75,952 results

1. SpCas9 TadCBEd-V106W

   Plasmid

   #193839

   Purpose

   Express TadCBEd-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDd V106W-SpCas9 D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF1169

   Plasmid

   #142745

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EBF1 (EBF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. IRF8-2A-neo

   Plasmid

   #190621

   Purpose

   Lentiviral expression plasmid of human IRF8, neomycin selection

   Depositor

   Junwei Shi

   Article

   Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

   Insert

   IRF8 (IRF8 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-D12L

   Plasmid

   #89161

   Purpose

   Express vaccinia virus D12L in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Vaccinia virus D12L
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promotor

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMGS7 (AAVS1sgRNA)

   Plasmid

   #126582

   Purpose

   A CRISPR/Cas9 construct to target the AAVS1 locus in humans

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AAVS1 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-Rab27B Q78L

   Plasmid

   #89448

   Purpose

   Expression of GFP-tagged human Rab27B Q78L in mammalian cells

   Depositor

   Wendy Westbroek

   Article

   Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

   Insert

   human Rab27B Q78L (RAB27B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q78L

   Promoter

   CMV

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. hAAVS1 TALEN Left

   Plasmid

   #52341

   Purpose

   TALEN vector for genomic targeting

   Depositor

   Su-Chun Zhang

   Article

   Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

   Insert

   AAVS1 TALEN Left
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-PM-ExRai-AktAR2

   Plasmid

   #184050

   Purpose

   Plasma membrane-targeted ExRai-AktAR2

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   PM-ExRai-AktAR2
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-lox-GFP shRNA p19-2

   Plasmid

   #14091

   Depositor

   Tyler Jacks

   Article

   Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

   Insert

   p19 ARF shRNA -2 (Cdkn2a Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.3_OCT4

    Plasmid

    #26816

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-OCT4-3'UTR (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. HA-GbL

    Plasmid

    #1865

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    GbL (MLST8 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag-TurboID-NES_pLX304

    Plasmid

    #202008

    Purpose

    expresses TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJRH-1363 LV EF1a-CD19 IRES-EGFP

    Plasmid

    #201919

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

    Plasmid

    #125256

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2(H134R)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a-forward

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-Centrin2-N-10

    Plasmid

    #55018

    Purpose

    Localization: Centrosomes, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Centrin2 (CETN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-TAC-ERP2

    Plasmid

    #80478

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. N-Cadherin-EGFP

    Plasmid

    #18870

    Depositor

    Valeri Vasioukhin

    Article

    Nechiporuk et al Dev Cell. 2007 Sep . 13(3):338-50.

    Insert

    N-Cadherin (Cdh2 Mouse)
    

    Use

    Tags

    EGFP, Kanamycin, and N-Cadherin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-opto-mCasp1

    Plasmid

    #208781

    Purpose

    Inducibly expresses mouse caspase1 fused to light activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mCaspase1 Cry2 (Casp1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry, Cry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmScarlet-H_Giantin_C1

    Plasmid

    #85049

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Giantin (GOLGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 3131-3259

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEYFP-NR1a

    Plasmid

    #17928

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    N-methyl-D-aspartate receptor NMDAR1 (Grin1 Rat)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid

    #207154

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA15 (GNA15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position E(244)N

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CYFIP1.mCherry

    Plasmid

    #122051

    Purpose

    Expresses human CYFIP1 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP1 (CYFIP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEGFP-Merlin

    Plasmid

    #84293

    Purpose

    Expression of wt Merlin

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Merlin (NF2) (NF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO-mito-mEm

    Plasmid

    #185596

    Purpose

    Lentiviral construct for expression of mEmerald targeted to mitochondria

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    mito-mEm
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-REPORT(PGK/CMV)-12xHRE

    Plasmid

    #172335

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. cavin-1-mEGFP

    Plasmid

    #27709

    Depositor

    Ari Helenius

    Article

    Hayer et al Traffic. 2010 Mar . 11(3):361-82.

    Insert

    cavin-1 (Cavin1 Mouse)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-Hygro(+)-mscGAS

    Plasmid

    #102607

    Purpose

    Mammalian expression of mouse cGAS

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (Cgas Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBabe-GFP-IKBalpha-wt

    Plasmid

    #15263

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-wtTnsAB

    Plasmid

    #234731

    Purpose

    Wild-type human-codon optimized PseTnsAB in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTnsAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-wtTnsC

    Plasmid

    #234732

    Purpose

    Wild-type human-codon optimized PseTnsC in mammalian cells

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    PseTnsC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-EF1a-DIO-JEDI-2P-WPRE

    Plasmid

    #179460

    Purpose

    Double floxed genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector under the control of the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV1

    Insert

    JEDI-2P
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. NPC1 His6 EGFP

    Plasmid

    #53521

    Depositor

    Matthew Scott

    Article

    Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

    Insert

    NPC1
    

    Use

    Tags

    EGFP and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pVC-FUS-WT

    Plasmid

    #175066

    Purpose

    expresses mVenus and mCherry tagged to FUS wildtype in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    FUS glycine rich protein (FUS Human)
    

    Use

    Tags

    mVenus + mCherry

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFUW-tetO-Myb

    Plasmid

    #139826

    Purpose

    doxycycline-inducible expression of mouse Myb in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Myb (Myb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P94H and Q619R- Please see depositor comment belo…

    Promoter

    TRE-mCMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Reduced Expression GFP beta actin

    Plasmid

    #31502

    Depositor

    Tim Mitchison, Rick Horwitz

    Article

    Watanabe et al Science. 2002 Feb 8. 295(5557):1083-6.

    Insert

    beta actin (ACTB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    91-544 of enhancer region of CMV promoter deleted

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TagBFP2-PIP4K2B

    Plasmid

    #202728

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    mTagBFP2:PIP4K2B (PIP4K2B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGART-mCherry

    Plasmid

    #167164

    Purpose

    Expresses human GART-mCherry fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Insert

    GART (GART Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MSCV-Tox-IRES-eGFP

    Plasmid

    #165427

    Purpose

    Expresses TOX in mammalian cells

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

    Insert

    TOX (Tox Mouse)
    

    Use

    Tags

    3xflag 5' of Tox

    Expression

    Mammalian

    Mutation

    Promoter

    5' LTR

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBA950

    Plasmid

    #122239

    Purpose

    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge