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81,094 results

Showing: 3751 - 3780 of 81094 results

  1. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139988-plasmid-map-sequence-id-275338

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  3. EF1a_mCherry_P2A_Hygro

    Plasmid
    #135003
    Purpose
    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135003-plasmid-map-sequence-id-272070

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  7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118154-plasmid-map-sequence-id-239465

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  10. pAAV.hSyn.eGFP.WPRE.bGH

    Plasmid
    #105539
    Purpose
    AAV expression of EGFP from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105539-plasmid-map-sequence-id-457689

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  11. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

    Plasmid
    #105543
    Purpose
    AAV expression of EGFP from cTNT promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    cTNT-PI-EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    cTNT
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105543-plasmid-map-sequence-id-202437

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  14. N174-MCS (Puro)

    Plasmid
    #81068
    Purpose
    Lentiviral vector for constitutive gene expression.
    Depositor
    Adam Karpf
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    81068-plasmid-map-sequence-id-155844

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  15. pCDH-PGK

    Plasmid
    #72268
    Purpose
    Express gene of interest under PGK (phosphoglycerate kinase I) promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    PGK
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72268-plasmid-map-sequence-id-134573

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  22. mCherry-Rab7a-7

    Plasmid
    #55127
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab7a (RAB7A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55127-plasmid-map-sequence-id-311825

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  24. mKeima-Red-C1

    Plasmid
    #54546
    Purpose
    Localization: C1 Cloning Vector, Excitation: 440, Emission: 620
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKeima-Red
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54546-plasmid-map-sequence-id-256470

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  26. pcDNA3.1-MAP3K5 (WT)

    Plasmid
    #47104
    Purpose
    Expresses wild type human MAP3K5 in mammalian cells
    Depositor
    Yardena Samuels
    Insert
    MAP3K5 (MAP3K5 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    CMV
    Available Since
    Jan. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    47104-plasmid-map-sequence-id-214692

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Showing: 3751 - 3780 of 81094 results