Skip to main content
Addgene
Showing: 1 - 50 of 333 results

  1. DNA-CARtcr-IRESpuro

    Plasmid
    #89346
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
    Depositor
    Ron Vale
    Article
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    Use
    Lentiviral
    Tags
    SNAPf tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89346-plasmid-map-sequence-id-176306

    Enlarge

  6. GFP.Cre empty vector

    Plasmid
    #20781
    Depositor
    Tyler Jacks
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2009
    Availability
    Academic Institutions and Nonprofits only
    20781-plasmid-map-sequence-id-213372

    Enlarge

  11. pdCas9-sgRNA-com-BoxB-vector

    Plasmid
    #132556
    Purpose
    Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.
    Depositor
    Baisong Lu
    Inserts
    dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
    Lambda N22-KRAB
    sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    132556-plasmid-map-sequence-id-261537

    Enlarge

  14. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

    Enlarge

  15. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224898-plasmid-map-sequence-id-443622

    Enlarge

  17. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA
    Available since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24150-plasmid-map-sequence-id-376525

    Enlarge

  18. psPAX2-D64V

    Plasmid
    #63586
    Purpose
    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.
    Depositor
    Andrew Scharenberg, David Rawlings
    Article
    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi
    Insert
    Packaging plasmid for integrase deficient lentiviral vectors
    Use
    Integrase deficient lentiviral packaging vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    63586-plasmid-map-sequence-id-464218

    Enlarge

  19. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid
    #200029
    Purpose
    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    PuroR-P2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200029-plasmid-map-sequence-id-393200

    Enlarge

  21. pWPI

    Plasmid
    #12254
    Purpose
    2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.
    Depositor
    Didier Trono
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox and Lentiviral
    Tags
    EMCV IRES-EGFP cassette
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12254-plasmid-map-sequence-id-429661

    Enlarge

  22. pLV-SFFV-Emerald-WPRE-UbC-mCherry

    Plasmid
    #211802
    Purpose
    Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    Emerald
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211802-plasmid-map-sequence-id-418109

    Enlarge

  23. pLV-SFFV-mCherry-WPRE-UbC-Emerald

    Plasmid
    #211797
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    mCherry
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211797-plasmid-map-sequence-id-418065

    Enlarge

  24. pLV-SFFV-RAB10-WPRE-UbC-Emerald

    Plasmid
    #203804
    Purpose
    Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    RAB10 (RAB10 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203804-plasmid-map-sequence-id-401585

    Enlarge

  25. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid
    #203805
    Purpose
    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    Llgl1 (Llgl1 Mouse)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203805-plasmid-map-sequence-id-401577

    Enlarge

  27. pLV-SFFV-MCS-WPRE-UbC-mCherry

    Plasmid
    #211799
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    multiple cloning site
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211799-plasmid-map-sequence-id-418073

    Enlarge

  28. pLV-SFFV-mCherry-WPRE-UbC-MCS

    Plasmid
    #211801
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    mCherry
    multiple cloning site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211801-plasmid-map-sequence-id-418105

    Enlarge

  29. pLV-SFFV-MCS-WPRE-UbC-Emerald

    Plasmid
    #211796
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    multiple cloning site
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211796-plasmid-map-sequence-id-418059

    Enlarge

  30. pLV-SFFV-Emerald-WPRE-UbC-MCS

    Plasmid
    #211800
    Purpose
    Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    Emerald
    multiple cloning site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211800-plasmid-map-sequence-id-418102

    Enlarge

  31. pLV-SFFV-MCS-WPRE-UbC-MCS

    Plasmid
    #211798
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and another multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    multiple cloning site
    multiple cloning site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211798-plasmid-map-sequence-id-418070

    Enlarge

  32. pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald

    Plasmid
    #203803
    Purpose
    Lentiviral vector plasmid expressing human RAB10 mutation Q68L (constitutively active mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    RAB10 (RAB10 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q68L
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203803-plasmid-map-sequence-id-401592

    Enlarge

  33. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

    Plasmid
    #203802
    Purpose
    Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    RAB10 (RAB10 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T23N
    Promoter
    Available since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    203802-plasmid-map-sequence-id-401645

    Enlarge

  36. pLEX_307

    Plasmid
    #41392
    Purpose
    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41392-plasmid-map-sequence-id-331248

    Enlarge

  37. pGAE-UFO.664

    Plasmid
    #183471
    Purpose
    Lentiviral vector expressing HIV Envelope UFO.664
    Depositor
    Andrea Cara
    Insert
    HIV envelope
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183471-plasmid-map-sequence-id-360575

    Enlarge

  38. pGAE-UFO.750

    Plasmid
    #183472
    Purpose
    Lentiviral vector expressing HIV Envelope UFO.750
    Depositor
    Andrea Cara
    Insert
    HIV Envelope
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183472-plasmid-map-sequence-id-360574

    Enlarge

  42. pCW57.1

    Plasmid
    #41393
    Purpose
    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41393-plasmid-map-sequence-id-364440

    Enlarge

  43. pLIX_403

    Plasmid
    #41395
    Purpose
    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA
    Depositor
    David Root
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector, doxycycli…
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41395-plasmid-map-sequence-id-364439

    Enlarge

  44. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid
    #89180
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89180-plasmid-map-sequence-id-176308

    Enlarge

  45. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

    Enlarge

  46. pDual_dsCas9_Venus_hs_NC_chr1

    Plasmid
    #214682
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214682-plasmid-map-sequence-id-428816

    Enlarge

  47. pDual_dsCas9_Venus_hs_NC_chr2

    Plasmid
    #214683
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214683-plasmid-map-sequence-id-428804

    Enlarge

  48. pDual_dsCas9_BFP_hs_NC_chr1

    Plasmid
    #214686
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214686-plasmid-map-sequence-id-428810

    Enlarge

Showing: 1 - 50 of 333 results