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pORTMAGE-Pa1

Plasmid

#138475

Purpose

Enables high-efficiency recombineering in P. aeruginosa

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

PapRecT
mutL E36K

Expression

Bacterial

Mutation

E36K

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 NQO1

Plasmid

#61730

Purpose

Expresses WT human NQO1

Depositor

Yosef Shaul

Article

Adamovich et al Mol Cell Biol. 2013 Jul;33(13):2603-13.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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p10xQUAS-CsChrimson

Plasmid

#163629

Purpose

10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

Depositor

Christopher Potter

Article

Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

Insert

CsChrimson.mVenus

Tags

mVenus

Expression

Insect

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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WNK2 K207A

Plasmid

#24570

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2` (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Mutation

K207A

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pET28-At_PRORP2-His

Plasmid

#67870

Purpose

bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP2 (PRORP2 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCsy-crRNA-EV

Plasmid

#153944

Purpose

For cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cells

Depositor

Zhou Songyang

Article

Chen et al Nat Commun. 2020 Jun 19;11(1):3136. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-At_PRORP3-His

Plasmid

#67871

Purpose

bacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tag

Depositor

Walter Rossmanith

Article

Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

Insert

PRORP3 (PRORP3 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAWp9-5

Plasmid

#73856

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAWp9-4

Plasmid

#73855

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pET-15b-aδNb072

Plasmid

#204627

Purpose

Expresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.

Depositor

James McDonnell

Article

Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

Insert

aδNb072

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARP-myc

Plasmid

#185517

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Mutation

Replaces Q to P in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-GARP

Plasmid

#185516

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

Insert

foxd4l1.1

Use

In vitro transcription

Mutation

Replaces Q to P in GARQYNLI

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::LNP1-ires-GFP

Plasmid

#237494

Purpose

Express NUP98::LNP1 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::LNP1 (LNP1, NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::HOXA9-ires-GFP

Plasmid

#237484

Purpose

Express NUP98::HOXA9 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::HOXA9 (HOXA9, NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98::SETBP1

Plasmid

#237491

Purpose

Express HA-tagged NUP98::SETBP1 in dual promoter lentiviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98::SETBP1 (NUP98, SETBP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

MND/PGK

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::NSD1-ires-GFP

Plasmid

#237483

Purpose

Express NUP98::NSD1 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::NSD1 (NUP98, NSD1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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SHP2(WT)@pLKO-Trex-SBP

Plasmid

#85457

Purpose

Gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Promoter

RSV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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SHP2(C459S)@pLKO-Trex-HA

Plasmid

#85459

Purpose

Expresses SHP2 mutant in mammalian cells

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

C459S

Promoter

RSV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
SHP2(T253M/Q257L)@pLKO-Trex-SBP

Plasmid

#85458

Purpose

SHP2 mutant gene expression

Depositor

Darrin Stuart

Article

Chen et al Nature. 2016 Jul 7;535(7610):148-52.

Insert

SHP2 (PTPN11 Human)

Use

Lentiviral

Tags

SBP Tag

Expression

Mammalian

Mutation

T253M/Q257L

Promoter

RSV

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::RAP1GDS1-ires-GFP

Plasmid

#237485

Purpose

Express NUP98::RAP1GDS1 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::RAP1GDS1 (NUP98, RAP1GDS1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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