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Mammalian Expression

76,413 results

Showing: 2081 - 2120 of 76413 results

  1. pcDNA3-endo-GsCT

    Plasmid
    #205516
    Purpose
    Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-GsCT (GNAS Human, Synthetic)
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205516-plasmid-map-sequence-id-409476

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  4. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

    Plasmid
    #154773
    Purpose
    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y125A in G1PylS, hybrid PylT with A41AA and C55A …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154773-plasmid-map-sequence-id-310428

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  11. pLV_CAG_Gag-MCP

    Plasmid
    #212637
    Purpose
    Lentivirus vector to express an MLV Gag-MCP fusion protein
    Depositor
    Paul Blainey
    Article
    Najia et al bioRxiv 2024.10.01.616098
    Insert
    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    Use
    Lentiviral
    Tags
    MS2 Capsid Protein (N55K)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    212637-plasmid-map-sequence-id-422309

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  12. APJ-Tango

    Plasmid
    #66224
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    APJ (APLNR Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pCB6-EnvA

    Plasmid
    #74420
    Purpose
    Mammalian expression of the subgroup A avian leukosis-sarcoma envelope glycoprotein (EnvA)
    Depositor
    Judith White
    Article
    Gilbert et al J Virol. 1994 Sep;68(9):5623-8.
    Insert
    Env from ASLV-A
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  25. pc DNA FRT TO- APP695

    Plasmid
    #114193
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild type splice variant 695: 695AA (2085bp, lack…
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114193-plasmid-map-sequence-id-224265

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  26. pcDNA3.1-Flag-gp130dimer

    Plasmid
    #174394
    Purpose
    Cytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).
    Depositor
    Dennis Ko
    Article
    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-
    Insert
    IL6ST cytosolic domain with leucine zipper & C-C linker
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174394-plasmid-map-sequence-id-341883

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  28. NG-ABE9

    Plasmid
    #240625
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Article
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Insert
    TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…
    Promoter
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240625-plasmid-map-sequence-id-479197

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  31. pLIX403-EYFP-ccdB-Zeo

    Plasmid
    #158565
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158565-plasmid-map-sequence-id-309880

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  33. TFORF2848

    Plasmid
    #144249
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF21 (TCF21 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144249-plasmid-map-sequence-id-391481

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  34. pJCX4

    Plasmid
    #170756
    Purpose
    Express in mammalian cells a target protein C-terminally fused to the Mxe GryA intein - chitin binding domain (CBD) tag.
    Depositor
    Roberto Dominguez
    Article
    Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi
    Type
    Empty backbone
    Use
    Tags
    Mxe GyrA intein - chitin binding domain (CBD)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170756-plasmid-map-sequence-id-334719

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  37. pLenti4/V5-DEST-P85

    Plasmid
    #40219
    Depositor
    Lynda Chin
    Insert
    P85 alpha (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 2081 - 2120 of 76413 results