8,265 results
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Plasmid#182534PurposeExpresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeastDepositorInsertAKAR3-EV-NR
ExpressionYeastMutationT506A mutationPromoterPMA1Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWS1837
Plasmid#123058Purpose_-factor pheromoneDepositorInsert_-factor [3-4a]
ExpressionYeastAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTCW002
Plasmid#83522Purposepheromone mediated destablised-GFP expression in yeastDepositorInsertpFUS1-yEGFPCLN2PEST
ExpressionYeastPromoterpFUS1Available SinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-NAT-Ago-Dcr
Plasmid#160381PurposeOverexpression of Ago-Dcr fusion protein in yeastDepositorInsertAgo- Dcr
ExpressionYeastPromoterGALAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT572_Nemo
Plasmid#153487PurposeLevel 1 episomal expression plasmid for S. cerevisiaeDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXP822
Plasmid#46064DepositorTypeEmpty backboneExpressionYeastPromoterGAL1Available SinceSept. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046574
Plasmid#148976PurposeYeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSD02
Plasmid#16009DepositorInsertACT1pr-DsRed.T1.N1
ExpressionYeastAvailable SinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
BB3aZ_14*
Plasmid#98542Purposeempty BB3 for insertion and overexpression of 1 gene in P. pastorisDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDS191
Plasmid#34978DepositorInsertePDZb
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMS22H
Plasmid#133904PurposeExpression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWHDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
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YIplac211-iGFP-YPT31
Plasmid#105263PurposePlasmid for pop-in/pop-out replacement of Ypt31 with iGFP-Ypt31.DepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETcon(I-LtrI)
Plasmid#41523DepositorInsertI-LtrI
Tags3x(G4S) Linker, G3S Linker, HA Tag, and Myc TagExpressionYeastPromoterGAL1Available SinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET2
Plasmid#107435PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-bleMX6-P81nmt1-3FLAG
Plasmid#19353DepositorInsertbleMX6
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVP64
Plasmid#22287DepositorAvailable SinceApril 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-mKO1-CaUra3
Plasmid#44638PurposeYeast tagging vector to create gene fusion with mKO1 with CaUra3 selectionDepositorInsertmKO1
ExpressionYeastAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGRE GFP
Plasmid#1196DepositorInsertGFP w/ stop codon
ExpressionYeastMutationGFP w/ stop codonAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
E2-Crimson-FKBP(L)
Plasmid#85001PurposeExpresses E2-Crimson-FKBP(L)DepositorInsertE2-Crimson-FKBP(L)
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-HAV_3C
Plasmid#203481PurposeExpresses HAV 3C protease from a GALL promoter with a URA3 markerDepositorInsertHAV 3C protease
ExpressionYeastPromoterGALLAvailable SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK372
Plasmid#73597PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-15 fatty acid desaturase
TagsFLAG tag and c-myc tagExpressionYeastPromoterGAL1 and GAL10Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046390
Plasmid#148996PurposeYeast Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceMay 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUDP003
Plasmid#101164PurposeE. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046457
Plasmid#145424PurposeYeast Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMEG_BB3_YL001
Plasmid#117825PurposeOverexpession plasmid of glycerol kinase (GUT1) in Y. lipolyticaDepositorInsertYlpTEF_YlGUT1_YlCyc1TT
ExpressionBacterial and YeastAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK234
Plasmid#131149Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2DepositorTypeEmpty backboneUseGateway destination vectorExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPKC1 (JCE575)
Plasmid#89773Purposeyeast expression of PKC1DepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC572
Plasmid#62318PurposesgRNA with 1x com for yeast cellsDepositorInsertsgRNA + 1x com RNA binding module
ExpressionYeastPromoterSNR52Available SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c3gRNA_RPR1t
Plasmid#64381Purposeencodes c3 gRNADepositorInsertc3 gRNA
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046332
Plasmid#145413PurposeYeast Expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTAL5-BB
Plasmid#36033DepositorTypeEmpty backboneExpressionYeastPromoterPGAL1Available SinceJuly 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-CPR
Plasmid#126742Purposeyeast plasmid overexpressing CPRDepositorInsertcpr
Tags6xHisExpressionYeastAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CUP Pan1 YFP p316
Plasmid#15592DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
EZ-L767
Plasmid#131773PurposePADH1_sFUS_Fusion Red_PixD_TACT1DepositorInsertPADH1_sFUS_Fusion Red_PixD_TACT1
UseIntegration into δ-sitesExpressionYeastPromoterPADH1Available SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-EYFP
Plasmid#14228DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS315-PGK-SEIPIN1
Plasmid#96986PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.DepositorInsertSEIPIN1 (AT5G16460 Mustard Weed)
ExpressionYeastPromoterPhosphoglycerate Kinase (PGK) promoterAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEmerald-Kan
Plasmid#44946PurposeYeast tagging vector to create gene fusion with yeast optimized Emerald with Kan selectionDepositorInsertyoEmerald
ExpressionYeastAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-SweetTrac1
Plasmid#169083PurposeExpression of SweetTrac1 biosensor in yeastDepositorInsertSweetTrac1
ExpressionYeastPromoterPMA1Available SinceFeb. 4, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBES2
Plasmid#83800PurposeUseful for tagging yeast proteins with N-terminal Venus tag; G418 selectionDepositorInsertVenus
ExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-P3nmt1-3FLAG
Plasmid#19348DepositorInserthphMX6
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCri-15a
Plasmid#61323PurposeProtein expression.DepositorInsertGreen fluorescent protein
TagsHis-tagExpressionYeastAvailable SinceJan. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZHY500
Plasmid#36186DepositorInsertFokI homodimeric Nuclease domain
TagsACV5ExpressionYeastMutationdelta152,+63 truncation to TAL backbonePromoterTEVAvailable SinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-CDS1-3HA
Plasmid#198596PurposeCDS1 overexpression. Diverts glycerolipid synthesis pathway towards phospholipids. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-DGK1-3HA
Plasmid#198595PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG75-ADH1-PAH1-3HA
Plasmid#198597PurposePAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
p416TEF-SEC16
Plasmid#102676PurposeThe gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretionDepositorAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only