) (72P)
Plasmid#62083PurposeExpression of human p53 (1-73) (72P) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHIsExpressionBacterialMutationcontains TAD residues 1-73; 72PPromoterT7Available sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWT050a
Plasmid#107899PurposeW2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4DepositorInsertPLacO-sgRNA1, PRha-sgRNA4
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a Hbb
Plasmid#26639DepositorInsertHbb (hbb Borrelia burgdorferi)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Ctdnep1_Cter
Plasmid#196522PurposeEntry vector for Gateway with Ctdnep1_CterDepositorInsertCtdnep1-Cter (CTDNEP1 Human)
UseTagsExpressionBacterialMutationNo transmembrane domainPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Ctdnep1
Plasmid#196520PurposeEntry vector for Gateway with Ctdnep1DepositorInsertCtdnep1-N1 (CTDNEP1 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KEAP1_KELCH
Plasmid#178476PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKEAP1_KELCH (KEAP1 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK32-Flag
Plasmid#107432PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertCPK32 (CPK32 Mustard Weed)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pSHS293
Plasmid#87348PurposeD435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET constructDepositorInsertCas9
UseTagsHis-MBPExpressionBacterialMutationC80S/D435C/C574S/E945CPromoterT7Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
6559 pJC25YDJ1 #7
Plasmid#1245DepositorInsertYdj1 (YDJ1 Budding Yeast)
UseTagshis tagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBS-Squ:mCherry
Plasmid#20163DepositorInsertspaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Gamma crystallin TEV LIC cloning vector (2X-T)
Plasmid#29714DepositorTypeEmpty backboneUseTagsHis6-Gamma crystallin-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
ZMYND8 (PRKCBP1A-c097)
Plasmid#98244PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPRKCBP1A (ZMYND8 Human)
UseTagsHis6-Zb-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux LasI
Plasmid#193628PurposeInducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterLux promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGST-TEV-yVms1(1-417,delta(38-69))
Plasmid#128739PurposeBacterial overexpression and crystallizationDepositorInsertVms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
UseTagsGSTExpressionBacterialMutationresidues 1-417 with deletion of 38-69PromoterT7Available sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
dps-RFP
Plasmid#63843PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertdps, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
katG-RFP
Plasmid#63844PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertkatG, mRFP1 and GFPmut3b
UseTagsmRFP1ExpressionBacterialMutationGene is transcriptionally fused to RFPPromoterAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-parkin 219-465
Plasmid#45972DepositorInsertParkin 219-465 (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 StrepII TEV co-transformation cloning vector (13K-HR)
Plasmid#48318DepositorTypeEmpty backboneUseTagsHis6 and StrepIIExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDIV494
Plasmid#177701PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), SNR52p (Candida s…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_target_GUmut33AtoG
Plasmid#139468PurposeT7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 33DepositorInsertTarget_mutGU33
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET21a - Mtb-TF
Plasmid#111513PurposeExpresses M. tuberculosis Trigger Factor with a C-terminal His tagDepositorInsertTrigger Factor
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe-PI(WT)-Rac1(WT)-mCherry
Plasmid#87550PurposePI(WT): Photo-inhibitable/blue light sensitive Rac1DepositorInsertsUseTagsmCherryExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human_TFAM_NoMTS_L6_pET28a+
Plasmid#60012PurposeExpresses TFAM No MTS L6 mutant in bacterial cellsDepositorInsertHuman TFAM No MTS (TFAM Human)
UseTags6xHisExpressionBacterialMutationMissing 1st 42 aas (Mitochondrial targeting seque…PromoterT7Available sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2x-MT2
Plasmid#32102DepositorInsertMT (2x) (Mt1 Mouse)
UseTagsMBPExpressionBacterialMutationPromoterP-lacAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRF-ButR
Plasmid#49359DepositorInsertButR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationE49KPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317
Plasmid#91917PurposeExpresses LweCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLweCas13a and guide expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJOE6227.1
Plasmid#135078PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.DepositorInsertup- and downstream regions of upp (upp Pseudomonas putida)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRAV12
Plasmid#67713PurposeParental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purificationDepositorInsertTranscription cassette for 3'-tagged RNAs
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 RNA polymeraseAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET28a-PKM2-G415R
Plasmid#122693PurposeExpress His-tagged PKM2 (G415R) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationG415RPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry 14-3-3β
Plasmid#111880PurposeMode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: 14-3-3β fused to C-terminal…ExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-Fgß
Plasmid#101719Purposewildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forcesDepositorInsertddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
UseTagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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RECQL5 (RECQL5A-c011)
Plasmid#98231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRECQL5A (RECQL5 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00270:DEAD 29-203, pfam00271:Helicase_C 251-3…PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -