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Showing: 351 - 400 of 7933 results

  3. pRN1120

    Plasmid
    #101750
    Purpose
    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.
    Depositor
    Rene Verwaal
    Article
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    101750-plasmid-map-sequence-id-199900

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  8. p415-GalL-Cas9-CYC1t

    Plasmid
    #43804
    Purpose
    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter
    Depositor
    George Church
    Article
    Dicarlo et al Nucleic Acids Res
    Insert
    Human Optimized S. pyogenes Cas9
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GalL
    Available since
    March 15, 2013
    Availability
    Academic Institutions and Nonprofits only
    43804-plasmid-map-sequence-id-59154

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  11. pCHAP3_Sap

    Plasmid
    #206849
    Purpose
    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206849-plasmid-map-sequence-id-419973

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  12. pCHAP2

    Plasmid
    #206847
    Purpose
    A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206847-plasmid-map-sequence-id-419939

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  13. pCHAP1

    Plasmid
    #206846
    Purpose
    A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206846-plasmid-map-sequence-id-419938

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  14. pE5n

    Plasmid
    #17459
    Purpose
    modified pENTR vector for N-terminal ECFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    ECFP
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17459-plasmid-map-sequence-id-8627

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  15. pV1524

    Plasmid
    #111431
    Purpose
    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)
    Depositor
    Gerald Fink
    Article
    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001
    Insert
    CaCas9/sgRNA-BsmBI stuffer
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111431-plasmid-map-sequence-id-292191

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  18. pDRF1 mBaojin-T2A-mCherry

    Plasmid
    #219842
    Purpose
    equimolar expression of mBaojin and mCherry in budding yeast
    Depositor
    Bas Teusink
    Insert
    mBaojin-T2A-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219842-plasmid-map-sequence-id-437589

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  23. pKL06

    Plasmid
    #104430
    Purpose
    super Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector
    Depositor
    Joel Kralj
    Article
    Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10
    Insert
    Super Ecliptic pHluorin-mRuby2
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    104430-plasmid-map-sequence-id-247766

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  24. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid
    #83239
    Purpose
    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.
    Depositor
    Tobias Dick
    Article
    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do
    Insert
    roGFP2-Tsa2dCpdCr
    Use
    Tags
    roGFP2 fusion with Tsa2dCpdCr
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    83239-plasmid-map-sequence-id-158262

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  30. pWS082

    Plasmid
    #90516
    Purpose
    sgRNA entry vector
    Depositor
    Tom Ellis
    Insert
    Yeast sgRNA expression vector
    Use
    CRISPR; Yeast gap repair
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    90516-plasmid-map-sequence-id-197186

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  39. pRS159

    Plasmid
    #122378
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122378-plasmid-map-sequence-id-241243

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  41. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    104910-plasmid-map-sequence-id-206061

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  45. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  46. pTH847-gst-optsc

    Plasmid
    #96908
    Purpose
    Plasmid for yeast expression of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    yeast codon optimised glutathione-S transferase
    Use
    Tags
    8xhistidine
    Expression
    Yeast
    Mutation
    Promoter
    TDH3
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    96908-plasmid-map-sequence-id-206073

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  47. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

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Showing: 351 - 400 of 7933 results