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3,641 results

Showing: 1 - 50 of 3641 results

  4. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154786-plasmid-map-sequence-id-306310

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  14. pAc5.1B-EGFP-DmG3BP_G

    Plasmid
    #146349
    Purpose
    Insect Expression of DmG3BP
    Depositor
    Elisa Izaurralde
    Insert
    DmG3BP (rin Fly)
    Expression
    Insect
    Mutation
    V632G compared to AF231031.1, exact match to NP_5…
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146349-plasmid-map-sequence-id-396887

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  24. pCFD3.1-w-dU6:3gRNA

    Plasmid
    #123366
    Purpose
    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).
    Depositor
    Simon Bullock
    Insert
    U6:3-gRNA
    Use
    CRISPR
    Expression
    Insect
    Available Since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    123366-plasmid-map-sequence-id-242548

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  26. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Expression
    Insect
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  36. pUASp YFP Cdc42 CA

    Plasmid
    #52250
    Purpose
    Drosophila expression of YFP-Cdc42 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52250-plasmid-map-sequence-id-499287

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  37. pUASp YFP Cdc42 WT

    Plasmid
    #52248
    Purpose
    Drosophila expression of YFP-Cdc42
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    52248-plasmid-map-sequence-id-499288

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  38. pAc.1B-EGFP-DmHrb98

    Plasmid
    #66595
    Purpose
    Expresses EGFP-tagged Hrb98 in Drosophila cells
    Depositor
    Elisa Izaurralde
    Insert
    Hrb98 (Hrb98DE Fly)
    Use
    Drosophila expression
    Tags
    EGFP
    Promoter
    Actin 5C
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66595-plasmid-map-sequence-id-113871

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  43. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154787-plasmid-map-sequence-id-306329

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  46. pUC57-white[coffee]

    Plasmid
    #84006
    Purpose
    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
    Depositor
    Phillip Zamore
    Article
    Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.
    Insert
    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    Use
    Unspecified
    Mutation
    A GC-to-AA mutation that creates a G589E missense…
    Available Since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    84006-plasmid-map-sequence-id-165529

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  49. pHD-DsRed-attP-w+

    Plasmid
    #80898
    Purpose
    Vector for generating dsDNA donors for HDR. Contains an attP docking site, the visible marker 3xP3-DsRed and GMR-white for identifying backbone incorporation.
    Depositor
    Kate O'Connor-Giles
    Inserts
    attP
    LoxP-3xP3-DsRed-LoxP
    GMR-white
    Use
    High copy, amp resistance
    Promoter
    3xP3 and GMR::hsp70
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    80898-plasmid-map-sequence-id-172486

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Showing: 1 - 50 of 3641 results