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pAAV-EF1α-GRAB_PGE2-1.0

Plasmid

#251355

Purpose

Express the PGE2 sensor GRAB_PGE2-1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al bioRxiv [Preprint]. 2025 Jun 3:2025.06.0

Insert

PGE2 sensor GRAB_PGE2-1.0

Use

AAV

Expression

Mammalian

Promoter

EF1α

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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hOT-GFP

Plasmid

#67848

Purpose

mammalian expression of human oxytocin receptor with GFP tag

Depositor

Christian Gruber

Article

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

oxytocin receptor (OXTR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
cytoplasmic NAD+ biosensor

Plasmid

#186787

Purpose

NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

cytoplasmic NAD+ biosensor

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-BBz

Plasmid

#135992

Purpose

Modular 41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-41BB-3z-P2A-EGFP (CD247, CD28, TNFRSF9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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nuclear NAD+ biosensor

Plasmid

#186789

Purpose

NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

nuclear NAD+ biosensor

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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T7opt in pCAGGS

Plasmid

#65974

Purpose

Human codon-optimized T7 polymerase

Depositor

Benhur Lee

Article

Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

Insert

T7-opt

Expression

Mammalian

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-(-)-Pur

Plasmid

#200458

Purpose

Eukaryotic expression vector

Depositor

Bertrand Collet

Type

Empty backbone

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Article

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCW-Cas9

Plasmid

#50661

Purpose

Doxycycline-inducible lentiviral expression of SpCas9

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

humanized S. pyogenes Cas9

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Tet ON

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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SP-dCas9-VPR

Plasmid

#63798

Purpose

SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

SP-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pXR001: EF1a-CasRx-2A-EGFP

Plasmid

#109049

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

CasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro DEST (w529-2)

Plasmid

#19068

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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mito cpVenus control

Plasmid

#186792

Purpose

Identical to mito NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

Depositor

Michael Cohen

Article

Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

Insert

mito cpVenus control

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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MSCVneo-HA-ER-Hoxb8

Plasmid

#222291

Purpose

The most commonly used plasmid for the generation of ER-Hoxb8 conditionally immortalized myeloid cell lines.

Depositor

Mark Kamps, David Sykes

Article

Wang et al Nat Methods. 2006 Apr;3(4):287-93.

Insert

ER-Hoxb8 (ESR1, Hoxb8 Human, Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

The estrogen receptor hormone binding domain cont…

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MAT2A

Plasmid

#100521

Purpose

Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

MAT2A (MAT2A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTight TRE

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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v5 Cas9-eVLP

Plasmid

#228468

Purpose

Expresses MMLVgag(C507V)–Cas9 for producing v5 Cas9-eVLPs

Depositor

David Liu

Article

Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

Insert

MMLVgag(C507V)–Cas9

Tags

FLAG

Expression

Mammalian

Mutation

MMLVgag(C507V)

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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3336 pcDNA3 Bcl-2

Plasmid

#8768

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pCAG-IRES2-p75NTR-GFP

Plasmid

#182947

Purpose

To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal

Depositor

Pantelis Tsoulfas

Insert

p75NTR-mGFP

Tags

HA tag and mGFP

Expression

Mammalian

Mutation

The leader sequence is from preprotrypin

Promoter

CAG

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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