76,239 results
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pCMV-ABE8eWQ
Plasmid#161815PurposeExpresses ABE8eWQ in mammalian cellsDepositorInsertbpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
TPC1-G-GECO1.2
Plasmid#207143PurposeFusion of Ca2+ reporter and endosomal Ca2+-permeable channelDepositorInsertTPCN1 (TPCN1 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
2X PRE TK luc
Plasmid#11350DepositorInsertPRE
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Farnesyl-5
Plasmid#55295PurposeLocalization: Plasma Membrane, Excitation: 399, Emission: 456DepositorInsertFarnesyl (HRAS Human)
UseTagsmTagBFP2ExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
TPC2-G-GECO1.2
Plasmid#207141PurposeFusion of Ca2+ reporter and lysosomal Ca2+-permeable channelDepositorInsertTPC2N2 (TPCN2 Human)
UseTagsG-GECO1.2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/BLM
Plasmid#111766PurposeTo express human BLM protein in human cellsDepositorInsertBLM (BLM Human)
UseTagsFlag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX459v3
Plasmid#178799PurposeSpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shcGAS-Mmu
Plasmid#127698PurposeDoxycyclin inducible shRNA knockdown of mouse cGAS geneDepositorInsertshcGAS Mmu (Cgas Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A no ccDB (w48-1)
Plasmid#17398PurposeGateway entry 1A vector, no ccDB geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Anti-GST [N100/13R ]
Plasmid#188214PurposeMammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13DepositorInsertanti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_SABV_GPC
Plasmid#207305PurposeMammalian expression of Sabia virus GPC proteinDepositorInsertSABV_GPC
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP HA
Plasmid#59759Purposeencodes multiple copies of an epitope tag HADepositorInsertsmFP _HA
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_sfCherry3V(1-10)
Plasmid#117483PurposeLentiviral vector for constitutive expression of sfCherry3V(1-10)DepositorInsertsfCherry3V(1-10)
UseLentiviralTagsExpressionMammalianMutation6 substitutions (K45R, G52D, L83W, T106A, K182R, …PromoterSFFVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1b-V5
Plasmid#162518PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1b (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-GLT1a-V5
Plasmid#162517PurposeRat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loopDepositorInsertGLT1a (Slc1a2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-FLAG-CCPG1
Plasmid#128262PurposeExpresses CCPG1 in mammalian cellsDepositorInsertCCPG1 (CCPG1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCherry-3x-p67phox-GBD
Plasmid#214141PurposeRac activity sensorDepositorInsert3xp67phox-GBD (NCF2 Human)
UseTagsmCherryExpressionMammalianMutationOnly the GTPase binding domain (GBD) is includedPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pv9_CBX1_2xChromo-13XL-BASU
Plasmid#179412PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASUDepositorInsertCBX1.2xChromo-13XL-BASU
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Talin-TS
Plasmid#83376PurposeFRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted between V447 an…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Luciferase PGK-hygro
Plasmid#18782DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-mUbp43
Plasmid#12454DepositorInsertUBP43 (Usp18 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP703-N1
Plasmid#136558PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
ssGNb-mCherry
Plasmid#128788PurposeMammalian expressionDepositorInsertssGNb-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG:: mCherry-2a-hM4Dnrxn
Plasmid#52523PurposeCo-expresses hM4Dnrxn and a red fluorescent labelDepositorInsertmCherry-2a-hM4Dnrxn
UseTags2xHAExpressionMammalianMutationPromoterCAgAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VPR
Plasmid#183936PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VPR
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human ICAM-1
Plasmid#8632DepositorInsertICAM-1 (ICAM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
MS2-epegRNA-Dnmt1
Plasmid#211372PurposeDnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP productionDepositorInsertMS2-epegRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-C1 SNX9 RYK mutant R286A Y287A K288A
Plasmid#161993PurposeMammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tagDepositorInsertSNX9 (Snx9 Mouse)
UseTagsmCherryExpressionMammalianMutationR286A Y287A K288APromoterAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_FLUAV_HA
Plasmid#207273PurposeMammalian expression of Influenza A virus HA proteinDepositorInsertFLUAV_HA (HA Influenza A virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HLA-Flag-natT1R3
Plasmid#113950Purposemammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationresidues 21-852PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8f
Plasmid#176759PurposeAAV-mediated expression of membrane bound GCaMP8f under the control of GfaABC1D promoterDepositorHas ServiceAAV1 and AAV9Insertlck-jGCaMP8f
UseAAVTagsExpressionMammalianMutationPromoterGfaABC1DAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mCherry
Plasmid#27705DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric CherryExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-iLACCO1.2
Plasmid#218823PurposeMammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinityDepositorInsertiLACCO1.2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_EF1a_CasRx_Puro
Plasmid#134842PurposeLentiviral all in one expression of CasRx and guideRNADepositorInsertRfxCas13d
UseCRISPR and LentiviralTagsT2A PuromycinExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP2
Plasmid#22577DepositorInsertUSP2 (USP2 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
CBR1_pCSdest
Plasmid#53826DepositorInsertCBR1 (CBR1 Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
PB-TAC-ERN
Plasmid#80475PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-human APPL1
Plasmid#22198DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAPtag5-IgK-NCAN-Full length
Plasmid#219812PurposeExpresses His-tagged NCAN-Full length to produce recombinant proteinDepositorInsertIgK (Igk Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
TTVTFLPTPSLILQT_epitope
Plasmid#165021PurposeExpression of CMV UL44 (38-52) fused to CD74DepositorInsertCMV UL44 (38-52)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-R-GECO1
Plasmid#32444PurposeMammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
UseTagsExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
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hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
UseTagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hMLN
Plasmid#27079PurposeNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOGDepositorInsertC-MYC, LIN28, NANOG (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo
Plasmid#221899PurposeExpresses alpha tubulin fused to mStayGoldDepositorInsertmStayGold-aTubulin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
XBP-1 2: pFLAG.XBP1p.CMV2
Plasmid#21833DepositorInsertXBP-1 processed/spliced (Xbp1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-17-5p
Plasmid#103274PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-17-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-17-5p target (MIR17 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-17-3p
Plasmid#103273PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-17-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-17-3p target (MIR17 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
