29,729 results

1. pDNA2.0-6H-TEV-KRAS G12D

   Plasmid

   #159439

   Purpose

   Expresses KRAS G12D in E.coli

   Depositor

   Kenneth Westover

   Article

   Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

   Insert

   KRAS G12D (KRAS Human)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pE1n

   Plasmid

   #17441

   Purpose

   modified pENTR vector for N-terminal STREP tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   STREP

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBFC1207

   Plasmid

   #186694

   Purpose

   VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   Vc_2xBsaI_NT (Guide Stuffer) crRNA
   Tn7 transposon
   GmR+sfGFP VcDART Cargo
   SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DYRK1A

   Plasmid

   #38913

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DYRK1A (DYRK1A Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pR_Lux

   Plasmid

   #226457

   Purpose

   lambda immunity region to the luciferase operon (lux)

   Depositor

   Emily Balskus

   Article

   Silpe et al Nature. 2022 Mar;603(7900):315-320. doi:

   Insert

   lambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQLinkN

   Plasmid

   #13670

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SurA

   Plasmid

   #213138

   Purpose

   Expresses chaperone SurA

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   SurA (21-428) (surA E.coli)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMAK461

   Plasmid

   #140699

   Purpose

   NBαRb-IgG-Fc

   Depositor

   Takayuki Iwaki

   Article

   Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

   Insert

   NBαRb-IgG-Fc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSG28-J23151-AraC

   Plasmid

   #102453

   Purpose

   Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   AraC (araC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23151

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. enz.083-NMT1

    Plasmid

    #154047

    Purpose

    Expresses NMT1 in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    NMT1 (N-myristoyltransferase 1) (NMT1 Human)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Map3k1

    Plasmid

    #29581

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Map3k1 (MAP3K1 Human)
    

    Use

    Tags

    GST, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    Constitutively Active. Contains catalytic domain …

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pFW2000

    Plasmid

    #224211

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GEF-H1 FLARE191.pa

    Plasmid

    #134931

    Purpose

    GEF-H1 biosensor that has GEF activity.

    Depositor

    Klaus Hahn

    Article

    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

    Insert

    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    

    Use

    Tags

    None.

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-HIV p24

    Plasmid

    #212195

    Purpose

    Bacterial expression plasmid for production of HIV p24 protein with a polyhistidine tag at the N-terminus

    Depositor

    Richard C. Willson

    Insert

    Chain A, Capsid protein p24, HIV type 1
    

    Use

    Tags

    Polyhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a_T7-ARG2

    Plasmid

    #106474

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MDH2

    Plasmid

    #38792

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MDH2 (MDH2 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease

    Plasmid

    #228637

    Purpose

    Bacterial expression of the Coxsackievirus 16 3C protease

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Coxsackievirus 16 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pNMTMA_MBP-Cav1-His6

    Plasmid

    #223700

    Purpose

    To produce standard caveospheres (without the Z-domain), Parton Lab clone LDB

    Depositor

    Rob Parton

    Article

    T. D. et al bioRxiv

    Insert

    MBP-Cav1-His6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMRE-Tn5-145

    Plasmid

    #118529

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mScarlet-I
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSW068

    Plasmid

    #140036

    Purpose

    KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cLac143

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAJM.847

    Plasmid

    #108524

    Purpose

    PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PhlFAM + PPhlF-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEXP5-NT/pLas eGFP

    Plasmid

    #193630

    Purpose

    Inducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    eGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LbCas12a-gRNA_BbsI_CAM

    Plasmid

    #183222

    Purpose

    LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    LbCas12a guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDJSVT87

    Plasmid

    #109302

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Nme2Cas9_pMCSG7

    Plasmid

    #119925

    Purpose

    Nme2Cas9 bacterial expression plasmid

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    Tags

    NLS, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVRa41_491

    Plasmid

    #49702

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF41_491
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p3187 HPV-16 E2

    Plasmid

    #10846

    Depositor

    Peter Howley

    Article

    Yasugi et al J Virol. 1997 Feb . 71(2):891-9.

    Insert

    HPV-16 E2 (E2 Human papillomavirus)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. hsp70: mKate2-DN Kif5a

    Plasmid

    #105972

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Kif5a (kif5aa Zebrafish)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    truncation of aa416-1033

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPEP-TEV cNup98 (1-498)

    Plasmid

    #38037

    Depositor

    Roderick Lim

    Article

    Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

    Insert

    Nucleoporin 98 (NUP98 Human)
    

    Use

    Tags

    His-tag, 3Cysteine

    Expression

    Bacterial

    Mutation

    amino acids 1-498

    Promoter

    T7

    Available since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPBO.ACT006

    Plasmid

    #182716

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    GFP in cloning site of crRNA for easier screening…

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SuperClomeleon

    Plasmid

    #203724

    Purpose

    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

    Depositor

    George Augustine

    Article

    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

    Insert

    Cerulean linked topaz fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Cerulean, modified Topaz Fluorescent Protein with…

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET20b-NLuc-MBP-6xHis

    Plasmid

    #135931

    Purpose

    Expresses NanoLuciferase in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    NanoLuciferase
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBA562

    Plasmid

    #186237

    Purpose

    pTet-RfxCas13d crRNA entry vector

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid

    #129628

    Purpose

    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    FHL-cpmTq2-Calcium-lifetime-sensor
    

    Use

    Tags

    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV,&Rhamnose

    Available since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AK1

    Plasmid

    #39134

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AK1 (AK1 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pTC-0X1L

    Plasmid

    #20315

    Depositor

    Dirk Schnappinger

    Article

    Klotzsche et al Nucleic Acids Res. 2009 Jan 27. ():.

    Insert

    Pmyc1tetO-lacZ (lacZ E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pACYC-T7

    Plasmid

    #41187

    Depositor

    Dan Bolon

    Article

    Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

    Insert

    S. cerevisiae Hsp82
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pVRa42_4454

    Plasmid

    #49703

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF42_4454
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pH3U3-mcs

    Plasmid

    #12609

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Multiple cloning site in His3 Ura3 reporter plasmid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEX-5X-3-SARS-CoV-2-3CL

    Plasmid

    #168457

    Purpose

    Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

    Depositor

    David Ho, Alejandro Chavez, Sho Iketani

    Article

    Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

    Insert

    SARS-CoV-2 3CL protease (ORF1ab Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

44. pgRNAtet-lvaA

    Plasmid

    #106397

    Purpose

    Guide RNA plasmid targeting lvaA on BBR1-UP origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    sgRNA towards lvaA in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pFDH

    Plasmid

    #131706

    Purpose

    Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

    Depositor

    Ron Milo

    Article

    Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

    Insert

    formate dehydrogenase
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET-Duet-1-NONO(53-312)

    Plasmid

    #135442

    Purpose

    Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Non-POU domain-containing octamer-binding protein (NONO Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBJ3

    Plasmid

    #167132

    Purpose

    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Frr promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pOPARA2

    Plasmid

    #200417

    Purpose

    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)

    Depositor

    Kamil Witek

    Article

    Lesley et al bioRxiv

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET28a-RBP-LOV2

    Plasmid

    #185792

    Purpose

    Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

    Depositor

    Lizhong He

    Article

    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

    Insert

    RBP-LOV2
    

    Use

    Tags

    10xHis, TEV protease cleavage site after His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMRE140

    Plasmid

    #118492

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge