32,069 results
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Plasmid#32640DepositorInsertTetanus toxin light chain
ExpressionBacterialAvailable SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-CspGFP
Plasmid#40730DepositorInsertLeucine Zipper prime - C super positive Green Fluorescent Protein
ExpressionBacterialPromoterpBadAvailable SinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-R399E
Plasmid#122691PurposeExpress His-tagged PKM2 (R399E) protein in bacteria.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
Plasmid#80840PurposeModified helper phageDepositorInsertsM13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV
ExpressionBacterialAvailable SinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
IBMc396
Plasmid#161954PurposeDemonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHHDepositorInsertpSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
ExpressionBacterialPromoterPBADAvailable SinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-LARP7
Plasmid#113545PurposeProduces an N-terminal GST-tagged full length LARP7 protein.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H2B.1
Plasmid#42630DepositorAvailable SinceFeb. 22, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18T-mini-Tn7T-Gm-Pc-mCherry
Plasmid#199247PurposeFluorescent labelling of bacterial cells with mCherryDepositorInsertCodon-optimized mCherry gene
ExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-huH2Ax-huH2B
Plasmid#197273PurposeHuman (hu) histones H2Ax and H2B inserted into a plasmidDepositorInsertshuH2Ax
huH2B
TagsHistidine tag and No tagExpressionBacterialAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL97
Plasmid#78576PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoterDepositorTypeEmpty backboneUseStreptomyces expressionExpressionBacterialPromoterermEp* promoter from S. erythraeaAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPP085
Plasmid#158795PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-2
Tags6xHisExpressionBacterialAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HP17 sfGFP::P8
Plasmid#235448PurposeHelper plasmid with sfGFP gene inserted at gene VIII locusDepositorInsertCoding part of the M13KO7 helper phage genome, except gene VIII
ExpressionBacterialAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEM7_CL7_Bac_2NLS-Flex-Cas12a (LbCas12a)-4NLS
Plasmid#244842PurposeBacterial expression of Flex-Cas12a LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminusDepositorInsertFlex-Cas12a
UseCRISPRTags10xHis-CL7-2xSV40 NLS and 4xSV40 NLSExpressionBacterialMutationG146R/R182V/D535G/S551F/D665N/E795QPromoterT7Available SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHK326
Plasmid#235475PurposeInducible gene VIII by Ptac promoterDepositorInsertphage M13 geneVIII
ExpressionBacterialAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 IntS12
Plasmid#210521PurposeExpresses GST-tagged Drosophila IntS12DepositorAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Himar-dCas9-mammalian
Plasmid#138414PurposeProtein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tagDepositorInsertHimar-dCas9
Tags3xFLAG, 6xHis, and SV40 NLSExpressionBacterialPromoterT7Available SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCLASP_mScarlet_CLASP_RGS2membrane
Plasmid#133086PurposePlasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to blDepositorInsertmScarlet-CLASP
ExpressionBacterial and YeastAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGEX3X-GST-PTEN
Plasmid#20743DepositorAvailable SinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Twin-Strep(R)-Tev-GSGS-Ubiquitin
Plasmid#186803PurposeCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.DepositorInsertTwin-Strep(R)-Tev-GSGS-Ubiquitin
TagsGS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…ExpressionBacterialMutationStrep tag Lysine's mutated to ArginineAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET11a-4SS-HEWL
Plasmid#39233DepositorInsertHen egg white lysozyme (LYZ Chicken)
ExpressionBacterialMutationN-terminal MetPromoterT7Available SinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJ2072.2_CRISPRlator
Plasmid#140674PurposeCRISPRlatorDepositorInsertCRISPRlator
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Pa1
Plasmid#138475PurposeEnables high-efficiency recombineering in P. aeruginosaDepositorInsertsPapRecT
mutL E36K
ExpressionBacterialMutationE36KAvailable SinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pThermeleon
Plasmid#86120PurposeBandpass temperature-gated expression of mWasabi and mCherry reportersDepositorInsertsTlpA
mWasabi
TcI38
mCherry
cI
Tags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…PromoterpR/pL, pR/pL, pLacI, and pTlpAAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_mScarlet
Plasmid#136967PurposeGoldengate/Greengate CDS module, containing mScarletDepositorInsertmScarlet
ExpressionBacterialAvailable SinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet-I3-SYFP2
Plasmid#189763PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet-I3-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET MBP His6 LIC cloning vector (2Cc-T)
Plasmid#37237DepositorTypeEmpty backboneTagsHis6 and MBPExpressionBacterialPromoterT7Available SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-NspGFP
Plasmid#40729DepositorInsertN super positive GFP - Leucine Zipper
TagsHisExpressionBacterialPromoterT7Available SinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI
Plasmid#184742Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
ExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
TTC5
Plasmid#39064PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2m
Plasmid#176844PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-Sec-APEX2m
UseInducibleExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialAvailable SinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL52 (clone76)
Plasmid#188579PurposeHigh-yield production of coenzyme F420 in E. coliDepositorInsertsribA
ribD
yigB
fbiC
cofC
cofD
cofI
cofE
ExpressionBacterialMutationCodon optimization, synthetic ribosome binding si…PromoterT7 variantsAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-vp16
Plasmid#171075PurposeExpresses Gal4-vp16 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
TagsHis and VP16 AD (413-490)ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_rPD-L1nb
Plasmid#226715PurposeHeterologous expression and purification of recombinant PD-L1 nanobody (rPD-L1nb)DepositorInsertRecombinant PD-L1 nanobody
TagsHA tag and Histidine tagExpressionBacterialPromoterT7Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEOS3.2-KanMX6
Plasmid#87030PurposeTag S. pombe genes in C terminal with mEOS3.2DepositorInsertmEOS3.2
ExpressionBacterial and YeastAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427T
Plasmid#81035PurposeMammalian expression of LOV I427T, a mutant with super short relaxation timeDepositorInsertLOV I427T
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427TAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30ax
Plasmid#85761PurposeA bacterial expression vector with a His-tag at N and C-terminus.DepositorTypeEmpty backboneTagsHis tag (N-term and C-term)ExpressionBacterialPromoterT7 promoterAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGR-BBa_B0010
Plasmid#46003DepositorInsertBBa_B0010 Terminator
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBAD-GreenPy1Highest
Plasmid#239049PurposeBacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1HighestDepositorInsertGreenPy1Highest
ExpressionBacterialAvailable SinceJune 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP1D1
Plasmid#199768PurposeExpresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1D1
TagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiAvailable SinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only