80,589 results
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U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA
Plasmid#171383PurposeExpression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized minidCas13X.1-REPAIRv2
TagsHAExpressionMammalianPromoterCMV, U6Available SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Blast
Plasmid#227270PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-mCherry-FRB
Plasmid#172443PurposeExpression of plasma membrane protein Stargazin tagged with mCherry-FRBDepositorAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDG462
Plasmid#100903PurposeSpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianMutationD10A mutant converts to NickasePromoterCBh and U6Available SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF1338
Plasmid#142787PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pomc-pGL3
Plasmid#17553PurposeLuciferase expression under control of Pomc promoter (ā646 to +65).DepositorAvailable SinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only -
OR52-consensus
Plasmid#208080PurposeConsensus OR52 engineered from human OR52 family membersDepositorInsertOR52-consensus
TagsRhodopsin tagExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GH
Plasmid#60514PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HaloGFP-Na2.4
Plasmid#228471PurposeMammalian expression of green fluorescent sodium ion indicator HaloGFP-Na2.4DepositorInsertHaloGFP-Na2.4
ExpressionMammalianAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-TEAD1
Plasmid#33109DepositorAvailable SinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHS18 (mANGPTL3-Strep)
Plasmid#109111PurposeExpresses StrepII-tagged mouse ANGPTL3 in mammalian cellsDepositorAvailable SinceJune 5, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Flag-hGSDMD
Plasmid#218938PurposeExpression of N-terminal FLAG tagged human gasdermin DDepositorAvailable SinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
TagsneoTET cassette with a tetrahymena self-splicing ā¦ExpressionMammalianPromoter7SL upstream pollII enhancerAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mito-mEosEM
Plasmid#132706Purposeapplication in Correlative light-electron microscopyDepositorInsertmitochondria targeting sequence
TagsmEosEMExpressionMammalianPromoterCMVAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAI-Env
Plasmid#133996PurposeEnv gene from HIV-1 LAI in pcDNA3.1 expression vectorDepositorInsertEnvelope Glycoprotein
ExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
EMTB-3XGFP
Plasmid#26741DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags3x EGFP and MycExpressionMammalianMutationOnly contains the microtubule binding-domain of eā¦Available SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
PSMC2_GFP_Cterm
Plasmid#221516PurposeFluorescently tagged Psmc2-GFPDepositorAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGreenPuro-shRNA-Stx3S-C4
Plasmid#99742Purpose3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.DepositorInsertshRNA against Stx3S
UseLentiviral and RNAiExpressionMammalianPromoterH1Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
1098 pcDNA GFP FKHR
Plasmid#9022DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-PH-BTKx2
Plasmid#183654PurposePtdIns(3,4,5)P3 biosensorDepositorInsertBTK (BTK Frog, Human)
TagsX. laevis map2k1.L(32-44)-mCherryExpressionMammalianMutationAmino acids 2-174 and 2-170PromoterCMVAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
myc-Rictor
Plasmid#1860PurposePlease see Addgene item #11367 for an updated version of this plasmid.DepositorAvailable SinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
WWP-Luc (p21/WAF1 promoter)
Plasmid#16451DepositorAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF1275
Plasmid#143781PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APPP1-mGFP
Plasmid#196705PurposeAs mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activityDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsmCherry and mEGFPExpressionMammalianMutationLys to Val substitution at position 612Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT3(E338Q)
Plasmid#164696PurposeExpression of PRMT3(E338Q) with an N-terminal FLAG tagDepositorAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Full human ELL2
Plasmid#127253PurposeExpresses full-length human ELL2 in mammalian cellsDepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pScalps_Puro
Plasmid#99636PurposeEmpty backbone, 3rd generation lentiviral vectorDepositorTypeEmpty backboneUseLentiviralTagsnoneExpressionMammalianPromoterSFFVpAvailable SinceAug. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mNeonGreen-NLS-blast
Plasmid#136258PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmNeonGreen-NLSExpressionMammalianAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
HBx
Plasmid#65463Purposeexpresses HBx in mammalian cellsDepositorInsertHBV X protein
TagsFlagExpressionMammalianAvailable SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-THP-Synaptophysin-pHluorin
Plasmid#242903PurposeFluorescent reporter for imaging of synaptic vesicle recycling inĀ Dopaminergic NeuronsDepositorInsertsynaptophysin plus pHluorin
UseAAVTagsmouse synaptophysinExpressionMammalianPromoterrat Tyrosine hydroxylaseAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only -
ARL13B-(ĪGTPase)- GFP
Plasmid#246843PurposeExpress Arl13B (resistance to sgRNA) with deletion of GTPase, tagged with GFPDepositorInsertArl13B (ARL13B Human)
UseLentiviralTagsGFPExpressionMammalianMutationdeleted GTPase domainAvailable SinceDec. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCGT001_nuclear-mOrange2
Plasmid#248083PurposeConstitutive expression of NLS-tagged mOrange2 from EF1-alpha promoter (for lentiviral packaging)DepositorInsertmOrange2
UseLentiviralTagsSV40 NLSExpressionMammalianPromoterEF1-alphaAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-Src Y527E
Plasmid#246428PurposeExpresses Src mutant Y527E (aka Y530E) in Mammalian cellsDepositorAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Flag-Src Y527A
Plasmid#246429PurposeExpresses Src mutant Y527A (aka Y530A) in Mammalian cellsDepositorAvailable SinceOct. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-HSPB-Cre
Plasmid#235217PurposeCre expression under the control of HSPB promoter; Tet-On 3G-inducible PiggyBac system for expression in human pluripotent stem cells.DepositorInsertHSPB-Cre
UseCre/LoxExpressionMammalianAvailable SinceAug. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-HSP90AB1 Fusion Vector
Plasmid#237196PurposeExpress NanoLuc(R)-HSP90AB1 Fusion Protein in Mammalian Cells under a CMV promoterDepositorAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
PD-L1-NanoLuc Fusion Vector
Plasmid#237129PurposeExpress PD-L1-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertPD-L1 (CD274 Human)
TagsNanoLuc (R)ExpressionMammalianMutationNonePromoterCMVAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
iC30 PB-Zim3-dCas9-P2A-BFP_hygro
Plasmid#236743PurposeConstitutive expression of Zim3-dCas9InsertsCAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
EF1a-hygro-WPRE
ExpressionMammalianPromoterCAG and EF1aAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BubR1
Plasmid#200814Purposeexpresses BubR1 in mammalian cellsDepositorInsertBUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
TagsFLAGExpressionMammalianPromoterCAGAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only
