31,059 results
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Plasmid#165490PurposeXenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activityDepositorInsertGlyco-Venus-bPAC(S27A)-Myc
UseCdna expression, xenopus oocyteTagsMyc and VenusExpressionBacterialPromoterT7Available SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXC3GH
Plasmid#47070PurposeFX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagsDepositorTypeEmpty backboneTags3C protease site (PreScission site), GFP, and dec…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2 RanGAP
Plasmid#13379DepositorAvailable SinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mVenus-FLAG:Kan
Plasmid#211830PurposePlasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.DepositorInsertmVenus-FLAG-Kan
ExpressionBacterialMutationNAAvailable SinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP
Plasmid#180153Purposetd-sfGFP dre reporter with Rosa26 recombineering homology armsDepositorInserttd-sfGFP
UseMouse Targeting; Dre/roxExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLQ-KO-tgt50
Plasmid#126578PurposeCRISPR-Cas9 based efficient genome editing in S. aureusDepositorInsertCas9-Pxyltet-sgRNA-Pspac-donor-tgt50
UseCRISPRExpressionBacterialAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-lpp0.2-H38A
Plasmid#201788Purposemutant of PadR; transcriptional regulator can respond to p-coumaric acidDepositorAvailable SinceJuly 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZE-PpadC-yveF-yveG-egfp
Plasmid#201791Purposethe promoter can be recognized by PadRDepositorInsertPpadC-yveF-yveG
ExpressionBacterialMutationWTAvailable SinceJuly 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET empty polycistronic destination vector (2Z)
Plasmid#29776DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pWJB30
Plasmid#126505PurposeSalmonella typhimurium diguanylate cyclase AdrA cloned as AdrA-8xHis in pBAD30DepositorInsertadrA-8xHis
Tags8xHisExpressionBacterialPromoterPBAD30Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTn7xKS-dTomato
Plasmid#117395PurposeTn7 tagging vector pTn7xKS with dTomato expression scaffoldDepositorInsertdTomato
UseSynthetic BiologyExpressionBacterialPromoterPtacAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levels
Plasmid#204801PurposeBacterial expresison of Enterovirus D68 2A proteaseDepositorInsertD68 2A protease
ExpressionBacterialAvailable SinceDec. 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYZ_3WJrep_N24_switch
Plasmid#132725PurposeT7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N24_switch
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-STX6
Plasmid#31581DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
Tags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-Available SinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJM160
Plasmid#134169PurposeBacterial expression of RB3 stathmin-like domain (SLD)DepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PhdeA-cfp
Plasmid#127940PurposeCFP fluorescent reporter for HdeADepositorInsertPhdeA-cfp
ExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
PARD3_PDZ_3
Plasmid#103959PurposeProtein expression and purification of PAR3 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C3-YAP-deltaC
Plasmid#21126DepositorArticleInsertYes-kinase associated protein (YAP1 Human)
TagsEGFP-C3ExpressionBacterial and MammalianMutation5 Carboxy-terminal amino acids -FLTWL are deletedAvailable SinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 EGFP-Sumo2
Plasmid#13385DepositorAvailable SinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pET51b(+)_SNAP-p24
Plasmid#130718PurposePlasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid proteinDepositorInsertSNAP-Tag-p24
TagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ana GvpC-SpyTag-His in pET28a
Plasmid#85737PurposeExpresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coliDepositorInsertGas vesicle protein C (fused to SpyTag and His-tagged)
TagsHis-tag and SpyTagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available SinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK5
Plasmid#53166PurposeExpresses GST-HA tagged human MEK5 from bacterial expression vectorDepositorAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK47
Plasmid#13324DepositorInsertgyrB
ExpressionBacterialAvailable SinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET29b-Ava3858-3855
Plasmid#45746DepositorInsertAva3858-3855
ExpressionBacterialPromoterT7Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
EHF_pet28a
Plasmid#131660PurposeBacterial expression of ETS gene EHFDepositorAvailable SinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-(pos36)GFP-9xGGS-Cre-6xHis
Plasmid#62938PurposeExpression of (+36)GFP-GGS9-Cre-6xHis in bacterial cellsDepositorInsert(ps36)GFP-9xGGS-Cre
Tags(pos36)GFP and 6xHis tagExpressionBacterialPromoterT7Available SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM319
Plasmid#84201PurposeDisturb the expression of miR319 in riceDepositorInsertmiR319
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
A0A075HNX4
Plasmid#177695Purposeexpresses a fatty alcohol oxidase in E. coliDepositorInsertLong chain alcohol oxidase
Tags10xHisExpressionBacterialPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEX-A-U6-TelgRNA_PuroR
Plasmid#84782PurposeExpresses telomere-specific sgRNA.DepositorInserttelomere-specific sgRNA
UseCRISPRExpressionBacterial and MammalianPromoterU6Available SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
M13cp-dg1 hp
Plasmid#218093PurposeHelper plasmid that allows phagemid production upon complementation with a phagemid expressing pIDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE104
Plasmid#59399PurposeMethyloBrick expression vector, coxB promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPcoxBAvailable SinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTE-10M0X
Plasmid#20318DepositorInsertPimyc-tetR#10
ExpressionBacterialAvailable SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 mGrape3
Plasmid#170265PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3DepositorArticleInsertmomeric Grape 3
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pep…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pScaf-10080.1
Plasmid#111410PurposepScaf phagemid with insert for generating DNA origami scaffold 10080 bases in lengthDepositorInsertpScaf-ACKN
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -