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Showing: 1 - 30 of 22126 results

  12. env8 selectivity (b')

    Plasmid
    #99837
    Purpose
    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7
    Insert
    env8 selectivity (b')
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    A20G, A68G, G-C pairs in P1 and P3
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    99837-plasmid-map-sequence-id-192118

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  13. env8 selectivity (d')

    Plasmid
    #99838
    Purpose
    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
    Depositor
    Robert Batey
    Article
    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7
    Insert
    env8 selectivity (d')
    Use
    Tags
    mNeonGreen
    Expression
    Bacterial
    Mutation
    A20G, A68G, G-C pairs in P1 and P3, G12A, G10U de…
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    99838-plasmid-map-sequence-id-192115

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  17. pSELECT-HA-mFOXO1-D256

    Plasmid
    #83379
    Purpose
    expresses mouse FOXO1 with truncating mutation at AA 256 in mammalian cells
    Depositor
    Steven Abcouwer
    Insert
    Forkhead box O1 (Foxo1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    N256TAG (premature stop codon)
    Promoter
    EF-1a
    Available since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pD649-HAsp-SELE-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156908
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SELE (SELE Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156908-plasmid-map-sequence-id-444386

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  22. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  23. pGL3-ELAM-luc

    Plasmid
    #13029
    Depositor
    Doug Golenbock
    Insert
    ELAM (SELE Human)
    Use
    Luciferase
    Tags
    Luc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13029-plasmid-map-sequence-id-469496

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  24. LUC56 (Luc/UGA/SP)

    Plasmid
    #112147
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Cysteine 258mutated to selenocysteine (U)
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112147-plasmid-map-sequence-id-214894

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  25. pSecUAG-Evol2

    Plasmid
    #163148
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons
    Depositor
    Dieter Söll
    Article
    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2
    Inserts
    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG), Cys…
    Promoter
    EM7, araBAD, native As SelD promoter, and native …
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    163148-plasmid-map-sequence-id-331409

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Showing: 1 - 30 of 22126 results