4,932 results
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Plasmid#26217DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)
Plasmid#48297DepositorTypeEmpty backboneTagsStrepII and msfGFPExpressionInsectAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dll4ICD-HPC4 pBacPAK8.3
Plasmid#17335DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUb RxCas13d + RxCas13d guide RNA
Plasmid#176303PurposeExpresses RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
TagsHA tagExpressionInsectPromoterUbi-p63eAvailable SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
Tags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC120-MBP-DDX3X-monoGFP-His
Plasmid#219985PurposeDDX3X(WT) protein purificationDepositorAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
OA-1053A
Plasmid#184006PurposeExpresses arrays of gRNA targeting eve promoter under U6b/c promoterDepositorInsertU6b:sgRNAeve1 - U6c:sgRNAeve2
ExpressionInsectAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
Plasmid#159676PurposePlasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.DepositorInsertpBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
ExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
TagsFLAG purification tag and eGFPExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSlmb-vhhGFP4
Plasmid#35577DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionInsectAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
p130Cas
Plasmid#186131PurposeFor expression of recombinant p130Cas in SF9 cells.DepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab5 WT
Plasmid#58446Purposeexpression of drosophila td tomato Rab5DepositorAvailable SinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPINN-GFP
Plasmid#53541PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
TagsN-His-GFPExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.AEBP2
Plasmid#125165PurposeExpresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2
Plasmid#85072PurposeGreen/red FRET biosensor for RhoA in the GTP locked constitutively active stateDepositorInsertRhoA Biosensor constitutively active (Q63L) (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCGMbX
Plasmid#42300DepositorTypeEmpty backboneTagsBiP signal sequence, His6, and V5-epitopeExpressionInsectPromotermetallothoneinAvailable SinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEBFP2-NLS
Plasmid#176682PurposeExpression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEBFP2
ExpressionInsectPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Dual-StrepII-BARD1
Plasmid#137166PurposeExpresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell systemDepositorInsertBARD1 (BARD1 Human)
TagsStrepIIExpressionInsectMutationfully synthetic, codon optimized for insect expre…Promoterp10Available SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-gEgIL
Plasmid#11622DepositorInsertHSV-1 glycoprotein E and HSV-1 glycoprotein I
TagsHisExpressionInsectMutationDicistronic vector containing HSV-1 gE (KOS strai…Available SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFastbac1-GST-hUSP7
Plasmid#63573PurposeExpression of synthetic human USP7 tagged with GSTDepositorInsertUSP7 (USP7 Human, Synthetic)
TagsGSTExpressionInsectMutationSynthetic based on human protein sequence (please…PromoterPolyhedrinAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB-LIC-Bse
Plasmid#26108PurposeSGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selectionDepositorTypeEmpty backboneUseBaculovirus expressionTagsHis6 - TEVExpressionInsectPromoterpolyhedrinAvailable SinceOct. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac1-Ls-AChBP
Plasmid#63126PurposeExpression of Lymnaea stagnalis AChBPDepositorInsertAChBP
ExpressionInsectPromoterPolyhedrinAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSAR-Cre-F2A-I-SceI
Plasmid#62302PurposeAnopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed markerDepositorInsertCre recombinase, I-SceI nuclease
ExpressionInsectPromotervasaAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-MCS
Plasmid#52908PurposeAedes aegypti polyubiquitin promoter followed by MCSDepositorTypeEmpty backboneExpressionInsectPromoterAe aegptyi polyubiquitinAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-GFP(39TAG)
Plasmid#217360PurposeExpression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmidDepositorInsertEnhanced green fluorescent protein
UseBaculoviralExpressionInsect and MammalianMutationY39TAGPromoterCMVAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKF295
Plasmid#72871Purposeexpression of improved FLP recombinaseDepositorAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNAy
Plasmid#168294PurposeExpresses gRNA targeting the yellow gene in DrosophilaDepositorInsertU6.3-gRNA[y]-scaffold
ExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastbacHTB HA-human PP2A Cα D88N
Plasmid#132633Purposeexpresses HA-human PP2A Cα D88N in Hi5 insect cellDepositorInsertPP2A Cα D88N
ExpressionInsectAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINVL
Plasmid#26040DepositorInsertu-LacZ
Tagsu-phosphatase secretion leaderExpressionBacterial, Insect, and Mamm…Available SinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10
Plasmid#162385PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
Tags6xHisExpressionInsectMutationQ315LPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJFRC3-1XUAS-IVS-mCD8::GFP
Plasmid#26216DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7
Plasmid#214140PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7DepositorInsertsTags2xSTIIExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
ExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-YFPsv40 GGGG AAGA
Plasmid#174530Purpose3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-YFP BsaI cassette
ExpressionInsectPromoter3xP3Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_WT
Plasmid#162579PurposeExpresses norovirus GI.1 VP1 protein in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectPromoterpolyhedrinAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
908H
Plasmid#160240PurposeTetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attBDepositorInsertsTet Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
TagsT2A GFPExpressionInsectAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_ybbR_Tau MTBD
Plasmid#159719PurposeExpresses microtubule-binding domain of tau (amino acids 242-367) in Sf9 cells with a ZZ affinity tag and a ybbR tagDepositorInsertTau microtubule-binding domain only (amino acids 242-367) (MAPT Human)
TagsZZ affinity tag, ybbR tagExpressionInsectMutationOnly amino acids 242-367Available SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only