76,289 results
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Plasmid#173029PurposeFor expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.DepositorInsertpspCas13b
UseCRISPR and LentiviralTags3xFLAG, BFP, HIV NES, and T2AExpressionMammalianMutationPromoterEf1aAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
multi-shRNA vector
Plasmid#12391DepositorInsertmSOD2 shRNA (Sod2 Mouse)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-Sec23B
Plasmid#66598PurposeExpresses Sec23B as a fusion with mGFPDepositorInsertSec23B (SEC23B Human)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
FKBP:FRB-FBAR
Plasmid#223620PurposeTransient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cellsDepositorInsertFBP17 (FNBP1 Human)
UseTagsFKBP, TagBFP and mGFP, FRBExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-Flag-RECQL5
Plasmid#222620PurposeMammalian expression vector of flag-tagged RECQL5DepositorInsertRECQL5 (RECQL5 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-fDIO-Cre
Plasmid#121675PurposeExpresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)DepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9InsertNLS-CRE-HA
UseAAVTags3xHA and SV40-NLSExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-myrlysin/BORCS5
Plasmid#194332PurposeExpresses myrlysin in mammalian cellsDepositorInsertBORCS5 (BORCS5 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-APP-mGFP
Plasmid#196696PurposeExpresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsHA and mGFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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YEE-BE4max
Plasmid#138157PurposeC-to-T base editorDepositorInsertrAPOBEC1(YEE)-nSpCas9-UGI-UGI
UseTagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E + R132E; within Cas9…PromoterCMVAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
p4054 HA-E6AP isoform II
Plasmid#8658DepositorInsertE6-AP isoform II (UBE3A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
TFORF1216
Plasmid#144090PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNHLH1 (NHLH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla
Plasmid#60526PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-EGFP:LIMD1
Plasmid#108230PurposeLentiviral vector for Tet-inducible LIMD1 expressionDepositorInsertLIMD1 (LIMD1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTet/ON Minimal CMV promoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
LRP4-Flag/pCDNA3.1
Plasmid#197291PurposePlasmid expressing an antigen targeting LRP4 (extracellular)DepositorInsertLRP4 (Lrp4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-TEAD4-IDR
Plasmid#166451PurposeExpresses fusion of mCherry and TEAD4-IDRDepositorInsertmCherry, TEAD4-IDR(89-230) (TEAD4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas13d-BFP
Plasmid#215720PurposeCatalytically inactive dCasRx with 2A-BFPDepositorInsertdCasRx
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLII12Pglu-700uDelta6
Plasmid#17866DepositorInsertFLII12Pglu-700uDelta6
UseTagsCitrineExpressionMammalianMutation700uDelta6, ECFP insert in mglBPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
1227 pcDNA3 Myr HA Akt2
Plasmid#9016DepositorInsertAkt2 (AKT2 Human)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CRAF
Plasmid#51124Purposemammalian expression of wt CRAFDepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1
Plasmid#159585PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ATF6 (1-373)
Plasmid#27173PurposeFor mammalian expression of the cytoplasmic domain of human ATF6with an HA tag.DepositorInsertATF6 (ATF6 Human)
UseTagsHAExpressionMammalianMutationContains only aa 1-373 (please see depositor comm…PromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pC016 - LwCas13a guide expression backbone with U6 promoter
Plasmid#91906PurposeBackbone for cloning LwCas13a guides under U6 promoterDepositorHas ServiceCloning Grade DNAInsertLwCas13a Guide site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron
Plasmid#235087PurposeGFP splicing reporter with FRG1 intronDepositorInsertBFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)
UseAAVTagsExpressionMammalianMutationPromotercustom bidirectionalAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc hygro
Plasmid#67502PurposeConstitutive expression of firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-VPS13C-Cter
Plasmid#232866PurposeExpresses the C-terminal fragments of VPS13C in mammalian cellsDepositorInsertthe C-terminal fragments of VPS13C (VPS13C Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DENV1_PrME
Plasmid#207263PurposeMammalian expression of Dengue virus 1 PrME proteinDepositorInsertDENV1_PrME (POLY Dengue virus 1)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-HALOnls
Plasmid#121937PurposeCRISPR-Sirius plasmidDepositorInsertMCP-HALOnls
UseLentiviralTagsHaloTagExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOf
Plasmid#189932PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-StAR4B (Lenti)
Plasmid#222691PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPGK without BsmBI cutsitePromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mNeonGreen
Plasmid#98876PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsert4xmts
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CHMP2A_GFP_N_term
Plasmid#31805DepositorInsertCHMP2A (CHMP2A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP20
Plasmid#22573DepositorInsertUSP20 (USP20 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab4B
Plasmid#61801PurposeHuman Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expressionDepositorInsertRab4B (RAB4B Human)
UseTagsGFPExpressionMammalianMutationNonePromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
tetO-SMAD6
Plasmid#170700Purposedoxycycline-inducible overexpression of SMAD6DepositorInsertSMAD6 (SMAD6 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-EF1α-mKate2-PDE4D3-Cat
Plasmid#169128PurposeAAV plasmid containing a mKate2 and a truncated PDE4D3DepositorUseAAV and Cre/LoxTagsmKate2ExpressionMammalianMutationtruncated, Catalatic domain onlyPromoterEF1aAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
BASU RaPID plasmid
Plasmid#107250PurposeExpresses the BASU RaPID labeling proteinDepositorInsertBASU
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nMagHigh1-EGFP-CAAX
Plasmid#67300PurposeBlue light dependent photoswitching systemDepositorInsertnMagHigh1-EGFP-CAAX
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-GBP1-10gly-lexADBD
Plasmid#50795PurposeExpression of a GFP-dependent transcription factor component in mammalian cellsDepositorInsertGBP1-lexA DNA binding domain fusion protein
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-DRP1 K38A
Plasmid#232350PurposeExpresses K38A variant of human DRP1 with mCherry tagDepositorInsertDRP1 (DNM1L Human)
UseTagsmCherryExpressionMammalianMutationK38APromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
dCas12Max-CBE
Plasmid#195337Purposevector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorInserthuman codon-optimized dCas12Max-CBE
UseTagsExpressionMammalianMutationN243R, E336R, D1049APromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-RNF2_+5GtoT
Plasmid#135957PurposeS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editingDepositorInsertRNF2_5GtoT pegRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF Synaptophysin-mRuby
Plasmid#188980PurposeSynaptic buton markerDepositorInsertSynaptophysin (Syp Synthetic, Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
HRE-dUnaG
Plasmid#201711PurposeHypoxia responsive promoter driving destablized UnaG fluorescent proteinDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
phU1-PspCas13b-non-targeting (pEZ36)
Plasmid#229053PurposeCloning backbone for PspCas13b crRNA (Human U1 promoter)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) SDC3
Plasmid#107501PurposeExpresses SDC3, puromycin resistantDepositorInsertSDC3 (codon optimized) (SDC3 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
evoBE4-SpRY
Plasmid#226854PurposeExpresses evoBE4max-SpRY base editor in mammalian cells with eGFP markerDepositorInsertevoBE4max-SpRY
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only