We narrowed to 20,236 results for: Sele
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Plasmid#13029DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertELAM (SELE Human)
UseLuciferaseTagsLucExpressionMammalianAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pD649-HAsp-SELE-Fc(DAPA)-AviTag-6xHis
Plasmid#156908PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSELE (SELE Human)
TagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SELE-COMP5AP-AviTag-9xHis
Plasmid#157472PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA122 - peel-1 negative selection
Plasmid#34873PurposeNegative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegansDepositorInsertpeel-1 (peel-1 Nematode)
UseWorm targetingAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGGAselect
Plasmid#195714PurposepGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.DepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceFeb. 24, 2023AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRExpressionInsectPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-frame_selector_1
Plasmid#127554PurposeDrosophila expression of frame selector sgRNA 1 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 1
UseCRISPRExpressionInsectPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-frame_selector_0
Plasmid#127553PurposeDrosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 0
UseCRISPRExpressionInsectPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-frame_selector_2
Plasmid#127555PurposeDrosophila expression of frame selector sgRNA 2 that targets CRISPaint site for homology-independent knock-inDepositorInsertsgRNA frame 2
UseCRISPRExpressionInsectPromoterdU6:3Available sinceJuly 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSELECT-HA-mFOXO1
Plasmid#83308PurposeExpresses HA-tagged mouse FoxO1 in mammalian cells, PuroR selection markerDepositorAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
env8 selectivity (b')
Plasmid#99837PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.DepositorInsertenv8 selectivity (b')
TagsmNeonGreenExpressionBacterialMutationA20G, A68G, G-C pairs in P1 and P3Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSelect-6
Plasmid#190243PurposeEncodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.DepositorInsertLacI
ExpressionBacterialAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP
Plasmid#13031PurposeMammalian expression vector for cloning your gene fused to EGFPDepositorInsertEnhanced Green Fluorescent Protein
ExpressionMammalianAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSelect-2
Plasmid#190242PurposeEncodes lacI-targeting gRNA for positive selection of dCas9 activity.DepositorInsertLacI
ExpressionBacterialAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
env8 selectivity (d')
Plasmid#99838PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.DepositorInsertenv8 selectivity (d')
TagsmNeonGreenExpressionBacterialMutationA20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-Ubiquitin
Plasmid#18712PurposeMammalian expression of human ubiquitin with HA tagDepositorInsertubiquitin (UBB Human)
TagsHAExpressionMammalianMutationThe HA-Ubiquitin insert is from the UBB gene. The…Available sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SP-dCas9-VPR
Plasmid#63798PurposeSP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSP-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionMammalianPromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-GCaMP6s
Plasmid#40753PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP6s
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ptfLC3
Plasmid#21074PurposeMammalian expression of rat LC3 fused to mRFP and EGFPDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
TagsEGFP and mRFPExpressionMammalianAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorTypeEmpty backboneUseLentiviralAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBirA(R118G)-HA
TagsHAExpressionMammalianMutationR118G (highly promiscuous form)Available sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorTypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 mycBioID
Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBirA
TagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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myc-BioID2-MCS
Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorTypeEmpty backboneTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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human beta-catenin pcDNA3
Plasmid#16828PurposeMammalian expression of human beta-catenin with Flag tagDepositorAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-CMV-GCaMP6f
Plasmid#40755PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGCaMP6f
Tags6xHis, T7 epitope, and Xpress tagExpressionMammalianPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-HIF1alpha-pcDNA3
Plasmid#18949DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHypoxia inducible factor 1 alpha (HIF1A Human)
TagsHA-tagExpressionMammalianMutationwt cDNAAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H2B-mCherry
Plasmid#20972PurposeH2B fused to mCherry for chromatin visualizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHistone cluster 1, H2bb (H2BC3 Human)
TagsmCherryExpressionMammalianAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by