81,267 results

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pBABE hBRM

Plasmid

#1961

Depositor

Robert Kingston

Article

Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Insert

BRM (SMARCA2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

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Cdk4-HA

Plasmid

#1876

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-ACE2

Plasmid

#141185

Purpose

Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-eGFP

Plasmid

#99375

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-eGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAVU6+27-F30-2xdBroccoli

Plasmid

#66842

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Mammalian

Promoter

U6

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-Brainbow3.0

Plasmid

#45176

Depositor

Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pCAG-Brainbow3.0

Expression

Mammalian

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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wtTDP43tdTOMATOHA

Plasmid

#28205

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

HA tag and tdtomato

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-DsRed Express

Plasmid

#22724

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

a variant of Discosoma sp. red fluorescent protein

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR4-GFP-C1 (w392-1)

Plasmid

#17396

Purpose

Gateway entry vector for N-term eGFP fusion, frame C1

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Gateway Cloning

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas G12V

Plasmid

#18666

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

G12V mutation in HRas

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pST1374

Plasmid

#13426

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-Cas9

Plasmid

#181752

Purpose

MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLV-Gag-3xNES-Cas9 (Spy)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2WT

Plasmid

#101221

Purpose

Blue-light dependent optogenetic protein Cry2

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2PHR

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLSC-5

Plasmid

#62889

Purpose

Lenti vector for expression of inducible split Cas9

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Inserts

SpCas9(574-1368)
SpCas9(2-573)

Use

CRISPR and Lentiviral

Tags

FFBP12, FRB, NES PTK2, NLS SV40, and P2A

Expression

Mammalian

Mutation

*see comment below

Promoter

EFS

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-P4M-SidMx2

Plasmid

#51472

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Inserts

P4M-SidM(546-647)
P4M-SidM(546-647)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEF6.mCherry-TSG101

Plasmid

#38318

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

TSG101 (TSG101 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pMA2640

Plasmid

#25434

Purpose

Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

reverse tetracycline-regulated transactivator Advanced

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-WT

Plasmid

#25361

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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Myc-TEAD4

Plasmid

#24638

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD-4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

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PL-SIN-Oct4-EGFP

Plasmid

#21319

Purpose

Lentiviral vector, expresses EGFP under Oct3/4 promoter

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein

Use

Lentiviral

Expression

Mammalian

Promoter

Oct-4 promoter region

Available Since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pCGN-SRF

Plasmid

#11977

Purpose

For mammalian expression of human SRF with an HA tag

Depositor

Ron Prywes

Article

Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

Insert

SRF (SRF Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-c-Myc-T58A

Plasmid

#13372

Purpose

Retroviral expression of mouse c-Myc T58A mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (T58A mutant) (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Tyrosine 58 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
FAS promoter luciferase

Plasmid

#8890

Depositor

Bruce Spiegelman

Article

Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

Insert

Fatty Acid Synthetase Promoter (Fasn unknown - rat or mouse, Rat)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
RG6

Plasmid

#80167

Purpose

fluorescent protein splicing reporter

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Expression

Mammalian

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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81,267 results