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32,656 results

Showing: 1601 - 1650 of 32656 results

  3. pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

    Plasmid
    #244844
    Purpose
    Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification
    Depositor
    Jennifer Doudna
    Article
    Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka
    Insert
    iCas12a
    Use
    CRISPR
    Tags
    10xHis-CL7-2xSV40 NLS and 4xSV40 NLS
    Expression
    Bacterial
    Mutation
    F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…
    Promoter
    T7
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244844-plasmid-map-sequence-id-486520

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  5. pSCW01

    Plasmid
    #72300
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72300-plasmid-map-sequence-id-255196

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  12. pCK306

    Plasmid
    #110544
    Purpose
    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    PrhaBAD
    yfp
    rhaS
    Expression
    Bacterial
    Promoter
    N/A, PrhaBAD, and kanR promoter (upstream of kanR…
    Available Since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110544-plasmid-map-sequence-id-212164

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  13. Cys-3HB-SPYcatcher

    Plasmid
    #214838
    Purpose
    This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.
    Insert
    Cys-3HB-SPYcatcher
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214838-plasmid-map-sequence-id-425316

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  15. groSL-GFP

    Plasmid
    #109389
    Purpose
    GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.
    Depositor
    Tom Ellis
    Article
    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463
    Insert
    pgroSL-GFP
    Expression
    Bacterial
    Available Since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109389-plasmid-map-sequence-id-210771

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  16. pRSET OCaMP

    Plasmid
    #224932
    Purpose
    Expresses OCaMP, an orange calcium indicator, in bacteria. Contains T7 promoter and RSET leader sequence peptide (His tag, T7 leader, Xpress epitope)
    Depositor
    Alexander Lohman
    Article
    Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://
    Insert
    OCaMP
    Tags
    6xHis, RSET peptide, and Xpress tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 28, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224932-plasmid-map-sequence-id-442661

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  17. pAWO-SUMO-3C_14M_zTDP2

    Plasmid
    #200512
    Purpose
    Expression of zebrafish TDP2 recombinant protein
    Depositor
    Antony Oliver
    Insert
    TDP2 (tdp2b Zebrafish)
    Tags
    HIS-SUMO
    Expression
    Bacterial
    Mutation
    L136N, A139S, C239T, K240R, K309Q, K314L, T315G, …
    Available Since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200512-plasmid-map-sequence-id-396440

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  30. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    Gαs-LgB113 (GNAS Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134362-plasmid-map-sequence-id-306402

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  35. P22_wtCP_SP141his

    Plasmid
    #122651
    Purpose
    Expression of P22 virus like particle in bacteria
    Depositor
    Trevor Douglas
    Insert
    P22 Coat: WT / SP: 6xHis-SP141
    Expression
    Bacterial
    Mutation
    truncated SP, P120H in the capsid scaffold (see …
    Available Since
    Aug. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    122651-plasmid-map-sequence-id-237561

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  41. 6xHis-MBP-huTsCas12a

    Plasmid
    #174687
    Purpose
    Expresses 6xHis-MBP tagged TsCas12a
    Depositor
    Piyush Jain
    Article
    Nguyen et al medRxiv 2021.07.21
    Insert
    huTsCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174687-plasmid-map-sequence-id-346204

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  49. EIF5A

    Plasmid
    #25260
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    eIF5A:S15-A151 (EIF5A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S15-A151
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1601 - 1650 of 32656 results