159,352 results
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pCMV-T7 RNA POL
Plasmid#138352PurposeExpressing T7 RNA Polymerase in mammalian cellsDepositorInsertT7 RNA POL
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2prom::FLS2-3xmyc-GFP
Plasmid#86157PurposeFLS2-GFP expresssionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTags3xmyc and GFPExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max
Plasmid#112093PurposeC:G-to-T:A base editingDepositorInsertBE4max
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJJGL001
Plasmid#180605PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasXDepositorInsertDpbCasX
UseCRISPRTags10x His and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-YFP
Plasmid#13033PurposeMammalian expression vector for cloning your gene fused to YFPDepositorHas ServiceCloning Grade DNAInsertYellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
gRNA_AAVS1-T2
Plasmid#41818PurposeExpresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineeringDepositorInsertgRNA_AAVS1-T2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-TetOn-mCherry-OAZ1_FS-YFP
Plasmid#232356PurposeMammalian expression of dox-inducible polyamine sensor (polyamine levels = YFP/Cherry)DepositorInsertOAZ1 derived polyamine sensing module (OAZ1 Human)
UseLentiviralTagseYFP and mCherryExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-tetO-mCherry
Plasmid#70273PurposeLentiviral plasmid for tetracycline/doxycycline dependent expression of mCherryDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromotertetOAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_hCOL4A5-Nluc
Plasmid#198113PurposeExpress hCOL4A5 in mammalian cellsDepositorInsertCOL4A5 (COL4A5 Human)
UseTagsNlucExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLCK-mScarlet3_C1
Plasmid#189771PurposeConstruct for labelling plasma membranes in mammalian cells using LCK-mScarlet3DepositorInsertLCK-mScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8m-WPRE
Plasmid#162375PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationA25G F286YPromoterSynapsinAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-dPspCas13b-NES-mCherry
Plasmid#233609PurposeExpression of catalytically-inactive PspCas13b in mammalian cellsDepositorInsertdPspCas13b-NES-mCherry
UseCRISPRTags3xFlag and mCherryExpressionMutationH133A/H1058APromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-dPspCas13b-4EHP-NES-mCherry
Plasmid#233610PurposeExpression of catalytically-inactive PspCas13b fused with 4EHP in mammalian cellsDepositorInsertdPspCas13b-4EHP-NES-mCherry
UseCRISPRTags3xFlag and mCherryExpressionMutationH133A/H1058APromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_mNG2(11)_Clathrin light chain
Plasmid#82608PurposeExpresses mNG2(11) tagged clathrin light chain in mammalian cellsDepositorInsertmNG2(11)_Clathrin light chain
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-cys-His6-OtUBD
Plasmid#190091PurposeExpresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coliDepositorInsertOtUBD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)
Plasmid#185919PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
STR-KDEL_PSAP-SBP-mCherry
Plasmid#180440PurposeCo-expresses (via IRES) streptavidin-KDEL and Prosaposin-SBP-mCherryDepositorInsertProsaposin (PSAP )
UseTagsSBP-mCherryExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB-1
Plasmid#210387PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesTagsExpressionMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pME-QF
Plasmid#61375PurposeMiddle Gateway Entry Vector Containing QFDepositorInsertQF
UseTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
EZ-Tet-pLKO-Hygro
Plasmid#85972PurposeTet/Dox inducible shRNA lentivirus with hygromycin selectionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMutationPromoterH1 / TetOAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP/golgin-160
Plasmid#180630PurposeExpression of WT golgin-160DepositorInsertgolgin-160 (GOLGA3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-mAmetrine-CAAX
Plasmid#216132PurposeLentiviral vector for expressing mAmetrine localizing to the plasma membrane.DepositorInsertEF1a-mAmetrine-CAAX-WPRE
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-EglN1-pcDNA3
Plasmid#18963DepositorInsertEgg laying Nine - 1 (EGLN1 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
hBax C3-EGFP
Plasmid#19741DepositorInsertBax (BAX Human)
UseTagsGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3 APPA
Plasmid#207124PurposeLentiviral plasmid to express mouse Mettl3 APPA mutantDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationD395A and W398APromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_gRNA -hMLHdn-Puro
Plasmid#191105PurposeBackbone for lentiviral pegRNA library construction with hMLH1dn and Puro markerDepositorInserthMLH1dn
UseCRISPR and LentiviralTagsExpressionMammalianMutationI31 silent mutation (ATA to ATC) to remove early …PromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FRP795_insul-(lexA-box)8-PminCYC1-Citrine-TCYC1
Plasmid#58435PurposeInduces Citrine expression from a promoter containing 8 lexA boxes in yeast cellsDepositorInsertCitrineA206K
UseTagsExpressionYeastMutationPromoterinsul-(lexA-box)8-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only
