-
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25K
Plasmid#91147PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJKW0458
Plasmid#107587Purposep35S:CAS9DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterp35SAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCC155-MXMT
Plasmid#79816PurposeCFP-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsCC155/CFP (155–239)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB416
Plasmid#68681PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPSdown2
Plasmid#149419PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pABD1031
Plasmid#68004PurposeProtein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in plantaDepositorInsertPHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
UseTagseYFPExpressionPlantMutationPromoter2X Cauliflower Mosaic Virus 35S promoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:S10-(MCS)-3xHA
Plasmid#108183PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC4
Plasmid#91182PurposeT-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting 58kb region in medicago truncatula
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBRC169
Plasmid#79670PurposeDsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.DepositorTypeEmpty backboneUseReporter plasmidTagsDsRED-monomer (169–225)/RC169ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB2alpha2 35s:hCas9:tNos (GB0639)
Plasmid#68222PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoterDepositorInserthCas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ289-RVR
Plasmid#138127PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoterDepositorInsertErCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD513R, K519V and N523RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
TF101-STTM-miR156/157
Plasmid#84811PurposeTurning off/down miR156/157 in SoybeanDepositorInsertSTTM-miR156/157
UseTagsExpressionPlantMutationPromoterunknownAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB307
Plasmid#68635PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsCitrineExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.5
Plasmid#158407PurposeExpress single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22D
Plasmid#91136PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB409
Plasmid#68674PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS
Plasmid#123188Purposebinary plant vector for transient fLUC (with intron) expressionDepositorInsert2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDest_pK7WG2_pRPS5A_CTP OleGFP
Plasmid#171723PurposeptpTALECD vector assembly, Destination vector;DepositorInsertRPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 G10-90p
Plasmid#153370PurposeSynthetic promoter for plant expressionDepositorInsertG10-90 promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHMWGFPR
Plasmid#64396PurposeHigh molecular weight glutenin gene promoter driving GFP expressionDepositorInsertHigh molecular weight glutenin gene promoter
UseTagsGFPExpressionPlantMutationPromoterHigh molecular weight glutenin gene promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MIGS1.2 (JW640)
Plasmid#35246DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDGB3omega1_KanR_BastaR
Plasmid#186426Purposeoptimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)DepositorInsertsKanR
BastaR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterCaMV 35S and PnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre
Plasmid#192421PurposeTo act as the 2-input state of the OR gate.DepositorInsertAct2::FRT-loxP-OCS-FRT-loxP::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCJJ340
Plasmid#117502PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtUBI10Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM5026
Plasmid#84192Purposetarget miRNA5026 for destruction in Arabidopsis thalianaDepositorInsertSTTM5026
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB332
Plasmid#68660PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVR
Plasmid#138117PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoterDepositorInsertMb2Cas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V and N573RPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH80
Plasmid#128191PurposeLevel1 ZmUBIp::xCas9 3.7:NOStDepositorInsertZmUBIp::xCas9 3.7:NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH52
Plasmid#128181PurposeLevel1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOStDepositorInsert2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH85
Plasmid#128198Purposeclassic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)DepositorInsertclassic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB436
Plasmid#68701PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsTagRFPExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_102
Plasmid#91200PurposeTransformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: ToLCVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21D
Plasmid#91131PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB231
Plasmid#68622PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsEmerald Luc fused with PEST sequence [ELuc(PEST)]ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSS02:cyt-Peredox-mCherry
Plasmid#161743Purposecytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plantsDepositorInsertcyt-Peredox-mCherry
UseTagsExpressionPlantMutationPromoterUbiquitin 10Available sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_Venus_Blp
Plasmid#159704PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneUseTagsmVenusExpressionPlantMutationPromoterAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
MIGS3.2 (FF574)
Plasmid#35250DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM398
Plasmid#84124Purposetarget miRNA398 for destruction in Arabidopsis thalianaDepositorInsertSTTM398
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLXMA L2A
Plasmid#153360PurposeExpression vector for plants with medium-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL080
Plasmid#137889PurposeiSTU-CRISPR/Cas9 with OsCDPK2 intron, NU structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL085
Plasmid#137893PurposeiSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL083
Plasmid#137892PurposeiSTU-CRISPR/Cas9 with RcCat intron, NU structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 UBQ5t
Plasmid#153383PurposePlant terminatorDepositorInsertUBQ5 terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-D10A_5
Plasmid#91170PurposeCas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2151
Plasmid#141300PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneUseTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_263
Plasmid#91127PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL11023
Plasmid#117542PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S_OMEGA(pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM408
Plasmid#84132Purposetarget miRNA408 for destruction in Arabidopsis thalianaDepositorInsertSTTM408
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only