8,350 results
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Plasmid#171324PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertFirefly luciferase reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046381
Plasmid#149010PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationwtPromoterpTEF1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Vimentin/pAS2-1
Plasmid#98136PurposeVimentin (NM_003380) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertVimentin (VIM Human)
ExpressionYeastAvailable SinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-hphMX6-P41nmt1-3FLAG
Plasmid#19349DepositorInserthphMX6
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYC119
Plasmid#63916PurposeRecipient overexpressing vector to construct FLAG tagged fusionsDepositorTypeEmpty backboneTagsFLAGExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046692
Plasmid#149195PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
ExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
RPL25NLS-GFP (101)
Plasmid#24038DepositorInsertRPL25 (RPL25 Budding Yeast)
TagsEGFPExpressionYeastMutationOnly bp 1-135 (aa 1-45) of RPL25Available SinceMarch 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW Electra1-T2A-mCherry
Plasmid#205498PurposeExpression of Electra1-T2A-mCherry in yeastDepositorInsertElectra1-T2A-mCherry
ExpressionYeastAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFTK049
Plasmid#171321PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertmCherry fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -LEU- 4xFLAG
Plasmid#182527PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsert7 Copies of TetO Box
Tags4XFLAGExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBK51 pHomL-RPL18Bp-Neon(gfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)
Plasmid#179035PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SCS2tmh, a marker for the endoplasmic reticulumDepositorInsertRPL18Bp-Neon(gfp)-Link-SCS2tmh
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Spt2-GFP
Plasmid#115572PurposeExpresses yeast Spt2-GFP fusion proteinDepositorInsertSPT2
TagsGFPExpressionBacterial and YeastAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-Kan
Plasmid#44948PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selectionDepositorInsertyoPS-CFP2
ExpressionYeastAvailable SinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB1751
Plasmid#174013PurposepDUAL plasmid expressing GFP from 41nmt1 promoterDepositorInsertGFP
TagsGFPExpressionYeastPromoter41nmt1 and 41nmt1 promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW eGFP-T2A-mCherry
Plasmid#118422PurposeProduces equimolar expression of eGFP and mCherryDepositorInserteGFP-T2A-mCherry
ExpressionYeastAvailable SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-APL6-msGFPx3
Plasmid#105254PurposePlasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.DepositorAvailable SinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3050(gRNA XII-5)
Plasmid#73292PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSK260
Plasmid#129252Purposedual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporterDepositorInsertSup35 NM yTRAP sensor reporting on mKate2
ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHUM
Plasmid#64170Purposeharbours marker genes HIS3, URA3 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-EYFP
Plasmid#14214DepositorTypeEmpty backboneUseGateway CloningTagsEYFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXP732
Plasmid#46058DepositorTypeEmpty backboneExpressionYeastPromoterCUP1Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMG
Plasmid#138264PurposeYeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.DepositorInsertseGFP flanked by iCas9 sites
mCherry
UseCRISPRExpressionYeastPromoterTef1Available SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDZ514 pURA MET25p PP7-PS-2x-yeGFP
Plasmid#72230PurposeUracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoterDepositorInsertPP7-PS-2x-yeGFP
ExpressionYeastPromoterMET25Available SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1
Plasmid#182512PurposeC-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.DepositorInsertCL1 Degron
TagsGFPExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJZC625
Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
ExpressionYeastPromoterpAdhAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-HphMx
Plasmid#122895PurposeADE2-HphMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceOct. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOP461
Plasmid#85934PurposeEncodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.DepositorInsertAnti-Ebola 4G7 monoclonal antibody
ExpressionYeastPromoterAOX1Available SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG304GAL-ccdB-HA
Plasmid#14231DepositorTypeEmpty backboneUseGateway CloningTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAV0750
Plasmid#133484PurposeExpression in fission yeast from promoter purg1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(urg1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS191
Plasmid#34978DepositorInsertePDZb
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
YIp211-P30-His-POL30-3'
Plasmid#99546PurposeYeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 markerDepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNC824
Plasmid#20384DepositorInsertUAS cassette (FUS1)
ExpressionYeastMutationX=UbiY (see schematic)Available SinceMarch 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXP822
Plasmid#46064DepositorTypeEmpty backboneExpressionYeastPromoterGAL1Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LDB107
Plasmid#185423PurposeNUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporinDepositorInsertNUP1
TagsHAExpressionYeastAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pI2-MTU-DO-HIS
Plasmid#160016PurposeIntegrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSG830
Plasmid#158601PurposetNCS-ePTS1 (2micron)DepositorInsertpTDH3-tNCS-ePTS1
TagsePTS1ExpressionYeastAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFTK051
Plasmid#171323PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteBFP fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST BmOr2
Plasmid#73926PurposeUAS induced expression of Bombyx mori odorant receptor 2 (OR2) cDNADepositorInsertBmOr2
ExpressionYeastAvailable SinceMay 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
Plasmid#122067PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
TagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pWS1037
Plasmid#123049PurposeG_ subunitDepositorInsertGpa1 [3-4a]
ExpressionYeastAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS414 Hsh155 K410N
Plasmid#87280PurposeEncodes Hsh155 K410N for expression in yeastDepositorAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
KAP95-FLU (113)
Plasmid#24045DepositorAvailable SinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046574
Plasmid#148976PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
YIplac211-iGFP-YPT31
Plasmid#105263PurposePlasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS405 PEX14-VAC8-RFP
Plasmid#166844PurposeExpresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORFDepositorTagsPEX14 transmembrane domain and RFPExpressionYeastMutationPEX14 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
Plasmid#124278PurposeExpresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tagDepositorAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
ExpressionYeastPromoterpFUS1Available SinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only