4,932 results

1. pFastBac Dual His6 TEV LIC cloning vector (5B)

   Plasmid

   #30122

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6-TEV

   Expression

   Insect

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB1.HMBP.PrS.EZH2

   Plasmid

   #125161

   Purpose

   Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Baculovirus expression vector

   Tags

   His-MBP

   Expression

   Insect

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pE2n

   Plasmid

   #17456

   Purpose

   modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKRG3-mU6-PUb-hSpCas9

   Plasmid

   #162162

   Purpose

   Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   N/A

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAW-Nb127D01-GFP

   Plasmid

   #171571

   Purpose

   Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-GFP (CXCR2 Human)
   

   Tags

   GFP

   Expression

   Insect

   Promoter

   fly actin5C promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

   Plasmid

   #86453

   Purpose

   UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)

   Depositor

   Gregor Bucher

   Insert

   3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
   

   Expression

   Insect

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-His-Tipin

   Plasmid

   #22891

   Depositor

   Aziz Sancar

   Article

   Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

   Insert

   Tipin (TIPIN Human)
   

   Tags

   His

   Expression

   Insect

   Available Since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pSL1180-HR-PUbECFP

   Plasmid

   #47917

   Purpose

   Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

   Depositor

   Leslie Vosshall

   Insert

   ECFP
   

   Tags

   NLS

   Expression

   Insect

   Promoter

   Aedes aegypti polyubiquitin (PUb)

   Available Since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNDamMyc

   Plasmid

   #59217

   Purpose

   Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Type

   Empty backbone

   Use

   Damid

   Tags

   Dam and Myc

   Expression

   Insect

   Promoter

   Drosophila heat-shock promoter

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBFv-nosP-Cas9

    Plasmid

    #138402

    Purpose

    Expresses Cas9 in the germline of Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Insert

    Cas9-NLS
    

    Expression

    Insect

    Promoter

    UAS

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Rab5

    Plasmid

    #31733

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab5 (Rab5 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

    Plasmid

    #55222

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    SNAPf and TwinStrepII

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP284

    Plasmid

    #113312

    Purpose

    human Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

    Insert

    Nbs1 (NBN Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1 human full-length CENP-E-mNeon-6xHis

    Plasmid

    #180484

    Purpose

    pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

    Depositor

    Julie Welburn

    Article

    Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

    Insert

    CENP-E (CENPE Human, Synthetic, human)
    

    Tags

    mNeon-6xHis

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB.BIG1a.PRC2

    Plasmid

    #234581

    Purpose

    Expression of EZH2, MBP-SUZ12, EED, RBBP4

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
    

    Expression

    Insect

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMB.PRC2.AEBP2

    Plasmid

    #234580

    Purpose

    Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4 and AEBP2 [Isoform 3]

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    EZH2, SUZ12, EED, RBBP4, AEBP2 [Iso 3] (EZH2, SUZ12, RBBP4, EED, AEBP2 Human)
    

    Expression

    Insect

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB.PRC2

    Plasmid

    #234579

    Purpose

    Expression of MBP-EZH2, MBP-SUZ12, MBP-EED and MBP-RBBP4

    Depositor

    Chen Davidovich

    Article

    Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

    Insert

    EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)
    

    Expression

    Insect

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-Rab9

    Plasmid

    #31736

    Depositor

    Ron Vale

    Article

    Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

    Insert

    Rab9 (Rab9 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Actin

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pFastBac1_FLAG-BPTF

    Plasmid

    #102242

    Purpose

    Expression of human Flag-BPTF using baculovirus from pFastBac1

    Depositor

    Joe Landry

    Insert

    Flag-BPTF (BPTF Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Polyhedron

    Available Since

    Dec. 11, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pUASp-GBP-Tbro

    Plasmid

    #179995

    Purpose

    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

    Depositor

    Graydon Gonsalvez

    Article

    Baker et al Development. 2021 Nov 15;148(22). pii: 2

    Insert

    GBP nanobody
    

    Tags

    Trbo

    Expression

    Insect

    Promoter

    UAS-Gal4

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKM19

    Plasmid

    #123655

    Purpose

    Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Expression

    Insect

    Promoter

    pIEx

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR R4-vas2-integrase-R3

    Plasmid

    #62299

    Purpose

    phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    phageC31 integrase under control of vasa promoter and terminator
    

