Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Worm Expression Clear filter

Applied Filters

Worm Expression Clear filter

1,779 results

Showing: 361 - 400 of 1779 results

  6. SP6-sgRNA-scaffold

    Plasmid
    #47912
    Purpose
    Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    sgRNA 3' end
    Expression
    Worm
    Promoter
    SP6
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47912-plasmid-map-sequence-id-68534

    Enlarge

  12. pJR021

    Plasmid
    #198814
    Purpose
    Variant of yellow fluorescent protein for transient gene expression
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::destabilizedYFP::let-838 3'UTR
    Expression
    Worm
    Mutation
    YFP with destabilized signal from mouse ornithine…
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198814-plasmid-map-sequence-id-391694

    Enlarge

  19. pCFJ210

    Plasmid
    #30538
    Depositor
    Erik Jorgensen
    Insert
    ttTi4348 region
    Expression
    Worm
    Available Since
    June 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    30538-plasmid-map-sequence-id-14100

    Enlarge

  20. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Article
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

    Enlarge

  25. pJH3449

    Plasmid
    #179576
    Purpose
    Pacr-5::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans B-MN/others and others expression of miniSOG-SL2 BFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Tags
    BFP
    Expression
    Worm
    Promoter
    Plgc-55B
    Available Since
    March 1, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    179576-plasmid-map-sequence-id-355663

    Enlarge

  27. pJW2072

    Plasmid
    #154334
    Purpose
    Crispr repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet-I (dpi)::3xMyc::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154334-plasmid-map-sequence-id-318659

    Enlarge

  35. pHW581

    Plasmid
    #198818
    Purpose
    In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
    Expression
    Worm
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198818-plasmid-map-sequence-id-391699

    Enlarge

  40. pJH4542

    Plasmid
    #200779
    Purpose
    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune
    Depositor
    Mei Zhen
    Article
    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do
    Insert
    GCaMP6
    Tags
    mNeptune
    Expression
    Worm
    Promoter
    Ptwk-40
    Available Since
    May 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    200779-plasmid-map-sequence-id-412786

    Enlarge

Showing: 361 - 400 of 1779 results