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We narrowed to 557 results for: HEXA

Showing: 1 - 50 of 557 results

  1. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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  2. A1-LCD deltahexa

    Plasmid
    #169714
    Purpose
    Bacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deleted
    Depositor
    Tanja Mittag
    Article
    Martin et al Science. 2020 Feb 7;367(6478):694-699. d
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    hnRNPA1-A Low complexity domain including amino a…
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169714-plasmid-map-sequence-id-334793

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  3. SARS-CoV-2 S HexaPro

    Plasmid
    #154754
    Purpose
    Mammalian expression vector for expression of the SARS-CoV-2 spike HexaPro variant
    Depositor
    Jason McLellan
    Article
    Hsieh et al Science. 2020 Sep 18;369(6510):1501-1505
    Insert
    Spike (HexaPro variant) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    Available since
    July 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154754-plasmid-map-sequence-id-304640

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  4. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

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  10. SARS CoV-2 HexaPro spike (bio-His)

    Plasmid
    #166856
    Purpose
    Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.
    Depositor
    Evelien Bunnik
    Article
    Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d
    Insert
    SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)
    Use
    Tags
    8xHis and Biotinylation site
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    166856-plasmid-map-sequence-id-327668

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  11. EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

    Plasmid
    #194234
    Purpose
    Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker
    Depositor
    Hana El-Samad
    Article
    Weinberg et al bioRxiv 2021.04.20.440678
    Insert
    EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    194234-plasmid-map-sequence-id-383907

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  12. OmpA3-PNGaseF-TEV-His6

    Plasmid
    #114274
    Purpose
    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.
    Depositor
    Martin Caffrey
    Insert
    Peptide: N-glycosidase F
    Use
    Tags
    OmpA3 leader sequence (for periplasmic localisati…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114274-plasmid-map-sequence-id-218322

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  13. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

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  14. p28BIOH-LIC

    Plasmid
    #62352
    Purpose
    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62352-plasmid-map-sequence-id-102272

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  15. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pPP085

    Plasmid
    #158795
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158795-plasmid-map-sequence-id-309510

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  24. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  26. pPP089

    Plasmid
    #158796
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158796-plasmid-map-sequence-id-309513

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  27. pPP076

    Plasmid
    #158794
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158794-plasmid-map-sequence-id-309576

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  29. His-NavMs

    Plasmid
    #100004
    Purpose
    Electrophysiology in mammalian cells: Mammalian expression of voltage gated sodium channel and GFP
    Depositor
    Paul DeCaen
    Article
    Sula et al Nat Commun. 2017 Feb 16;8:14205. doi: 10
    Insert
    His-NavMs, Voltage gated sodium channel
    Use
    Tags
    Hexa-His tag followed by a thrombin cleavage site
    Expression
    Mammalian
    Mutation
    C-terminus from a codon-optimized synthetic gene,…
    Promoter
    CMV
    Available since
    Aug. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100004-plasmid-map-sequence-id-192452

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  30. pPP378

    Plasmid
    #158797
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-1 (D371A)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    158797-plasmid-map-sequence-id-309517

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  31. pPP384

    Plasmid
    #158799
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-3 (D413A)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158799-plasmid-map-sequence-id-309569

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  35. pBN089

    Plasmid
    #173451
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173451-plasmid-map-sequence-id-344804

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  38. pBN083

    Plasmid
    #173445
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y705E, Y706E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y705E, Y706E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173445-plasmid-map-sequence-id-344777

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Showing: 1 - 50 of 557 results