8,350 results
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Plasmid#45054DepositorInsertmtGFP
ExpressionYeastPromoterADHAvailable SinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCM189-ParB1::mCherry
Plasmid#87253PurposeExpresses ParB1-mCherry in yeast. Also known as pOR1-mchDepositorInsertParB1
TagsmCherryExpressionYeastPromoterpBAD/tetOAvailable SinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-BYG
Plasmid#158126PurposeDual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)DepositorInsertmTagBFP
ExpressionYeastPromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPAP001
Plasmid#153488PurposeLevel 1 episomal expression plasmid for P. pastorisDepositorTypeEmpty backboneExpressionYeastAvailable SinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR
Plasmid#24584DepositorInsertpADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188-PrARG8-GFP::URA3
Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeExpressionYeastPromoterARG8Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeExpressionYeastPromoterGNP1Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFG2
Plasmid#87250Purposegene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-NatDepositorInsertNat
TagsparS segmentsExpressionYeastPromoterTEFAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTDH3-dCas9-Mxi1
Plasmid#46921PurposeYeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoterDepositorInsertdCas9-Mxi1
UseCRISPRExpressionYeastPromoterTDH3Available SinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
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ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
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ZJOM140
Plasmid#133668PurposeIntegration of COX4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.DepositorAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPMVAu5 (R)
Plasmid#98296PurposeEnhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
ExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRExpressionYeastPromoterGalLAvailable SinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ccdB
Plasmid#14154DepositorTypeEmpty backboneUseGateway CloningExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastPromoterGPDAvailable SinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-link-ymTq2-URA3
Plasmid#168057PurposeReplaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymTurquoise2
TagsymTurquoise2ExpressionYeastAvailable SinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW2 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS143
Plasmid#122377PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PP74
Plasmid#78944PurposeSupplemental Figure 3. Integration Site 1.DepositorInsertIntegration Site 1
ExpressionYeastAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415-PtrpC-Cas9-TtrpC-CYC1t
Plasmid#68059PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.DepositorInserthumanized Cas9
UseCRISPR; N. crassa, fungiExpressionYeastAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXN21-Dest
Plasmid#105086PurposeYeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX142-mtGFP
Plasmid#45050DepositorInsertmtGFP
ExpressionYeastPromoterTPIAvailable SinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS118
Plasmid#234878PurposeCRISPR-Cas9 plasmid for use in Candida albicans. Contains Cas9, NEUT5L integration site, and the sgRNA cloning site (SNR52 promoter)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastPromoternoAvailable SinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGKB
Plasmid#85077Purposeexpression in Pichia pastorisDepositorTypeEmpty backboneExpressionYeastPromoterGAPAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB
Plasmid#14133DepositorTypeEmpty backboneUseGateway CloningExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Tgl3-4V5-APEX2-URA
Plasmid#207066PurposeExpressing Tgl3-4V5-APEX2. Marker for lipid droplets.DepositorInsertTgl3
Tags4xV5-APEX2ExpressionYeastPromoterpADH3Available SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSK450
Plasmid#34552DepositorInsertMS2-CP-NLS
TagsmCherryExpressionYeastPromoterMet25Available SinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZJOM136
Plasmid#133660PurposeIntegration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB2960
Plasmid#8677DepositorAvailable SinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP2_leu2d_NRT1.1
Plasmid#58332PurposeExpresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged proteinDepositorAvailable SinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
FRP2370
Plasmid#127576PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS1244
Plasmid#247644PurposeInducible CRISPRi-HyperdCas12a for Candida albicansDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS1074
Plasmid#247642PurposeConstitutive CRISPRi-HyperdCas12a for Candida albicansDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRS1242
Plasmid#247643PurposeInducible CRISPRa-HyperdCas12a for Candida albicansDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pRB2963
Plasmid#8678DepositorAvailable SinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-3HA-HIS3MX6
Plasmid#228025PurposeTo integrate TID-FKBP fusion at yeast promoter; yeast BioID with HIS3MX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pML107-PMR1
Plasmid#226264PurposePlasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 geneDepositorAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
UG75-DGK1-3HA
Plasmid#184759PurposeDGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.DepositorAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG76-pLYS2-GFP-PKCbeta
Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1667
Plasmid#112041PurposeCEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URADepositorInsertERG5
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3
Plasmid#188064PurposePROSS-designed high-redox potential laccase from Trametes hirsutaDepositorInsertPROSS-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 mutations, described in publicationAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA-VRC01-scFv
Plasmid#44874DepositorInsertVRC01 scFv
TagsHA and MycExpressionYeastPromoterGAL1Available SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomCherry-Kan
Plasmid#44903PurposeYeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selectionDepositorInsertyomCherry
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pESC-leu2d(empty)
Plasmid#20120DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceFeb. 12, 2009AvailabilityAcademic Institutions and Nonprofits only