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Showing: 351 - 400 of 3935 results

  5. pH-ABE8e

    Plasmid
    #200774
    Purpose
    For plant adenine base editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038
    Insert
    nCas9(D10A)-TadA8e
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…
    Promoter
    maize Ubiquitin-1, OsU3
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200774-plasmid-map-sequence-id-399597

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  11. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  12. pGWG_RUBYseed

    Plasmid
    #225989
    Purpose
    To clone any gene of interest using gateway recombination cloning and the vector expresses seed-specific RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225989-plasmid-map-sequence-id-449367

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  13. dpYPQ230-D156R-CBE4

    Plasmid
    #195369
    Purpose
    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    hA3A/Y130F-(GGGGS)x6-dLbCas12a-D156R-UGI
    Use
    CRISPR; Gateway compatible ha3a/y130f-(ggggs)x6-d…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    ZmUBI1
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195369-plasmid-map-sequence-id-392981

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  17. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

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  20. pYUU

    Plasmid
    #159751
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159751-plasmid-map-sequence-id-313821

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  21. pENTR_AtU6p_BsaI_ccdb_gRNA

    Plasmid
    #225986
    Purpose
    To make Gateway entry clone of CRISPR guide RNA under AtU6p
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225986-plasmid-map-sequence-id-448026

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  26. dpYPQ230-D156R-ABE5

    Plasmid
    #195371
    Purpose
    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    ecTadA8e-(GGGGS)x8-dLbCas12a-D156R
    Use
    CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…
    Tags
    5' and 3' NLS
    Expression
    Plant
    Mutation
    Promoter
    ZmUBI1
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195371-plasmid-map-sequence-id-392986

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  30. pFH35

    Plasmid
    #126009
    Purpose
    Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    126009-plasmid-map-sequence-id-252792

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  31. pKPI-MtLAP1

    Plasmid
    #193521
    Purpose
    Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots
    Depositor
    Jeremy Murray
    Article
    Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np
    Inserts
    MtLAP1
    DsRed
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    ATUBI10 and Medtr8g059790(KPI)
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193521-plasmid-map-sequence-id-382387

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  34. pCBC-MT2T3

    Plasmid
    #50594
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50594-plasmid-map-sequence-id-75674

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  41. pTF101.1

    Plasmid
    #134770
    Purpose
    A binary plasmid containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.
    Depositor
    Kan Wang
    Article
    et al Euphytica (2004) 136: 167.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    134770-plasmid-map-sequence-id-263758

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  42. A3A-PBE

    Plasmid
    #119768
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-NLS-UGI-NLS
    Use
    Tags
    NLS (flanking UGI)
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A and E945K in wild type …
    Promoter
    maize Ubiquitin-1
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119768-plasmid-map-sequence-id-232090

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  47. pYPQ132-tRNA2.0

    Plasmid
    #158394
    Purpose
    Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158394-plasmid-map-sequence-id-330366

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  50. pMpGWB337

    Plasmid
    #68061
    Purpose
    Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter
    Depositor
    Takayuki Kohchi
    Article
    Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    Expression
    Plant
    Mutation
    Promoter
    MpEF1alpha promoter
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68061-plasmid-map-sequence-id-124390

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Showing: 351 - 400 of 3935 results