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8,350 results

Showing: 851 - 900 of 8350 results

  6. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  7. RPS1B-GFP (95)

    Plasmid
    #24039
    Depositor
    Michael Rout
    Insert
    RPS1B (RPS1B Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    March 31, 2010
    Availability
    Academic Institutions and Nonprofits only

  8. ZJOM160

    Plasmid
    #133670
    Purpose
    Integration of mTagBFP2-SKL as an additional copy in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast peroxisome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-mTagBFP2-SKL
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133670-plasmid-map-sequence-id-267532

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  13. pN3

    Plasmid
    #233520
    Purpose
    Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARS
    Depositor
    Carl Denard
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233520-plasmid-map-sequence-id-474154

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  16. pCAS9id

    Plasmid
    #141251
    Purpose
    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141251-plasmid-map-sequence-id-308879

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  19. pFS552

    Plasmid
    #242852
    Purpose
    This plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locus
    Depositor
    Nick Rhind
    Article
    Bashir et al MicroPubl Biol. 2025 Aug 21;2025:10.1791
    Inserts
    TetR
    mECitrine
    Expression
    Yeast
    Promoter
    CMV promoter and enotetSW1 promoter
    Available Since
    Sept. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242852-plasmid-map-sequence-id-485669

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  23. pCAS9cd

    Plasmid
    #141252
    Purpose
    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Article
    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    TEF1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141252-plasmid-map-sequence-id-308887

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  26. p404TDH3

    Plasmid
    #15973
    Depositor
    Nicolas Buchler, Fred Cross
    Insert
    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    TDH3 promoter is between SacI and XbaI restrictio…
    Available Since
    Nov. 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    15973-plasmid-map-sequence-id-8458

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  42. pUSE-URA3-Sec7-msYFP2

    Plasmid
    #218969
    Purpose
    Gene replacement plasmid to label S. cerevisiae Sec7 with msYFP2
    Depositor
    Benjamin Glick
    Insert
    SEC7-msYFP2 (SEC7 Budding Yeast)
    Tags
    msYFP2
    Expression
    Yeast
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218969-plasmid-map-sequence-id-445656

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  45. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  48. pCfB2197

    Plasmid
    #67551
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67551-plasmid-map-sequence-id-116386

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  49. pDEST-N2H-F2-empty vector

    Plasmid
    #125552
    Purpose
    N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2)
    Depositor
    Marc Vidal
    Article
    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:
    Type
    Empty backbone
    Use
    In vitro expression
    Tags
    NanoLuc fragment2 alone without any attached prot…
    Expression
    Mammalian and Yeast
    Promoter
    synthetic promoter containing yeast, mammalian, a…
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    125552-plasmid-map-sequence-id-252313

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  50. pNA0304

    Plasmid
    #165612
    Purpose
    Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    NotI site replaced the CAN1 spacer in parent vect…
    Promoter
    SNR52
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165612-plasmid-map-sequence-id-331070

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Showing: 851 - 900 of 8350 results