76,969 results

1. FU-tetO-Gateway

   Plasmid

   #43914

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector, doxycycli…

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. B-gTEMP/pcDNA3

   Plasmid

   #188447

   Purpose

   B-gTEMP for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHCMV_DENV2_PrME

   Plasmid

   #207264

   Purpose

   Mammalian expression of Dengue virus 2 PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   DENV2_PrME (POLY Dengue virus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG G-CEPIA1er

   Plasmid

   #105012

   Purpose

   ER calcium sensor

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   G-CEPIA1er
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-CAG-jGCaMP8s-WPRE

   Plasmid

   #179256

   Purpose

   AAV-mediated expression of GCaMP8s under the CAG promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-polyQ23-NLS

   Plasmid

   #231891

   Purpose

   Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q23-NLS (HTT Human)
   

   Use

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CD8a-EGFP

   Plasmid

   #86051

   Purpose

   A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-C-terminal GST

   Plasmid

   #128025

   Purpose

   Mammalian expression vector with C terminal GST tag

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX304 CMV FKBP-V5-sTurboID (N)

    Plasmid

    #153002

    Purpose

    Expresses low-affinity split-TurboID N-terminal fragment in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-R-diLACCO1

    Plasmid

    #208028

    Purpose

    Control biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-diLACCO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ER-RpHLuorin2

    Plasmid

    #171717

    Purpose

    Luminal ER expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 targeted to ER
    

    Use

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDG462

    Plasmid

    #100903

    Purpose

    SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Villinpromoter-blue-FlpOERT2

    Plasmid

    #67278

    Purpose

    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Insert

    Villin-mTQ2-P2A-FlpO-ERT2
    

    Use

    Tags

    mTQ2, linked to FlpO through an P2A ribosomal ski…

    Expression

    Mammalian

    Mutation

    Promoter

    Villin

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE2-EF1a-GFP1-10-IRES-Puro

    Plasmid

    #211467

    Purpose

    Lentivirus backbone expressing GFP1-10

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FL-fibulin-3-V5-lentiviral-GFP

    Plasmid

    #29703

    Depositor

    Mariano Viapiano

    Article

    Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

    Insert

    EFEMP1 (EFEMP1 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pSL3718 (pQCascade_Pse)

    Plasmid

    #200899

    Purpose

    Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCas7, PseCas8, PseCas6, PseTniQ
    

    Use

    Tags

    NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFUGW-FerH-ffLuc2-eGFP

    Plasmid

    #71393

    Purpose

    Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

    Depositor

    Glenn Merlino

    Article

    Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

    Insert

    FerH-ffLuc2-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FerH

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PIEZO1-HaloTag

    Plasmid

    #207834

    Purpose

    PIEZO1 fusion for biochemistry and live imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    PIEZO1 (PIEZO1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge