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31,059 results

Showing: 2501 - 2550 of 31059 results

  6. p406TDH3

    Plasmid
    #15977
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15977-plasmid-map-sequence-id-8462

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  16. pJK651

    Plasmid
    #71702
    Purpose
    Produces E. coli thioredoxin reductase with a C-terminal His6 tag (EcTrxBH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71702-plasmid-map-sequence-id-131727

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  20. pTCF236 HLA-B*40:01

    Plasmid
    #180463
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*40:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180463-plasmid-map-sequence-id-355082

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  37. pUC19-T7-3WJdB-T

    Plasmid
    #87308
    Purpose
    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Three-way junction dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87308-plasmid-map-sequence-id-174739

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  38. pNR220

    Plasmid
    #79541
    Purpose
    Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    A118
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79541-plasmid-map-sequence-id-148753

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  39. pRS316-RGR-GFP

    Plasmid
    #51056
    Purpose
    Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    RGR-GFP
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    Yeast ADH1 promoter
    Available Since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51056-plasmid-map-sequence-id-77326

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  43. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

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  45. ELONGIN BC (XX01TCEB1A-c001)

    Plasmid
    #110274
    Purpose
    Elongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin C
    Depositor
    Nicola Burgess-Brown
    Insert
    Elongin BC (ELOC, ELOB Human)
    Expression
    Bacterial
    Mutation
    Elongin B is full length. Elongin C contains a.a…
    Promoter
    T7
    Available Since
    May 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    110274-plasmid-map-sequence-id-209817

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  46. pBS mTSP-2

    Plasmid
    #12999
    Depositor
    Jack Lawler
    Insert
    TSP-2 (Thbs2 Mouse)
    Expression
    Bacterial
    Available Since
    Sept. 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  47. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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Showing: 2501 - 2550 of 31059 results