12,913 results

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pCDNA3 Flag MKK7b1

Plasmid

#14622

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1 (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
Beta4-spectrin scFv [N393/2]

Plasmid

#190550

Purpose

Mammalian Expression of Beta4-spectrin scFV. Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Beta4-spectrin (Homo sapiens) recombinant scFV (SPTBN4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Cbx1-V5

Plasmid

#175106

Purpose

Lentiviral expression of mouse Cbx1-V5

Depositor

Larry Gerace

Insert

Cbx1 (Cbx1 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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mEos4b-MannII-N-10

Plasmid

#57513

Purpose

Localization: Mannosidase/Golgi, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

MannII (Man2a1 Mouse)

Tags

mEos4b

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)mGAT1YFP3

Plasmid

#41667

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; 3 C-terminal-m…

Promoter

CMV

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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GwKif2CGFP

Plasmid

#21479

Purpose

Expression of KifC2-GFP in mammalian cells.

Depositor

Don Arnold

Article

Chu et al J Biol Chem. 2006 Jan 6. 281(1):365-73.

Insert

Kinesin family member C2 (Kifc2 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

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Hoxb7 promoter

Plasmid

#21294

Depositor

Frank Costantini

Article

Srinivas et al Development. 1999 Apr . 126(7):1375-86.

Insert

Hoxb7 promoter (Hoxb7 Mouse)

Use

Promoter can be used to make transgenic mice

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
isoform PKC Iota (JCE597)

Plasmid

#182030

Purpose

yeast expression of mouse PKC Iota with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Insert

PKC Iota (Prkci Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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GST-PRDM16 1-223

Plasmid

#53346

Purpose

bacterial expression of mouse PRDM16 fragment

Depositor

Shingo Kajimura, Bruce Spiegelman

Article

Kajimura et al Genes Dev. 2008 May 15;22(10):1397-409.

Insert

PRDM16 (Prdm16 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 1-223

Promoter

tac

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only
pLV-shEsr2

Plasmid

#120722

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

Depositor

Amy Lasek

Article

Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

Insert

Esr2 (Esr2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId3sp

Plasmid

#16045

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id3 (Id3 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 365-635

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
Anti-HCN4 [N114/10R-2b]

Plasmid

#206653

Purpose

Mammalian Expression Plasmid of anti-HCN4 (Human). Derived from hybridoma N114/10-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN4 (Homo sapiens) recombinant Mouse monoclonal antibody (HCN4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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HCN4 scFv [N114/10]

Plasmid

#190526

Purpose

Mammalian Expression of HCN4 scFV. Derived from hybridoma N114/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

HCN4 (Homo sapiens) recombinant scFV (HCN4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pKmyc-Borg3

Plasmid

#141074

Purpose

Expresses Borg3 (Cdc42ep5) in mammalian cells

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol. 2001 Oct;3(10):861-6. doi

Insert

Borg3 (Cdc42ep5 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN

Plasmid

#178032

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Promoter

T7LacI

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac flag Bmi1

Plasmid

#1967

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Bmi1 (Bmi1 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
px330 p300 gRNA

Plasmid

#165591

Purpose

Insertion of p300 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

P300 (Smarca2 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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D28 Cortactin GFP

Plasmid

#26723

Depositor

Anna Huttenlocher

Article

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion removed 28 amino acids (aa# 334-361) enc…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pINTO-NSA::mEzh2

Plasmid

#61725

Purpose

Expression of mouse Ezh2 fused to monomeric streptavidin (N-terminus)

Depositor

Roberto Bonasio

Article

Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

Insert

Enhancer of zeste homolog 2 (Ezh2 Mouse)

Tags

mSA (monomeric streptavidin)

Expression

Mammalian

Promoter

T-REx (CMV + 2xTetO2)

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-GAP43-ECFP-stop-L2

Plasmid

#186356

Purpose

Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Growth-associated protein-43 (Gap43 Mouse, Synthetic)

Use

Expression of a fluorescent membrane marker

Tags

ECFP

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-Brn2-DBD-EnR

Plasmid

#19714

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Fly engrailed 36240 (Pou3f2, en Mouse, Fly)

