5,038 results
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Plasmid#83957PurposeExpresses conditional RU486-dependent GAL4 protein under control of the elav promotorDepositorInsertExpressionInsectPromoterelavAvailable SinceDec. 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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UAS-Syt::7xHalo7
Plasmid#87650PurposeFor creating drosophila transgenics expressing UAS-Syt::7xHalo7DepositorInsertSyt::7xHalo7
ExpressionInsectMutationS265G in Syt (please see depositor's commen…Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSquid_AA
Plasmid#148229PurposeInsect Expression of DmSquidDepositorInsertDmSquid (sqd Fly)
ExpressionInsectAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB1_hMLH1
Plasmid#129426PurposeExpression human MLH1 protein using baculovirusDepositorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP
Plasmid#165830PurposeEGFP green fluorescent protein CDSDepositorInsertEGFP
ExpressionInsectAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnos-Cas9-nos
Plasmid#62208PurposeExpresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.DepositorInsertcas9
ExpressionInsectPromoternanosAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVL1393-6xMyc
Plasmid#128035PurposeBaculovirus expression vector with C-terminal 6xMyc tagDepositorTypeEmpty backboneTags6xMyc tagExpressionInsectAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMycDam
Plasmid#59218PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli DamDepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectPromoterDrosophila heat-shock promoterAvailable SinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-QtgRNA2.1-BR
Plasmid#170513PurposegRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST-2x attB
Plasmid#206362Purposeincludes two standard UAS cassettes and a third cassette without a promoterDepositorTypeEmpty backboneExpressionInsectAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FoRC.attBGm
Plasmid#84588PurposeDrosophila PhiC31 transgenesis vector for cloning rescue transgenes. Cloning site is flanked by FRT sequences and contains constitutive GFP reporter.DepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
ExpressionInsectPromoterp10Available SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
No-repeats
Plasmid#122487PurposeA control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)DepositorInsertLacZ
ExpressionInsectAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e
Plasmid#80615PurposepBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSSRB070_pAC8-hsCRBN
Plasmid#218789PurposeInsect cell expression vector for FLAG-TEV-Spy-hsCRBNDepositorAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTa_LYN
Plasmid#224244PurposeExpression of human LYN Kinase in insect cells (SF9 cells)DepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
Tags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-DYNC2H1
Plasmid#132524PurposeFull length human dynein 2 heavy chain DYNC2H1, Sf9 codon optimised. With 8xHis-ZZ tag, TEV cleavage site, SNAPf tag, precission cleavage site at N-terminus. Assembled from synthesised fragmentsDepositorInsertDYNC2H1 (DYNC2H1 Human)
Tags8x His, Linker-TEV-linker-TEV, Precission cleavag…ExpressionInsectMutationCodon optimised for Sf9 expressionAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACU2_CD4-mIFP T2A HO1
Plasmid#72441PurposeExpressing UAS-CD4-mIFP T2A HO1 in drosophilaDepositorInsertCD4-mIFP T2A HO1
ExpressionInsectAvailable SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
46 pLIB_3C_C_HIS
Plasmid#160188PurposeC-term 3C cleavable His tag for insect cell expressionDepositorTypeEmpty backboneTags12xHisExpressionInsectPromoterpolyhydrinAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 TEV LIC cloning vector (4B)
Plasmid#30115DepositorTypeEmpty backboneTagsHis6-TEVExpressionInsectAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7
Plasmid#214140PurposeInsect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7DepositorInsertsTags2xSTIIExpressionInsectPromoterpolyhedrinAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
15x-QUAS-dTomato-T2A-GCaMP6s
Plasmid#130666PurposeQUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2ADepositorInsert15xQUAS-dTomato-T2A-GCaMP6s
UseAe. aegypti expressionExpressionInsectPromoter15xQUAS-TATAAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-mScarlet-I
Plasmid#138391PurposeRMCE vector for generating C-terminal mScarlet-I fusionDepositorInsertmScarlet-I
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOT563
Plasmid#40864DepositorInsertOxytocin promoter
UseAAVExpressionBacterial and InsectPromotermouse oxytocin promoterAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-LexA
Plasmid#26258DepositorInsertLexA
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
p4X-UASTattB
Plasmid#189858PurposeFly cloning and transformationDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
DDB1
Plasmid#25246PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDDB1_1 (DDB1 Human)
UseBaculovirus expressionTagsHisExpressionInsectPromoterpolyhedrinAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac Dual wt p110α/p85α
Plasmid#199302Purposeco-expression of His-tagged p110α wt and Avi-tagged p85α wt proteinsDepositorTagsAvi and HisExpressionInsectAvailable SinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10
Plasmid#162383PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8s
Tags6xHisExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectPromoterPolyhedrinAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFlpStop-HDR-UAS-2.1-tdTom
Plasmid#89148PurposeFlpStop plasmid for CRISPR/HDR. Has MCS for homology armsDepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoter5XUAS, 3XP3-dsRedAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-Lckg2a)
Plasmid#177891PurposeBaculo expression of His10 tag fused to human LckG2ADepositorAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TetR in pENTR1A (559-1)
Plasmid#22265DepositorInsertTetracycline repressor (tetR )
ExpressionInsect, Mammalian, Worm Exp…Available SinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL4.2Uw-2
Plasmid#26227DepositorInsertGAL4 (GAL4 Budding Yeast)
ExpressionInsectAvailable SinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOPINF
Plasmid#26042DepositorInsertHis3C-lacZ
TagsMAHHHHHHSSGLEVLExpressionBacterial, Insect, and Mamm…Available SinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOCC167
Plasmid#118909Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C proteaseDepositorInsertNcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHSV-TK
Plasmid#165801PurposeMinimal viral terminator derived from Herpes Simplex 1 thymidine kinase geneDepositorInsertHSV-TK
ExpressionInsectAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBDM
Plasmid#110738PurposePlasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transpositionDepositorTypeEmpty backboneExpressionInsectPromoterPolyhedrin and p10 promotersAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pActP-GCaMP6m
Plasmid#64714Purposevisualizing calcium in cultured Drosophila S2 cellsDepositorInsertGCaMP6m
ExpressionInsectAvailable SinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFast-hHus1
Plasmid#222469PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
ExpressionInsectAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSSRB274_pAC8-eGFP-hsCRBN
Plasmid#218791PurposeInsect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
TagsFLAG-TEV-eGFP-PrescissionExpressionInsectMutationeGFP fused to N-terminusPromoterpolyhedrinAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
VT028327-FRT10-STOP-FRT10-GAL4
Plasmid#232829PurposeEnhancer VT028327 driven GAL4, gated by Sparse.FRT10DepositorInsertsExpressionInsectAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
tubP-FRT>GAL80-6-FRT>
Plasmid#63173Purposeubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassetteDepositorInsert"Flp-Out" GAL80
ExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-GAL80
Plasmid#46137DepositorInsertGAL80 (GAL80 Budding Yeast)
ExpressionInsectAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10
Plasmid#218862PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8m
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBPhsFlp1
Plasmid#32148PurposeExpresses FLP recombinase in DrosophilaDepositorInserthsFlp1
ExpressionInsectMutationAspartic Acid at Amino Acid 5PromoterHsp70Bb promoterAvailable SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-WHaloCaMP1a-EGFP
Plasmid#205314PurposeExpression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driverDepositorInsertWHaloCaMP1a-EGFP
ExpressionInsectAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only