We narrowed to 41,926 results for: Tro;
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Plasmid#126547PurposeExpresses GFP-tagged RNF110 in mammalian cellsDepositorAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBI101-pGC1(D1)-GUS
Plasmid#49513PurposeExpresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plantDepositorInsertPromoter of GC1(D1) (AT1G22690 Mustard Weed)
UsePlant expressionTagsbeta-glucuronidasePromoterGC1 (At1g22690), 1141 bp upstream of the transcri…Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_OTmut
Plasmid#185385PurposeExpresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cellsDepositorInsertGPCR activation based oxytocin control sensor GRAB_OTmut
ExpressionMammalianPromoterCMVAvailable SinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4
Plasmid#123658PurposeDD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyExpressionMammalianPromotermU6Available SinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-U6ac-rac2-guides
Plasmid#168241Purpose"neutrophil specific GFP with ubiquitous rac2 sgRNAs"DepositorInsertrac2 sgRNAs
UseCRISPRAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-mSOG-bFos-IRES-mCherry
Plasmid#136599PurposeEncodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).DepositorInsertN-mSOG-bFos
ExpressionMammalianPromoterCMVAvailable SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-plk4
Plasmid#136788PurposeMammalian expression of the centrosomal protein plk4 N-terminally fused to SNAP-tagDepositorInsertSNAP-plk4 (PLK4 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EED_2
Plasmid#36386DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SETDB1_2
Plasmid#36348DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV FLIP p53
Plasmid#19745DepositorAvailable SinceDec. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pKM60
Plasmid#154159PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation4 wt NF-κB sites were replaced with Rel1A-specifi…Available SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMP-DNMT3A_1
Plasmid#36380DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t-gfp
Plasmid#48130PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-U6ac-cdk2-guides
Plasmid#168250Purpose"neutrophil specific GFP with ubiquitous cdk2 sgRNAs"DepositorInsertcdk2 sgRNAs
UseCRISPRAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1Xpress-Bcr-HisB
Plasmid#32508DepositorAvailable SinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD
Plasmid#25994DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pASK/3xMBT
Plasmid#28234DepositorInsertl(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)
TagsHisExpressionBacterialMutationTruncation expressing only aa 252-606 (MBT repeat…Available SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-RacFRET-U6ac-rac2 guides
Plasmid#168248Purpose"label active Rac under Rac2 neutrophil-specific knockout background"DepositorInsertRacFRET
UseZebrafish expressionPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 ERbeta(144-530)
Plasmid#35566DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEPQDKN0761
Plasmid#185630PurposeModified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 ERbeta(144-255)
Plasmid#35565DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MeCP2_1
Plasmid#36377DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 ERbeta(1-255)
Plasmid#35564DepositorAvailable SinceMarch 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP
Plasmid#183926PurposeOptogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposureDepositorAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfa28-nLac
Plasmid#53130PurposeExpression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains the A and B regions (bp -1757 to -1489) …Promoterhuman Gfa2Available SinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0107
Plasmid#177040PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0202
Plasmid#185626PurposeMoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoterDepositorInsertSpCas9
UseCRISPR and Synthetic BiologyAvailable SinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#154163PurposeFirefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseMutation20 wt NF-κB sites were replaced with Rel1A-specif…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM130Env
Plasmid#137665PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MeCP2_3
Plasmid#36379DepositorAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)
Plasmid#174639PurposeOptogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)DepositorInsertSSPB(micro)-GFP-ppKinVIb(861-1321)
TagsSSPB(micro)-GFPExpressionMammalianMutationSSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…PromoterChicken beta-actinAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-Ro2
Plasmid#24503DepositorInsertRASSL Ro2 (OPRK1 Human)
TagsFLAG and NLS from prolactinExpressionMammalianMutationmutations in extracellular loops 2 (see comments …Available SinceJune 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
Opto-casp9_casp9-VfAU1-LOV
Plasmid#131222PurposeExpresses Opto-casp9_casp9-VfAU1-LOV (light-activated caspase9)DepositorInsertOpto-casp9 (casp9-VfAU1-LOV)
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Opto-casp9_casp9-AtCRY2-PHR
Plasmid#131223PurposeOpto-casp9_casp9-AtCRY2-PHR (light-activated caspase9)DepositorInsertOpto-casp9 (casp9-AtCRY2-PHR)
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-plk4
Plasmid#136837PurposeMammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tagDepositorInsertCLIP-plk4 (PLK4 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM033Env
Plasmid#137672PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
ExpressionMammalianAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
UAS-u(XL)Cas9
Plasmid#127386PurposeExpresses Cas9 at very low levelDepositorInsertuORF(XL)-Cas9
UseCRISPRExpressionInsectPromoterUASTAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1-gfp
Plasmid#48133PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nanog-t2A-mCherry
Plasmid#127541PurposeExpresses the transactivation domain of mouse Nanog and mCherry via t2A linker.DepositorInsertNanog (Nanog Mouse)
ExpressionMammalianMutationCodes for the last 110 a.a of NanogPromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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mADAM8 deltaMP in pQCXIP cFLAG
Plasmid#19149DepositorAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T2
Plasmid#97328PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T2
Tags6xHis - TEVExpressionBacterialMutationAddition of two T residues after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-CCCAP
Plasmid#136847PurposeMammalian expression of the centrosomal protein CCCAP N-terminally fused to CLIP-tagDepositorInsertCLIP-CCCAP (SDCCAG8 Human, Synthetic)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1Xpress-BcrR1090A-HisB
Plasmid#32507DepositorAvailable SinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t-gfp
Plasmid#48131PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 T538N
Plasmid#60537PurposeDonor vector for human Pak5 T538N mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 PAK5 S573N
Plasmid#60538PurposeDonor vector for human Pak5 S573N mutantDepositorAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only