Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

Your Selections

Mammalian Expression

76,418 results

Showing: 11251 - 11300 of 76418 results

  10. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

    Enlarge

  12. pAK_EF1a_CasRx_Hygro

    Plasmid
    #134840
    Purpose
    Lentiviral expression of CasRx T2A Hygromycin
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Tags
    T2A Hygromycin
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134840-plasmid-map-sequence-id-266452

    Enlarge

  13. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Insert
    centrin-1 (CETN1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72641-plasmid-map-sequence-id-132790

    Enlarge

  14. TFORF2027

    Plasmid
    #143994
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NPAS2 (NPAS2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    143994-plasmid-map-sequence-id-430156

    Enlarge

  17. Lenti-EF1alpha-ZsGreen1_Blast

    Plasmid
    #192660
    Purpose
    3rd generation lenti vector encoding ZsGreen1 with 2A Blast resistance marker
    Depositor
    Jeffrey Miner
    Insert
    ZsGreen1
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192660-plasmid-map-sequence-id-379934

    Enlarge

  23. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

    Plasmid
    #55613
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (WT) (HERC2 Human)
    Use
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    Silent mutations have been incorporated into the …
    Promoter
    Available since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55613-plasmid-map-sequence-id-86499

    Enlarge

  32. Kaede-C2

    Plasmid
    #54725
    Purpose
    Localization: C2 Cloning Vector, Excitation: 508 / 572, Emission: 518 / 580
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    Kaede
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    54725-plasmid-map-sequence-id-482901

    Enlarge

  38. TFORF0725

    Plasmid
    #143071
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFKB2 (NFKB2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143071-plasmid-map-sequence-id-399051

    Enlarge

  39. pHAGE mCherry-Rab11A

    Plasmid
    #228035
    Purpose
    Mammalian lentiviral expression of mCherry-labeled Rab11.
    Depositor
    Wade Harper
    Insert
    Rab11A (RAB11A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228035-plasmid-map-sequence-id-450376

    Enlarge

  41. PERK 6: PERK.9E10.pBABE

    Plasmid
    #21819
    Depositor
    David Ron
    Insert
    PERK (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    Myc (9E10)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 10, 2010
    Availability
    Academic Institutions and Nonprofits only
    21819-plasmid-map-sequence-id-10015

    Enlarge

  44. pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc

    Plasmid
    #223559
    Purpose
    Alias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotor
    Depositor
    Mikel Garcia-Marcos
    Article
    Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457
    Insert
    YFP(Venus) - KRas4b - IRES* - human GINIP - linker(GGGS) - Nluc
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 28, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    223559-plasmid-map-sequence-id-443639

    Enlarge

  46. hTLR2 flag

    Plasmid
    #13082
    Depositor
    Ruslan Medzhitov
    Insert
    TLR2 (TLR2 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-16 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13082-plasmid-map-sequence-id-142444

    Enlarge

  48. pEGFP rbFOX2

    Plasmid
    #63086
    Purpose
    Mammalian expression of EGFP tagged FOX2
    Depositor
    Nicolas Charlet-Berguerand
    Insert
    RBFOX2 (RBFOX2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63086-plasmid-map-sequence-id-104485

    Enlarge

Showing: 11251 - 11300 of 76418 results