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pLenti CMVTRE3G eGFP Neo (w821-1)

Plasmid

#27569

Depositor

Eric Campeau

Insert

Enhance Green Fluorescent Protein

Use

Lentiviral

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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CDK2 gRNA (BRDN0001148950)

Plasmid

#77193

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK2 (CDK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-LACTB-dAPEX2

Plasmid

#117179

Purpose

Plasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KMT2A_MLLT1

Plasmid

#205842

Purpose

Express mEGFP-tagged fusion protein, KMT2A_MLLT1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KMT2A_MLLT1 (KMT2A, MLLT1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EKAR4

Plasmid

#174437

Purpose

Improved cyan/yellow FRET-based ERK kinase activity reporter.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

EKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KMT2A_MLLT10

Plasmid

#205843

Purpose

Express mEGFP-tagged fusion protein, KMT2A_MLLT10 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KMT2A_MLLT10 (KMT2A, MLLT10 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-1

Plasmid

#50742

Purpose

Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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plex_307-ccdB-g418

Plasmid

#201141

Purpose

pLEX_307 plasmid with g418 marker

Depositor

Alejandro Chavez

Type

Empty backbone

Expression

Mammalian

Promoter

EF-1α

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowG

Plasmid

#104620

Purpose

Expresses Shadow G in mammalian cell

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2015 Oct 15;5:15334. doi: 10.10

Insert

ShadowG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCVpuro-tdTomato

Plasmid

#97079

Purpose

Retroviral plasmid containing tdTomato for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

tdTomato

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF2

Plasmid

#200382

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF0

Plasmid

#200380

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor ORF1

Plasmid

#200381

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-NFKB1

Plasmid

#139822

Purpose

doxycycline-inducible expression of Human NFKB1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

NFKB1 (NFKB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Bcl11a

Plasmid

#139809

Purpose

doxycycline-inducible expression of human Bcl11a in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

BCL11a (BCL11A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCS

Plasmid

#12158

Depositor

Inder Verma

Article

Miyoshi et al J Virol. 1998 Oct . 72(10):8150-7.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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EGFP-AR

Plasmid

#111215

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

AR (Ar Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

Plasmid

#139091

Purpose

Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

dSpCas9-NG-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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PL-5LTR-RGR(DMD#1)-AmCyan-A

Plasmid

#138482

Purpose

Expresses sgRNA targeting human DMD (Dystrophin) for packaging into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Ribozyme-flanked gRNA and AmCyan

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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