8,173 results
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Plasmid#160004PurposeLow-copy expression vector for K. marxianus, uracil selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusTagsn/aExpressionYeastPromotern/aAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only
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pTH727-CEN-RLuc/staCFLuc
Plasmid#38211DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYZ183
Plasmid#98425PurposePombe Expression Vector - nmt41 promoter with empty BsaI-pad - no markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ184
Plasmid#98426PurposePombe Expression Vector - nmt81 promoter with empty BsaI-pad - no markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMitoLOC
Plasmid#58980PurposeExpression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrilaDepositorInsertsmCherry
GFP
TagspreCOX4 and preSU9ExpressionYeastPromoterADH1 and MET17Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS220
Plasmid#34980PurposeExpresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.DepositorInsertePDZb–mCherry
ExpressionYeastPromoterTEFAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS314-URA3
Plasmid#11004DepositorAvailable SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIIIA/MS2-1
Plasmid#220631PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
ExpressionYeastAvailable SinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC-TALO8
Plasmid#83409PurposeTRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeastDepositorInsertTRP-ARS-8xLacO
ExpressionYeastMutationPlease see depositor comments belowPromoterTRP1Available SinceNov. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFG4
Plasmid#87251Purposegene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-HphDepositorInsertHyg
TagsparS segmentsExpressionYeastPromoterTEFAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSII42N-TDH3pr
Plasmid#194531PurposeHigh copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD100
Plasmid#212924PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSUC2 (SUC2 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUSE-URA3-Sec7-msYFP2
Plasmid#218969PurposeGene replacement plasmid to label S. cerevisiae Sec7 with msYFP2DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HC078 (3xHA-Atg13)
Plasmid#59544PurposeExpresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tagDepositorInsertATG13 (ATG13 Budding Yeast)
Tags3xHAExpressionBacterial and YeastMutationAvrII site created after start codonPromoterATG13Available SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pESC-leu2d(empty)
Plasmid#20120DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
EC2_10_dCas9_VPR_HC_sgRNA
Plasmid#163712PurposeYeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoSuperfolderGFP-Kan
Plasmid#44901PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with Kan selectionDepositorInsertyoSuperfolderGFP
ExpressionYeastAvailable SinceMay 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pLAT1_Cas9
Plasmid#104906PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceFeb. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDR-AmTrac
Plasmid#47770Purposeexpresses AmTrac in yeastDepositorInsertAmTrac
TagsmcpGFPExpressionYeastPromoterPMAAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pESC-TRP (V5 and HA)
Plasmid#222488PurposeCo-expression of two genes in yeast with V5 and HA tags and TRP1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOsTIR1w/oGFP
Plasmid#102883PurposeExpresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertOsTIR1
Tags3x mycExpressionYeastPromoterADH1Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
ExpressionYeastAvailable SinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS316-4M5.3
Plasmid#45831DepositorInsert4M5.3
TagsFLAGExpressionYeastPromoterGAL1Available SinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2195
Plasmid#67548PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBHM2675
Plasmid#241722PurposeIntegration of DNA into Cryptococcus neoformans Safe Haven 1 locus, contains ptxD marker for selection on media containing phosphite as a sole phosphorus sourceDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2619
Plasmid#234498PurposeamdS-CnU6-empty sgRNADepositorInsertamdS-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2662
Plasmid#234501PurposeLinker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-amdSDepositorInsertmNeonGreen-CBP-2XFLAG-amdS-PCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMK425 (GAL-OsTIR1(F74A))
Plasmid#140657PurposeGAL-OsTIR1(F74A)DepositorInsertGAL-OsTIR1(F74A)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKT0178
Plasmid#8744DepositorInserttdimer2
TagsCodon optimized linkerExpressionYeastAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P2
Plasmid#125035PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertPDC1 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG416Gal-GFP
Plasmid#38309DepositorInsertEGFP (eGFP )
ExpressionYeastAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197
Plasmid#67551PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#78231PurposeExpresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS305-Leu2-PINO4-PfV-GS-Sapphire
Plasmid#116930PurposeYeast integrative vector for the exression of the 40nm GEM under the INO4 promoterDepositorInsertPfv
TagsSapphireExpressionYeastPromoterINO4Available SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-EYFP
Plasmid#14219DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMEL13
Plasmid#107919Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP
Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS426 TEF mCherry
Plasmid#129433Purposeexpress mcherryDepositorInsertmcherry
ExpressionYeastAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEL12
Plasmid#107918Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB
Plasmid#14148DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pIGAhRC
Plasmid#112510Purposehuman Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoterDepositorUseSynthetic BiologyExpressionYeastPromoterGal1,10Available SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-FLAG-kanMX6
Plasmid#20751DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar1
Plasmid#232738PurposeExpresses NADPH/NADP+ biosensor NAPstar1 in S. cerevisiae.DepositorInsertNAPstar1
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T4
Plasmid#125056PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertPDC1 terminator
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJO672 (pRS415 Gal1-Ufd4-flag)
Plasmid#159590Purposeexpresses Ufd4-flag in yeast cellsDepositorInsertUfd4
ExpressionYeastAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
YCPlac33-sGFP-VRG4
Plasmid#25447DepositorInsertSynthetic Green Fluorescent Protein - VRG4
TagsGFPExpressionYeastAvailable SinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRG205MX
Plasmid#64535PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only