81,268 results

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pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pN1 Lck-GCaMP5G

Plasmid

#34924

Purpose

Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

Depositor

Baljit Khakh

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

Lck-GCaMP5G

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro empty (w214-1)

Plasmid

#17484

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-cMYC

Plasmid

#20324

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

cMYC (Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-CUL5

Plasmid

#19895

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

cullin 5 (CUL5 Human)

Tags

myc3

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDESTmycDICER

Plasmid

#19873

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

dicer 1, ribonuclease type III (DICER1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pBabe-c-myc-zeo

Plasmid

#17758

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

c-myc (MYC Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.Luc.IRES.EGFP.SV40

Plasmid

#105533

Purpose

AAV expression of Luciferase and EGFP from CMV promoter

Depositor

James M. Wilson

Insert

Luciferase

Use

AAV and Luciferase

Tags

IRES-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-STING

Plasmid

#102598

Purpose

Express STING for IFN-beta luciferase reporter assay.

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ADRP

Plasmid

#87161

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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murine TCR OTII-2A.pMIG II

Plasmid

#52112

Purpose

retroviral expression of both TCRalpha and TCRbeta using a single vector

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Inserts

T cell receptor alpha chain (Tcra Mouse)
T cell receptor beta chain (Tcrb Mouse)

Use

Retroviral

Tags

P2A (from porcine teschovirus-1)

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight Puro DEST (w768-1)

Plasmid

#26430

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pMXs-Hu-L-Myc

Plasmid

#26022

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

L-Myc (MYCL Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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VCP(DK0)-EGFP

Plasmid

#23974

Depositor

Nico Dantuma

Article

Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Insert

Valosin containing protein (VCP Human)

Tags

6xHis, EGFP, and HA

Expression

Mammalian

Mutation

E305QE578Q

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Ataxin3Q84

Plasmid

#22123

Depositor

Henry Paulson

Article

Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

Insert

Ataxin 3 (ATXN3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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EGFP-GalT

Plasmid

#11929

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pSV Sport SREBP-1c

Plasmid

#8883

Depositor

Bruce Spiegelman

Article

Tontonoz et al Mol Cell Biol. 1993 Aug . 13(8):4753-9.

Insert

SREBP-1c (Srebf1 Mouse)

Expression

Mammalian

Mutation

Active version of SREBP-1c.

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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phage UbiC G3BP1-GFP-GFP

Plasmid

#119950

Purpose

Lentiviral vector expressing G3BP1-GFP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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px330-mcherry

Plasmid

#98750

Purpose

Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

Depositor

Jinsong Li

Article

Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-ccdB-3xFLAG-V5

Plasmid

#87071

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1- dCas9-2xNLS-EGFP

Plasmid

#74710

Purpose

Expresses dCas9-2xNLS-EGFP in mammalian cells

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

dCas9-2xNLS-EGFP

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCS6-YFP-SMASh

Plasmid

#68853

Purpose

Expresses SMASh tag fused to YFP at C terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

YFP-SMASh

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sh1368 knockdown c-myc

Plasmid

#29435

Depositor

Bob Eisenman

Article

Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

Insert

c-Myc shRNA (MYC Human)

Use

Lentiviral and RNAi

Tags

GFP and WPRE

Expression

Mammalian

Available Since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlag pCMV5-TOPO TAZ WT

Plasmid

#24809

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAc-Neo-OVA

Plasmid

#22533

Depositor

Michael Bevan

Article

Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Available Since

Dec. 28, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

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81,268 results