29,780 results

1. pT7C-HSP90AB1

   Plasmid

   #177659

   Purpose

   Recombinant protein synthesis of human HSP90AB1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSP90AB1 (HSP90AB1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMMB67EH-CFP

   Plasmid

   #90103

   Purpose

   CFP control expression plasmid

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   CFP
   

   Use

   Broad-host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMVV099

   Plasmid

   #215305

   Purpose

   Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PCymRC

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPP089

   Plasmid

   #158796

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTW199b2

   Plasmid

   #136928

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   gIII
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGJK_His-SUMO-LbuCas13a

   Plasmid

   #172488

   Purpose

   Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

   Insert

   bdSUMO-LbuCas13a (codon-optimized)
   

   Use

   Tags

   His6 and bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter and lac operator

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FUS_anion

   Plasmid

   #98656

   Purpose

   Expresses untagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-His-VCre

   Plasmid

   #123131

   Purpose

   Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   VCre recombinase
   

   Use

   Cre/Lox

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   see manuscript

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSG66-KcsA-Kv1.3-sfGFP

    Plasmid

    #102457

    Purpose

    Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

    Depositor

    Richard Murray

    Article

    Guo et al bioRxiv 104455

    Insert

    KcsA-Kv1.3
    

    Use

    Tags

    His tag and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUS116

    Plasmid

    #84578

    Purpose

    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium.

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pETM33_AP2B1_adaptin

    Plasmid

    #178467

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    AP2B1_adaptin (AP2B1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBADHisB-Sumo-mBaoJin

    Plasmid

    #229580

    Purpose

    Expresses mBaoJin in bacterial cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

    Insert

    mBaoJin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET.HaloTag.v7

    Plasmid

    #226197

    Purpose

    bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    HaloTag.v7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptetO

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcloDF-kan

    Plasmid

    #184837

    Purpose

    Backbone that confers Kanamycin resistance, contains cloDF ori

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMG36E

    Plasmid

    #133853

    Purpose

    Expression vector

    Depositor

    Gerard Venema, Jan Kok

    Article

    van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P32

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZE27GFP

    Plasmid

    #75452

    Purpose

    Plasmid for constitutive expression of gfp

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET His6 GST TEV LIC cloning vector (1G)

    Plasmid

    #29655

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a-alpha-synuclein

    Plasmid

    #51486

    Purpose

    Inducible expression of human alpha-synuclein in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge