32,156 results
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Plasmid#145627PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-C9ORF80
Plasmid#128418PurposeExpress C9ORF80 in E. coli with a His tag (C-terminal)DepositorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pet28a-ClbB-NRPS-NHis
Plasmid#49217PurposeNRPS module of ClbB containing CAT domains with N-terminal 6His tagDepositorInsertClbB-NRPS
Tags6x His tag and T7 tagExpressionBacterialMutationcontains aa4-1080 of reference sequence NP_754362…PromoterT7Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
araC-pBAD-gusA-pBAV1k
Plasmid#30502DepositorAvailable SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
MTST6
Plasmid#86603PurposeCustom designed synthetic DNA fragment lacking homology to natural sequences for preparation of internal standards for RNA-seq in metatranscriptomics.DepositorInsertMTST6
ExpressionBacterialPromoterT7Available SinceSept. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
ATAD2
Plasmid#38916PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE30-rSyntenin1
Plasmid#89428Purposebacterial expression of rat Syntenin1DepositorInsertSDCBP
TagsHisExpressionBacterialPromoterT5Available SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-MBP-CfaN
Plasmid#126016PurposeBacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)DepositorInsertHis5-Sumo-MBP-CfaN
Tags5xHis-SUMOExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R1079A
Plasmid#83494PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)DepositorInsertLbu_C2c2_R1079A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR1079APromoterT7Available SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD_Ub11TCA
Plasmid#174517PurposeRecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitinDepositorInsertUbiquitin
Tags6xHisExpressionBacterialMutationRecoded with TCA replacing codon 11 of wt ubiquit…PromoteraraBADAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_PgyrA_gfp
Plasmid#212138PurposeCargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
ExpressionBacterialPromoterPgyrAAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXD70DA9-Pct5-DadhABC(A2)
Plasmid#191632PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strainDepositorInsertDopamine dehydroxylase
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Spec-yibDp-Erv1p
Plasmid#212094PurposeExpresses sulfydryl oxidase (Erv1p) after phosphate depletionDepositorAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Spec-EV
Plasmid#212095Purpose(Empty Backbone) pCOLA-Spec Empty VectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHHYTK113
Plasmid#200144PurposeType 4a part for Modular Cloning in YeastDepositorInsertsfGFP1-10
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pITC
Plasmid#192856PurposeCloning and expression of transcription factor gene under control of naringenin inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPfdeARAvailable SinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK048
Plasmid#65155PurposeEncodes Spacer as a Type 234 part to be used in the Dueber YTK systemDepositorInsertSpacer
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL692
Plasmid#70284PurposeTn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942DepositorInsertTransposon Tn5-692
ExpressionBacterialAvailable SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRAU171
Plasmid#86835Purposebacterial expression of anti-CRISPR protein AcrIIA2DepositorInsertacrIIA2
ExpressionBacterialPromoteraraBADAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIB168
Plasmid#90191PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoterDepositorTypeEmpty backboneTagsNoneExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PDZ1
Plasmid#59739Purposeexpresses PDZ1 domain of human ZO-1 in bacteriaDepositorAvailable SinceSept. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMD18-T-pyrF
Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…ExpressionBacterialAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-TEV-pro-conA
Plasmid#159527PurposeExpresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.DepositorArticleInsertCanavalia ensiformis pro-concanavalin A
TagsSix-Histidine tag and tobacco etch virus (TEV) pr…ExpressionBacterialPromoterT5Available SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTE-mcs
Plasmid#20320DepositorInsertmultiple cloning site
ExpressionBacterialAvailable SinceMarch 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQadd2
Plasmid#135662PurposeClostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.DepositorInsertLox511/66 and LoxFas/71
UseClostridial vector, pamβ1 originExpressionBacterialAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBRBB-Ppuf843-1200-PR
Plasmid#50963PurposeExpresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroidesDepositorInsertProteorhodopsin
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterPpuf843-1200Available SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_Sensor_32B_LacZ
Plasmid#75007PurposeToehold switch sensor to detect Zika VirusDepositorInsertZIKV_sensor_32B_LacZ
TagsLacZExpressionBacterialPromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Puro-mNeon-WNK3
Plasmid#222674Purposeendogenously tag WNK3 with N-terminal mNeonDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGL821-8
Plasmid#58224PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
ExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOGG011
Plasmid#113985PurposeVector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertpBBR1
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pASPIre1
Plasmid#154842PurposeDerivative of pSEVA291 carrying the bxb1 gene under the control of a rhamnose-inducible promoter and mCherry flanked by attB/P sitesDepositorInsertBxb1 controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
ExpressionBacterialPromoterPrhaAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
RAB3B (3DZ8)
Plasmid#32879PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertRAB3B (RAB3B Human)
TagsHis tag (with TEV cleavage)ExpressionBacterialMutationamino acids 18-190 onlyAvailable SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-hsGFP
Plasmid#64911PurposeEncodes a Fluorescent Reporter for Hydrogen Sulfide in E. ColiDepositorInserthsGFP
TagsHis TagExpressionBacterialMutationChromophore tyrosine of the protein mutated to TA…PromoteraraBADAvailable SinceMay 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
RAB26
Plasmid#25528PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBT3014
Plasmid#122540PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB24
Plasmid#91959Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneTags10-His and Smt3ExpressionBacterial and YeastPromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only