    Tags

    HA and SV40 NLS

    Expression

    Insect

    Promoter

    vasa

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac_Dual_SNF2L-opt_p48

    Plasmid

    #102243

    Purpose

    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual

    Depositor

    Joe Landry

    Inserts

    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    

    Expression

    Insect

    Mutation

    codon optimized for expression

    Promoter

    Polyhedrin and p10

    Available Since

    Nov. 1, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJFRC177-10XUAS-FRT>-dSTOP-FRT>-myr::GFP

    Plasmid

    #32149

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    FRT> STOP STOP FRT>
    

    Expression

    Insect

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFastBac Dual LIC cloning vector (5A)

    Plasmid

    #30121

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTriEx-mCherry-LOV2 V416L

    Plasmid

    #81036

    Purpose

    mammalian expression of LOV V416L, a mutant with super long relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416L
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416L

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCFD5-frame_selector_0,1,2

    Plasmid

    #131152

    Purpose

    Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    CRISPaint frame selector sgRNAs 0,1,2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6:3

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFB-EPCP1

    Plasmid

    #226783

    Purpose

    Expression of Z-tagged LRRK2 RCKW using Baculoviruses

    Depositor

    Sebastian Mathea

    Article

    Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Amino acids deleted 1-1326

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pmec-4::Chrimson::SL2::mCherry::unc-54

    Plasmid

    #107745

    Purpose

    Express Chrimson and mCherry in mechanosensory neurons.

    Depositor

    Andrew Leifer

    Article

    Liu et al Elife. 2018 Jun 26;7. pii: 36419. doi: 1

    Inserts

    Chrimson
    mCherry
    

    Expression

    Insect and Worm

    Promoter

    mec-4

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 891 pFastBac GST PTEN

    Plasmid

    #10937

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

33. pSLfa[UAS-Tc'hsp_p-tGFP-SV40]fa

    Plasmid

    #86452

    Purpose

    UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Shuttle plasmid); tGFP can be exchanged by other ORFs

    Depositor

    Gregor Bucher

    Insert

    UAS-Tc'hsp_p-tGFP-SV40
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pExpreS2-2

    Plasmid

    #175362

    Purpose

    G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

    Depositor

    ExpreS2ion Biotechnologies

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p1177-X1-8

    Plasmid

    #38150

    Depositor

    David Waugh

    Article

    Austin et al Protein Expr Purif. 2011 May;77(1):53-61

    Insert

    Carboxypeptidase A
    

    Tags

    HHHHHHRR

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJC-20xUAS-TALE3-VP64-P10

    Plasmid

    #104607

    Purpose

    Expresses TALE3 under UAS control

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    TALE3
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p{CFD4-3xP3::DsRed}

    Plasmid

    #86864

    Purpose

    Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFAST-BAC BAF155/SMARCC1-His

    Plasmid

    #177863

    Purpose

    Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

    Depositor

    Yoshihiro Izumiya

    Article

    Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

    Insert

    SMARCC1 (SMARCC1 Human)
    

    Tags

    His and His tag at C-terminus with GGGGS linker

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTiger-OptoSOS

    Plasmid

    #86439

    Purpose

    Single-construct blue light optogenetic Ras activator for use in Drosophila

    Depositor

    Jared Toettcher

    Article

    Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

    Insert

    OptoSOS
    

    Tags

    tagRFP

    Expression

    Insect

    Promoter

    Gal4-UAS

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJC-20xUAS-TALE4-VP64-P10

    Plasmid

    #104608

    Purpose

    Expresses TALE4 under UAS control

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    TALE4
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge