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80,556 results

Showing: 1801 - 1840 of 80556 results

  2. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid
    #72299
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer
    Available Since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72299-plasmid-map-sequence-id-131901

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  8. pLV-SFFV-MCS-WPRE-UbC-mCherry

    Plasmid
    #211799
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    multiple cloning site
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available Since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211799-plasmid-map-sequence-id-418073

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  10. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  18. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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  22. pAF112

    Plasmid
    #135920
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Luciferase; Transposon
    Expression
    Mammalian
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135920-plasmid-map-sequence-id-270078

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  24. pEGFP-C1-NuMA

    Plasmid
    #81029
    Purpose
    Expresses human NuMA with N-terminal EGFP tag in mammalian cells
    Depositor
    Michael Mancini
    Insert
    Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    81029-plasmid-map-sequence-id-300392

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  26. PGKp-GFP-YAP (5SA)

    Plasmid
    #174174
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    PGK
    Available Since
    Aug. 19, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174174-plasmid-map-sequence-id-340277

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  27. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Available Since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  29. FPR2-Tango

    Plasmid
    #66284
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    FPR2 (FPR2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    66284-plasmid-map-sequence-id-263479

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  39. piggyBAC-BRD4-Flag

    Plasmid
    #214432
    Purpose
    A piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.
    Depositor
    Shaun Cowley
    Insert
    Brd4 bromodomain containing 4 (Brd4 Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    214432-plasmid-map-sequence-id-437159

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Showing: 1801 - 1840 of 80556 results