Expression

Mammalian

Mutation

mouse Brn2 DNA binding domain-Drosophila engraile…

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pR26-mBMP7

Plasmid

#127374

Purpose

Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

BMP7 (Bmp7 Mouse)

Use

CRISPR and Mouse Targeting

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/38R-1]

Plasmid

#206719

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/38-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant Mouse monoclonal antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCALNL-Kir2.1

Plasmid

#125578

Purpose

Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Eomes

Plasmid

#128825

Purpose

dox-dependent expression of mouse Eomes

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Eomes (Eomes Mouse)

Use

Lentiviral

Mutation

Shorter isoform

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1

Plasmid

#58415

Purpose

Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pET28a-FKBP-STING(pLxIS)

Plasmid

#221333

Purpose

Overexpression of STING CTT in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-MyosinIIC-N-18

Plasmid

#55108

Purpose

Localization: Cytoskeleton, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Ebrahim et al Curr Biol. 2013 Apr 22;23(8):731-6. doi:

Insert

MyosinIIC

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
Halo-Sox13

Plasmid

#200194

Purpose

Expression of full length Sox13

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-Sox13 (Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-iRFP670

Plasmid

#197202

Purpose

Expresses the protein of iRFP670 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP670

Use

AAV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/mArf3(T31N)-HA

Plasmid

#79416

Purpose

Expresses C-terminally HA-tagged Arf3(T31N) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 31 to Asparagine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3/mArf3(Q71L)-HA

Plasmid

#79415

Purpose

Expresses C-terminally HA-tagged Arf3(Q71L) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Kondo et al Cell Struct Funct. 2012;37(2):141-54. Ep

Insert

Arf3 (Arf3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamine 71 to Leucine

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-H19-luciferase ME

Plasmid

#26613

Depositor

Jie Chen

Article

Erbay et al J Cell Biol. 2003 Dec 8. 163(5):931-6.

Insert

Igf2 enhancer

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
3538 pMIG Bad

Plasmid

#8787

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bad (Bad Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2005

Availability

Academic Institutions and Nonprofits only
FLAG-KIF5A tail only

Plasmid

#166956

Purpose

Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A (820-1027aa)

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-ATG3-C-18

Plasmid

#54993

Purpose

Localization: Membrane Receptor, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

ATG3 (ATG3 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393

Plasmid

#216380

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

3C-splitGFP1to10opt-ETVV and HA signal sequence-F…

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV hSynapsin SV-tag

Plasmid

#163685

Purpose

express SV-Tag construct in a Cre-independent manner

Depositor

Bernardo Sabatini

Article

Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

Insert

synatophysin 9X HA tag T2A tdtomato

Use

AAV

Tags

9X HA

Expression

Mammalian

Promoter

synapsin

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgHcn1#2/Cre

Plasmid

#193218

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgHcn1#2 (Hcn1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MIG-eIF4A1 P159E

Plasmid

#70042

Purpose

Expression of eIF4A1 P159E mutant construct in mammalian cells.

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

eIF4A1 (Eif4a1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

P159E

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only
pSicoR-mCh-Oct4i

Plasmid

#21906

Depositor

Miguel Ramalho-Santos

Article

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

Oct4i

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Ssh1

Plasmid

#163602

Purpose

Expresses Protein phosphatase Slingshot homolog 1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

ssh1 (Ssh1 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-mFzd1-1D4

Plasmid

#42263

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 1 (Fzd1 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pLINE1-scFvFc

Plasmid

#206020

Purpose

An intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP/Venus-mULK1

Plasmid

#58743

Purpose

Expresses Venus-mULK1 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

Venus

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pSH1/M-FGFR3-Fv-Fvls-E

Plasmid

#15287

Depositor

David Spencer

Article

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Insert

FGFR3 kinase, FKBP12v36 (Fgfr3 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR3 cytoplasmic domain only FKBP12 F36V

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD-CMV-STAT5B-DN-CMV-GFP

Plasmid

#83262

Purpose

Adenoviral expression of dominant negative STAT5B with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5b (Stat5b Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative (Y699F)

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV Flag dPDZ-PTEN (1-400)

Plasmid

#22232

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

Insert

PTEN (Pten Mouse)

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

PDZ binding domain deleted. Contains amino acids …

Available Since

March 19, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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