8,265 results
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Plasmid#205258PurposeS. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)DepositorInsertGtFz1
Tags10xHis, MBPExpressionYeastAvailable SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS316-IVa
Plasmid#31456DepositorInsertlinker
ExpressionYeastAvailable SinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-SpHis5
Plasmid#224076PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with SpHis5 selectionDepositorInsertymScarletI3
TagsymScarletI3ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSB819-PKR-hum
Plasmid#20030DepositorInsertProtein kinase R (EIF2AK2 Human)
ExpressionYeastAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
ExpressionYeastPromoterTetO7Available SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXNubA22-Dest
Plasmid#105087PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubAExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPZa-STE2-1D4
Plasmid#133422PurposeMutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.DepositorAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-HIS3MX6
Plasmid#227981PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertTurboID
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYES2-Ub-M-GFP
Plasmid#11952DepositorInsertUb-M-GFP
ExpressionYeastMutationUbiquitin fused to N-terminus of GFP. Methionine …Available SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAD
Plasmid#64747PurposeYeast expression plasmid: ACC1 (ser659ala, ser1157ala)DepositorInsertACC1 (ser659ala, ser1157ala)
ExpressionYeastAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Ptdh3-CAD-Ttdh1
Plasmid#127727PurposeYeast pathway position 3. CAD transcription unit with the TDH3 promoter and TDH1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyExpressionYeastPromoterPtdh3Available SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS416gT-GalL-Cas9
Plasmid#79903PurposepRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expressionDepositorInsertsCas9
guide RNA (gRNA)
Tetracycline Repressor
UseCRISPRExpressionYeastPromoterGPM1 promoter, GalL, and RPR1 promoter with TetO …Available SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl
Plasmid#70007PurposeCRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for sgRNA insertionDepositorInsertsCodon optimized Cas9 from S. pyogenes
sgRNA expression cassette
UseCRISPR and Synthetic BiologyExpressionYeastPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG424GAL-ccdB
Plasmid#14151DepositorTypeEmpty backboneUseGateway destinationExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ZJOM153
Plasmid#133642PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomCherry-CaURA3
Plasmid#44876PurposeYeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selectionDepositorInsertyomCherry
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HIP2
Plasmid#31436DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJJB1402
Plasmid#218616PurposeExpress Pex15, Pex22, Pex4, Pex1, and Pex6DepositorInsertPex15
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GFP11-His3MX
Plasmid#91951PurposeUniversal C-terminal GFP11 taggingDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNK5785
Plasmid#219753PurposepGAP-like vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of GAP promoter, for yeast expressionDepositorInsertpGAP - nnLuz_v4 - tAOX
UseLuciferaseExpressionYeastAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-TRP (V5 and HA)
Plasmid#222488PurposeCo-expression of two genes in yeast with V5 and HA tags and TRP1 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pWS3865
Plasmid#185742PurposeSub-array backbone 1/4DepositorTypeEmpty backboneExpressionYeastAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2403
Plasmid#171687PurposepRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.DepositorInsertCnoCAS9
UseCRISPRExpressionBacterial and YeastPromoterTEF1Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-SpHis5
Plasmid#125704PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selectionDepositorInsertymNeonGreen
TagsymNeonGreenExpressionYeastAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS267
Plasmid#122380PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRExpressionYeastAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-3xFLAG-kanMX6
Plasmid#20754DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTH727-CEN-RLuc/staCFLuc
Plasmid#38211DepositorInsertsFirefly Luciferase
Renilla luciferase
ExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available SinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAS9i
Plasmid#141249PurposeGalactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterGAL1 (Cas9), pSNR52 (gRNA)Available SinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3cN_pGAP_23*_pPFK300_Cas9
Plasmid#104911PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTCDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-HA
Plasmid#14243DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXNubWtgate
Plasmid#105080PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneTagsNubIExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCT2
Plasmid#130970PurposeHyPr plasmid with natMX markerDepositorInsertempty backbone
ExpressionYeastAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXNgate22-1HA
Plasmid#105077PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetOYC-Dest
Plasmid#105082PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloningDepositorTypeEmpty backboneTagsCub-PLV and mOST4ExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXNgate21-3HA
Plasmid#105078PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-ccdB-HA
Plasmid#14237DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-HIS3
Plasmid#229230PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHLUM
Plasmid#40276DepositorExpressionYeastPromoterendogenous LEU2, endogenous MET15/17, and endogen…Available SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
TagsFLAG tagExpressionYeastPromoterGAL10Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
ExpressionYeastMutationERG20 N127WAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-Kan
Plasmid#224077PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with Kan selectionDepositorInsertymScarletI3
TagsymScarletI3ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHRW32
Plasmid#130972PurposeHyPr plasmid with ble markerDepositorInsertempty backbone
ExpressionYeastAvailable SinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
LHZ1494
Plasmid#222104PurposeTriple sgRNAs expression plasmid in Kluyveromyces marxianus.DepositorInsertsg1RNA, sg4RNA, sg7RNA
UseCRISPRExpressionYeastAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pOBD2
Plasmid#196826PurposeYeast two-hybrid ; Gal4 DNA binding domainDepositorTypeEmpty backboneTagsGal4 DNA binding domainExpressionYeastPromoterADHAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
P415 ymBaojin
Plasmid#224086PurposeExpressing ymBaojin in yeastDepositorInsertymBaoJin
ExpressionYeastAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only