4,932 results

1. UAS-Ceru-10unit

   Plasmid

   #158389

   Purpose

   10-unit intMEMOIR recording array for Drosophila melanogaster

   Depositor

   Michael Elowitz

   Article

   Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

   Insert

   10unit recording array
   

   Expression

   Insect

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ORCO-T2A-QF-DBD

   Plasmid

   #162525

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
   

   Expression

   Insect

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB1_hPMS2

   Plasmid

   #129425

   Purpose

   Expression human PMS2 protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human PMS2 (PMS2 Human)
   

   Expression

   Insect

   Promoter

   Polyhedrin promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac His6 TEV LIC cloning vector (4B)

   Plasmid

   #30115

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6-TEV

   Expression

   Insect

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. R702-X38-635: His6-Hs.NF1opt (1-2839) isoform 1

   Plasmid

   #159576

   Purpose

   Baculovirus protein expression of His6-Hs.NF1opt (1-2839) isoform 1

   Depositor

   Dominic Esposito

   Article

   Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

   Insert

   His6-Hs.NF1opt (1-2839) isoform 1 (NF1 Human)
   

   Tags

   His6

   Expression

   Insect

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-mCherry-PA-Rac1-T17N

   Plasmid

   #22029

   Purpose

   Expression of photoactivatable Rac1 (dominant-negative mutant).

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
   

   Tags

   6xHis and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac1-His10FLAG

   Plasmid

   #157736

   Purpose

   empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites

   Depositor

   Debra Hansen

   Article

   Olson et al Protein Expr Purif. 2021 May 7:105890. d

   Type

   Empty backbone

   Tags

   His10 + 3xFLAG, enterokinase-cleavable

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFasBac+GFP-CAMSAP2

   Plasmid

   #59037

   Purpose

   for baculovirus expression of CAMSAP2 in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP2 (CAMSAP2 Human)
   

   Tags

   His and eGFP

   Expression

   Insect

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. nos-Cas9.874Z1

    Plasmid

    #112685

    Purpose

    Express hSpCas9 under nanos promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    nanos

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1-OSF-TRIM5alpha(rhesus)

    Plasmid

    #79033

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(rhesus)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFB1_hMLH1

    Plasmid

    #129426

    Purpose

    Expression human MLH1 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MLH1 (MLH1 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT-Bip-GFP:V5:KDEL

    Plasmid

    #69917

    Purpose

    An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

    Depositor

    Kenneth Irvine

    Article

    Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

    Insert

    GFP
    

    Tags

    Drosophila BiP signal sequence, KDEL ER targeting…

    Expression

    Insect

    Promoter

    pMT

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIHEU-MCS

    Plasmid

    #58375

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han

    Article

    Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQUAST

    Plasmid

    #24349

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    5xQUAS-Pmin promoter
    

    Expression

    Insect

    Available Since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac1-Kozak-rhTRIM5alpha-PFOS

    Plasmid

    #79038

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    rhesus TRIM5alha-PFOS
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Arp2/3_pBIG2abcd

    Plasmid

    #173683

    Purpose

    Insect expression of the 7-subunit Human Arp2/3 complex

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Inserts

    Arp2
    Arp3
    ArpC2
    ArpC3-FLAG
    ArpC4
    ArpC5
    ArpC1
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

    Plasmid

    #55218

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

    Plasmid

    #30116

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFast-hHus1

    Plasmid

    #222469

    Purpose

    Expresses hHus1 in insect cells

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

    Insert

    hHus1 (HUS1 Human)
    

    Expression

    Insect

    Available Since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pIDK-HDAC1fl

    Plasmid

    #109158

    Purpose

    Encodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
    

    Expression

    Insect

    Promoter

    p10

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCFD3-ebony

    Plasmid

    #83380

    Purpose

    Drosophila gRNA expression plasmid targets ebony

    Depositor

    Richard Padgett

    Article

    Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

    Insert

    ebony (e Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 6X-STAT-synthetic luciferase

    Plasmid

    #37392

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    six STAT consensus sites
    

    Use

    Luciferase

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. IMPT-10316:GABR1

    Plasmid

    #157850

    Purpose

    HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

    Depositor

    Vadim Cherezov, Raymond Stevens

    Article

    Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

    Insert

    GABR1 (GABBR1 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p10xQUAS-CsChrimson

    Plasmid

    #163629

    Purpose

    10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

    Depositor

    Christopher Potter

    Article

    Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

    Insert

    CsChrimson.mVenus
    

    Tags

    mVenus

    Expression

    Insect

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 5XUAS IVS CsChrimson tdtomato_tr

    Plasmid

    #111545

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CsChrimson_tdtomato_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCoofy28

    Plasmid

    #44004

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His6-GST

    Expression

    Insect

    Promoter

    PPH

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pIEx4-Cre

    Plasmid

    #198280

    Purpose

    Expresses Cre in insect cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pIEx4-Cre
    

    Expression

    Insect

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pACU2_CD4-mIFP T2A HO1

    Plasmid

    #72441

    Purpose

    Expressing UAS-CD4-mIFP T2A HO1 in drosophila

    Depositor

    Xiaokun Shu

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    CD4-mIFP T2A HO1
    

    Expression

    Insect

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

31. Tubp-Gal80 in pCaSpeR4

    Plasmid

    #17748

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Tags

    SV40 polyA and Tubulin 1alpha promoter

    Expression

    Insect

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTriEx-mCerulean-PA-Rac1

    Plasmid

    #22030

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His and mCerulean

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pKC31

    Plasmid

    #226777

    Purpose

    Expresses Drosophila UNC-104(R562H) in insect cells

    Depositor

    Kyoko Chiba

    Article

    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

    Insert

    Drosophila unc-104 (R562H) (unc-104 Fly)
    

    Tags

    sfGFP and 2xStrepII

    Expression

    Insect

    Mutation

    changed Arginine 562 to Histidine (bris mutation)

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Copia Renilla Control

    Plasmid

    #38093

    Depositor

    Philip Beachy

    Article

    Lum et al Science. 2003 Mar 28;299(5615):2039-45.

    Insert

    renilla luciferase
    

    Use

    Luciferase

    Expression

    Insect

    Promoter

    copia

    Available Since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pCFD3-ovoD1

    Plasmid

    #111142

    Purpose

    Expresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogaster

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al G3 (Bethesda). 2018 Jul 31;8(8):2749-275

    Insert

    pCFD3-ovoD1 (ovo Fly)
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBIG1e-CDK9/Cyclin T1

    Plasmid

    #231732

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK9 (CDK9 Human)
    CCNT1 (CCNT1 Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFastBac His6 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-B)

    Plasmid

    #55219

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Tags

    His6

    Expression

    Insect

    Available Since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUASTattB-dALFANb(5mut)-mGreenLantern

    Plasmid

    #219661

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
    

    Expression

    Insect

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUASP dsRed Rab7 WT

    Plasmid

    #44824

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

40. ovoD1 D1B2NR (18 kb)

    Plasmid

    #37280

    Depositor

    Norbert Perrimon

    Article

    Chou et al Development. 1993 Dec;119(4):1359-69.

    Insert

    ovoD1 genomic DNA fragment
    

    Expression

    Insect

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only