78,292 results

1. AAVS1_Puro_hPGK1_EGFP_Donor

   Plasmid

   #178088

   Purpose

   HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human PGK1

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIP-SFFV-mtagBFP-2A STING V155M

   Plasmid

   #102591

   Purpose

   Lentivector to express STING V155M and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   V155M

   Promoter

   SFFV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. EB3-mScarlet-I

   Plasmid

   #98826

   Purpose

   In vivo visualization of microtuble plus ends (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   EB3
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Syn-DIO-PinkyCaMP

   Plasmid

   #232857

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBbi G418R mbIL21

   Plasmid

   #194329

   Purpose

   Consitiutive mammalian expression of membrane bound human IL-21

   Depositor

   Alexander McLellan

   Article

   Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

   Insert

   mbIL-21 (IL21 Human)
   

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA_AAVS1-T2

   Plasmid

   #41818

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCKO2

   Plasmid

   #125518

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pALB-GFP

   Plasmid

   #55759

   Purpose

   Fluorescent marker for hepatocytic lineage

   Depositor

   Snorri Thorgeirsson

   Article

   Heo et al Hepatology. 2006 Dec;44(6):1478-86.

   Insert

   albumin enhancer/promoter (Alb Mouse)
   

   Expression

   Mammalian

   Promoter

   Mouse Albumin Enhancer/Promoter

   Available Since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSP64T acvr1ba*

    Plasmid

    #185409

    Purpose

    Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    acvr1ba (acvr1ba Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Expression

    Mammalian

    Mutation

    changed Threonine 206 to Aspartic acid

    Available Since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid

    #68375

    Purpose

    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Promoter

    human PGK1

    Available Since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. CBF:H2B-Venus

    Plasmid

    #44211

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10

    Insert

    CBF1-Responsive-Element
    

    Expression

    Mammalian

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW-hSNCA-GFP

    Plasmid

    #215489

    Purpose

    Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

    Depositor

    Ophir Shalem

    Article

    Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX208 CMV sTurboID (C)-HA-FRB-ERM

    Plasmid

    #153007

    Purpose

    Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    sTurboID (C) (G74)-ERM (Cb5)
    

    Use

    Lentiviral

    Tags

    FRB and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5-HA-Ubiquitin-K63

    Plasmid

    #17606

    Purpose

    Mammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K63 only, other lysines mutated to arginines. Enh…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-Cre

    Plasmid

    #198712

    Purpose

    For lentiviral expression of Cre recombinase in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Insert

    Pgk-Cre
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEZYmyc-His

    Plasmid

    #18701

    Purpose

    pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Tags

    Myc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSGDlucV3.0

    Plasmid

    #119760

    Purpose

    dual luciferase with FMDV StopGo sequences flanking MCS

    Depositor

    John Atkins

    Article

    Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40 and T7

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-Ubiquitin-K48

    Plasmid

    #17605

    Purpose

    Mammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to arginines

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K48 only, other lysines mutated to arginines. Enh…

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-HA-Nrxn1beta AS4(+)

    Plasmid

    #59410

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(+) (Nrxn1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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