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78,616 results

Showing: 3061 - 3090 of 78616 results

  1. hNTo1-qgRNA-pYJA5

    Plasmid
    #217781
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217781-plasmid-map-sequence-id-466019

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  5. InsRA-eGFP

    Plasmid
    #79795
    Purpose
    Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Article
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Tags
    eGFP extracellular
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79795-plasmid-map-sequence-id-149406

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  8. mCherry-Ezrin-N-14

    Plasmid
    #55043
    Purpose
    Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Ezrin (EZR Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55043-plasmid-map-sequence-id-271041

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  12. TFORF2768

    Plasmid
    #142159
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KDM5D (KDM5D Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142159-plasmid-map-sequence-id-397675

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  13. gRNA_Cloning Vector BbsI

    Plasmid
    #128433
    Purpose
    Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing
    Depositor
    Izuho Hatada
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    July 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    128433-plasmid-map-sequence-id-251645

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  17. pcDNA3-C-terminal GST

    Plasmid
    #128025
    Purpose
    Mammalian expression vector with C terminal GST tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    GST
    Expression
    Mammalian
    Available Since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128025-plasmid-map-sequence-id-254769

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  20. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid
    #139997
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139997-plasmid-map-sequence-id-352446

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  21. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Article
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  24. pT7-V5-SBP-C1-HsPTB1

    Plasmid
    #64925
    Purpose
    Expresses V5- and SBP-tagged PTB1 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    PTB1 (PTBP1 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64925-plasmid-map-sequence-id-109671

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  29. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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Showing: 3061 - 3090 of 78616 results