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19,771 results

Showing: 3001 - 3030 of 19771 results

  1. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71685-plasmid-map-sequence-id-322543

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  9. pTREtight2

    Plasmid
    #19407
    Depositor
    Markus Ralser
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    Oct. 15, 2008
    Availability
    Academic Institutions and Nonprofits only
    19407-plasmid-map-sequence-id-181154

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  10. pCuo CA Rac1

    Plasmid
    #84643
    Purpose
    constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.
    Depositor
    Sanjay Kumar
    Article
    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94
    Insert
    Rac1 (RAC1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Constitutively Active, Q61L
    Promoter
    cumate-inducible promoter
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    84643-plasmid-map-sequence-id-175059

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  13. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  16. phCMV-229E-D15

    Plasmid
    #188908
    Purpose
    express truncated 229E spike for pseudovirus production
    Depositor
    David Nemazee
    Insert
    229E spike (S 229E)
    Expression
    Mammalian
    Mutation
    c-terminal 15aa deletion
    Promoter
    CMV
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188908-plasmid-map-sequence-id-370665

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  25. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Promoter
    pRhaBAD
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

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  30. pCMV-T7-EGFP (BPK1098)

    Plasmid
    #133962
    Purpose
    CMV and T7 promoter plasmid for EGFP expression in mammalian cells or for in vitro transcription
    Depositor
    Benjamin Kleinstiver
    Insert
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    133962-plasmid-map-sequence-id-265771

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Showing: 3001 - 3030 of 19771 results